Academic Services and Registrar - Research Publications
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ItemStyling Against Absolute Knowledge in Derrida's 'Glas'(Open Humanities Press, 2015)
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ItemExpression of muscarinic receptor subtypes in tree shrew ocular tissues and their regulation during the development of myopia(MOLECULAR VISION, 2009-03-02)PURPOSE: Muscarinic receptors are known to regulate several important physiologic processes in the eye. Antagonists to these receptors such as atropine and pirenzepine are effective at stopping the excessive ocular growth that results in myopia. However, their site of action is unknown. This study details ocular muscarinic subtype expression within a well documented model of eye growth and investigates their expression during early stages of myopia induction. METHODS: Total RNA was isolated from tree shrew corneal, iris/ciliary body, retinal, choroidal, and scleral tissue samples and was reverse transcribed. Using tree shrew-specific primers to the five muscarinic acetylcholine receptor subtypes (CHRM1-CHRM5), products were amplified using polymerase chain reaction (PCR) and their identity confirmed using automated sequencing. The expression of the receptor proteins (M1-M5) were also explored in the retina, choroid, and sclera using immunohistochemistry. Myopia was induced in the tree shrew for one or five days using monocular deprivation of pattern vision, and the expression of the receptor subtypes was assessed in the retina, choroid, and sclera using real-time PCR. RESULTS: All five muscarinic receptor subtypes were expressed in the iris/ciliary body, retina, choroid, and sclera while gene products corresponding to CHRM1, CHRM3, CHRM4, and CHRM5 were present in the corneal samples. The gene expression data were confirmed by immunohistochemistry with the M1-M5 proteins detected in the retina, choroid, and sclera. After one or five days of myopia development, muscarinic receptor gene expression remained unaltered in the retinal, choroidal, and scleral tissue samples. CONCLUSIONS: This study provides a comprehensive profile of muscarinic receptor gene and protein expression in tree shrew ocular tissues with all receptor subtypes found in tissues implicated in the control of eye growth. Despite the efficacy of muscarinic antagonists at inhibiting myopia development, the genes of the muscarinic receptor subtypes are neither regulated early in myopia (before measurable axial elongation) nor after significant structural change.
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ItemDesigning online orientations for higher education music students: A proposed framework(ASCILITE, 2018)Online orientations can provide university students with helpful introductions to relevant knowledge and skills they will need over the course of their studies. While traditional models of university orientation focus on face-to-face lecture delivery and often depend on individual, time-specified events, the online environment can be used for more interactive and discipline-specific orientation. The adoption of an online orientation approach can further provide students with information accessible in manageable time frames and supportive practical applications. Aligned to research literature, this paper proposes a framework for developing an online orientation program for higher education undergraduate and graduate music students. The framework brings together the design benefits of the online environment in conjunction with literature on effective practices of orientation programs. As such, the framework identifies four components of influence when designing an online orientation: Purpose; Audience; Design construction; and Content topic considerations. Areas for future research are also highlighted.
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ItemVolunteers in Australian archives(ROUTLEDGE JOURNALS, TAYLOR & FRANCIS LTD, 2017)Why do volunteers choose to contribute thousands of unpaid hours per week to Australian archives? This paper presents the results of a nationwide web survey that provides insight into the demographics, motivations and experiences of volunteers in Australian archives. The findings provide a representative overview of formal volunteers in Australian archives, determining ‘who’ they are, the value of the contributions they provide and the level of training and support offered to them. This study is a continuation of existing discussions about volunteers in Australian archives and represents an opportunity for the development of stronger relationships with Australian archival volunteers and, through them, the communities our archives serve.
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ItemNo Preview AvailableReimagining the performance experience through the establishment of a virtual performance hall.( 2021-10-27)Paper presented as part of the symposium "Reimagining Performance with Immersive Reality Technologies" at ISPS 2021, 27-30 October, Montreal, Canada.
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ItemDesign principles for immersive reality to enhance creativity and performance through transdisciplinary collaboration.( 2021-10-27)Paper presented as part of the symposium "Reimagining Performance with Immersive Reality Technologies" at ISPS 2021, 27-30 October, Montreal, Canada.
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ItemReimagining music performance with immersive reality technologies.( 2021-11-05)NowNet Arts Conference 2021 Network Arts: Transformation of Distance.
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ItemNo Preview AvailableExploring opportunities for musician’s health and performance enhancement using VR simulation training.( 2021-10-07)Paper presented at Games for Change Asia-Pacific Festival 2021, 5-7 October, online conference.
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ItemNo Preview AvailableApplication profile for machine-actionable data management plans(Ubiquity Press, Ltd., 2021-01-01)
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ItemNo Preview AvailableThe major membrane nuclease MnuA degrades neutrophil extracellular traps induced by Mycoplasma bovis(ELSEVIER SCIENCE BV, 2018-05)Mycoplasma bovis has been increasingly recognised worldwide as an economically important pathogen of cattle, causing a range of diseases, including pneumonia, mastitis, polyarthritis and otitis media. It is believed that M. bovis utilises a range of cell surface proteins, including nucleases, to evade the host immune response and survive. However, despite the importance of neutrophils in controlling pathogenic bacteria, the interaction between these cells and M. bovis is not well-characterised. In addition to phagocytosis, neutrophils combat pathogens through the release of neutrophil extracellular traps (NETs), which are composed of their nuclear and granular components, including DNA. Here we investigated the effect of the major membrane nuclease MnuA of M. bovis, which in vitro is responsible for the majority of the nuclease activity of M. bovis, on NET formation. We quantified NET formation by bovine neutrophils 4 h after stimulation with wild-type M. bovis, an mnuA mutant and a mnuA-pIRR45 complemented mnuA mutant. NETs were detected following stimulation of neutrophils with the mnuA mutant but not after exposure to either the wild-type or the mnuA-pIRR45 complemented mutant, and NETs were degraded in the presence of even low concentrations of wild type M. bovis. Surprisingly, there was no increase in levels of intracellular reactive oxygen species (ROS) production in neutrophils stimulated with M. bovis, even though these neutrophils produced NETs. These results clearly demonstrate that M. bovis can induce NET formation in bovine neutrophils, but that the major membrane nuclease MnuA is able to rapidly degrade NETs, and thus is likely to play a significant role in virulence. In addition, M. bovis appears to induce NETs even though ROS production seems to be suppressed.