Biochemistry and Pharmacology - Research Publications

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Author: Gleeson, Paul × Author: Hatters, Daniel × End date: 2012 × Start date: 2012 × Type: Journal Article ×

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    Cargo sorting and endosome-to-Golgi retrograde transport pathways.
    Chia, PZ ; Houghton, FJ ; Hatters, DM ; Gleeson, PA (AMER SOC CELL BIOLOGY, 2012)
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    Tracking protein aggregation and mislocalization in cells with flow cytometry
    Ramdzan, YM ; Polling, S ; Chia, CPZ ; Ng, IHW ; Ormsby, AR ; Croft, NP ; Purcell, AW ; Bogoyevitch, MA ; Ng, DCH ; Gleeson, PA ; Hatters, DM (NATURE PUBLISHING GROUP, 2012-05)
    We applied pulse-shape analysis (PulSA) to monitor protein localization changes in mammalian cells by flow cytometry. PulSA enabled high-throughput tracking of protein aggregation, translocation from the cytoplasm to the nucleus and trafficking from the plasma membrane to the Golgi as well as stress-granule formation. Combining PulSA with tetracysteine-based oligomer sensors in a cell model of Huntington's disease enabled further separation of cells enriched with monomers, oligomers and inclusion bodies.