School of BioSciences - Research Publications

Permanent URI for this collection

http://hdl.handle.net/11343/57446

Filters

2017 1
Reset filters

Settings
Statistics
Citations
Start date: 2010 × End date: 2019 × Type: Chapter × Author: Bacic, Anthony ×

Search Results

Now showing 1 - 1 of 1
  • Item
    Thumbnail Image
    Enrichment of Golgi Membranes from Triticum aestivum (Wheat) Seedlings
    Zeng, W ; Ebert, B ; Parsons, HT ; Rautengarten, C ; Bacic, A ; Heazlewood, JL ; Taylor, NL ; Millar, AH (Humana Press, 2017)
    The Golgi apparatus is an essential component in the plant secretory pathway. The enrichment of Golgi membranes from plant tissue is fundamental to the study of this structurally complex organelle. The utilization of density centrifugation for the enrichment of Golgi membranes is still the most widely employed isolation technique. Generally, the procedure requires optimization depending on the plant tissue being employed. Here we provide a detailed enrichment procedure that has previously been used to characterize cell wall biosynthetic complexes from wheat seedlings. We also outline several downstream analyses procedures, including nucleoside diphosphatase assays, immunoblotting, and finally localization of putative Golgi proteins by fluorescent tags.