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ItemCurrent state-of-the-art methods, equipment and analyses in soil ecotoxicology( 2022)Soil ecotoxicology study the impact of pollutants on soil ecosystem, which has been developed for more than 50 years. After the first soil ecotoxicology study published in 1960s, the methodology of soil ecotoxicity test was rapidly developed to support the ecological risk assessment. Many standardized ecotoxicity tests were established by international organizations (OECD and ISO) to provide researchers and industries to produce a comparable ecotoxicity data that can improve the understanding of mechanisms of toxicity. However, researchers started to notice the limitations of standardized ecotoxicity test to solve more complicated pollution issues raised in recent years. These issues include the reproducibility and reliability of standard ecotoxicity test, the limited information gained from ecotoxicity test, and the challenge in ecological risk assessment from laboratory experiments to field study. Currently, several novel methodologies, technologies, and analysis in soil ecotoxicology, such as full life cycle ecotoxicity test, continuous monitoring techniques, and eDNA metabarcoding, are proposed as a potential tools used in ecological risk assessment. These novel developments have high potential to deal with the challenges raised in soil ecotoxicology, while no literature review provide a holistic view to summarize them and link with modern pollution issues. Therefore, this article introduce the the historical perspective of soil ecotoxicology, summarize the pollution issues raised in recent years, and point out the state-of-the-art methodology, technology, and analysis in soil ecotoxicology.