Computing and Information Systems - Theses

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Subject: machine learning × Subject: computational biology × Subject: bioinformatics × Author: Ho, Hui Kian × Type: PhD thesis ×

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    Computational substructure querying and topology prediction of the beta-sheet
    Ho, Hui Kian ( 2014)
    Studying the three-dimensional structure of proteins is essential to understanding their function, and ultimately, their dysfunction that causes disease. The limitations of experimental protein structure determination presents a need for computational approaches to protein structure prediction and analysis. The beta-sheet is a commonly occurring protein substructure important to many biological processes and are often implicated in neurological disorders. Targeted experimental studies of beta-sheets are especially difficult due to their general insolubility in isolation. This thesis presents a series of contributions to the computational analysis and prediction of beta-sheet structure, which are useful for knowledge discovery and for directing more detailed experimental work. Approaches for predicting the simplest type of beta-sheet, the beta-hairpin, are first described. Improvements over existing methods are obtained by using the most important beta-hairpin features identified through systematic feature selection. An examination of the most important features provides a physiochemical basis of their usefulness in beta-hairpin prediction. New methods for the more general problem of beta-sheet topology prediction are described. Unlike recent methods, ours are independent of multiple sequence alignment (MSAs) and therefore do not rely on the coverage of reference sequence databases or sequence homology. Our evaluations showed that our methods do not exhibit the same reductions in performance as a state-of-the-art method for sequences with low quality MSAs. A new method for the indexing and querying of beta-sheet substructures, called BetaSearch, is described. BetaSearch exploits the inherent planar constraints of beta-sheet structure to achieve significant speedups over existing graph indexing and conventional 3D structure search methods. Case studies are presented that demonstrate the potential of this method for the discovery of biologically interesting beta-sheet substructures. Finally, a purpose-built open source toolkit for generating 2D protein maps is described, which is useful for the coarse-grained analysis and visualisation of 3D protein structures. It can also be used in existing knowledge discovery pipelines for automated structural analysis and prediction tasks, as a standalone application, or imported into existing experimental applications.