- Chemical and Biomolecular Engineering - Research Publications
Chemical and Biomolecular Engineering - Research Publications
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ItemInheritance of chromosome 7 is associated with a drug-resistant phenotype in somatic cell hybridsdeSilva, M ; Kantharidis, P ; Wall, DM ; Campbell, L ; Vrazas, V ; Nadalin, G ; Kaczmarczyk, SJ ; Hu, XF ; Parkin, JD ; Zalcberg, JR (STOCKTON PRESS, 1996-01)A major form of drug resistance in tumour cells known as classical multidrug resistance (MDR) is associated with the overexpression of the mdr1 gene product, the membrane protein P-glycoprotein (P-gp), which acts as an energy-dependent drug efflux pump. In this study the inheritance of P-gp expression was examined using hybrids formed after somatic cell fusion between a drug-sensitive human T-cell leukaemia cell line, CEM/CCRF, and a drug-resistant derivative, CEM/A7, which is characterized by a clonal chromosomal duplication dup(7)(q11.23q31.2). Fourteen hybrids, chosen at random, were analysed by reverse transcriptase-polymerase chain reaction (RT-PCR) and by binding studies involving the monoclonal antibody MRK16, which recognises an external P-gp epitope. Only two hybrids were positive for both MRK16 antibody labelling and mdr1 mRNA. Partial karyotypic analysis of all hybrids revealed that only the MRK16-positive hybrids contained the duplication in chromosome 7 seen in the CEM/A7 parental MDR line. Therefore, P-gp overexpression in the MRK16-positive hybrids may be linked to the inheritance of chromosome 7 from CEM/A7 and possibly associated with the chromosome 7 abnormality.
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ItemNo Preview AvailableINVESTIGATION OF IMMUNE-REACTIONS IN A FLOW-INJECTION SYSTEM USING SURFACE-PLASMON RESONANCECARUSO, F ; VUKUSIC, PS ; MATSUURA, K ; URQUHART, RS ; FURLONG, DN ; OKAHATA, Y (ELSEVIER SCIENCE BV, 1995-10-10)
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ItemNo Preview AvailableA quartz crystal microbalance study of the removal of solid organic soils from a hard surface in aqueous surfactant solutionWeerawardena, A ; Drummond, CJ ; Caruso, F ; McCormick, M (ELSEVIER SCIENCE BV, 1999-01-15)
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ItemNo Preview AvailableDNA binding and hybridization on gold and derivatized surfacesCaruso, F ; Rodda, E ; Furlong, DN ; Haring, V (ELSEVIER SCIENCE SA, 1997-06-30)
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ItemNo Preview AvailableOrientational aspects of antibody immobilization and immunological activity on quartz crystal microbalance electrodesCaruso, F ; Rodda, E ; Furlong, DN (ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS, 1996-03-01)
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ItemNo Preview AvailableSURFACE CHEMICAL ACTIVATION OF QUARTZ-CRYSTAL MICROBALANCE GOLD ELECTRODES - ANALYSIS BY FREQUENCY CHANGES, CONTACT-ANGLE MEASUREMENTS AND GRAZING ANGLE FTIRGEDDES, NJ ; PASCHINGER, EM ; FURLONG, DN ; CARUSO, F ; HOFFMANN, CL ; RABOLT, JF (ELSEVIER SCIENCE SA LAUSANNE, 1995-05-15)
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ItemNo Preview AvailableIMMOBILIZATION OF IGG ONTO GOLD SURFACES AND ITS INTERACTION WITH ANTI-IGG STUDIED BY SURFACE-PLASMON RESONANCEGEDDES, NJ ; MARTIN, AS ; CARUSO, F ; URQUHART, RS ; FURLONG, DN ; SAMBLES, JR ; THAN, KA ; EDGAR, JA (ELSEVIER SCIENCE BV, 1994-10-14)The optical excitation of surface plasmon resonance (SPR) at a metal dielectric interface has been used to study the binding of immunoglobulin G (IgG) to gold and anti-IgG to immobilised IgG layers. In these studies both a monoclonal mouse and polyclonal sheep IgG were used as receptor layers for anti-IgG. The kinetics of binding were investigated by monitoring the reflectivity of light at an angle close to plasmon resonance. Both the initial rate of change and final reflectivity were measured during and after protein binding. The amount of protein bound to the surface was found to be less for the monoclonal mouse IgG compared to the polyclonal sheep IgG, these two IgG nominally being of the same dimensions and molecular weight. Further, anti-IgG binding produced greater changes in reflectivity than the initial IgG layers. By fitting the full angle-dependent reflectivity data to the Fresnel equation the effective protein layer thicknesses of IgG and anti-IgG as a function of concentration were determined. Differences in the effective thickness of the bound layer for the two IgG was observed, the mouse IgG having a thinner effective thickness compared with the sheep IgG. The limitations of direct binding of protein to metal surfaces in SPR biosensor applications are discussed.
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ItemNo Preview AvailableIn-situ measurement of DNA immobilization and hybridization using a 27 MHz quartz crystal microbalanceCaruso, F ; Furlong, DN ; Niikura, K ; Okahata, Y (ELSEVIER SCIENCE BV, 1998-03-15)
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ItemA NEW, REGIOSELECTIVE, TANDEM AMIDATION REACTION OF ELECTRON-RICH ARENESCABLEWSKI, T ; GURR, PA ; RANER, KD ; STRAUSS, CR (AMER CHEMICAL SOC, 1994-09-23)