Academic Services and Registrar - Research Publications

Permanent URI for this collection

http://hdl.handle.net/11343/192991

Filters

2009 5
Reset filters

Settings
Statistics
Citations
Type: Journal Article × Start date: 2009 × End date: 2009 ×

Search Results

Now showing 1 - 5 of 5
  • Item
    Thumbnail Image
    Expression of muscarinic receptor subtypes in tree shrew ocular tissues and their regulation during the development of myopia
    McBrien, NA ; Jobling, AI ; Truong, HT ; Cottriall, CL ; Gentle, A (MOLECULAR VISION, 2009-03-02)
    PURPOSE: Muscarinic receptors are known to regulate several important physiologic processes in the eye. Antagonists to these receptors such as atropine and pirenzepine are effective at stopping the excessive ocular growth that results in myopia. However, their site of action is unknown. This study details ocular muscarinic subtype expression within a well documented model of eye growth and investigates their expression during early stages of myopia induction. METHODS: Total RNA was isolated from tree shrew corneal, iris/ciliary body, retinal, choroidal, and scleral tissue samples and was reverse transcribed. Using tree shrew-specific primers to the five muscarinic acetylcholine receptor subtypes (CHRM1-CHRM5), products were amplified using polymerase chain reaction (PCR) and their identity confirmed using automated sequencing. The expression of the receptor proteins (M1-M5) were also explored in the retina, choroid, and sclera using immunohistochemistry. Myopia was induced in the tree shrew for one or five days using monocular deprivation of pattern vision, and the expression of the receptor subtypes was assessed in the retina, choroid, and sclera using real-time PCR. RESULTS: All five muscarinic receptor subtypes were expressed in the iris/ciliary body, retina, choroid, and sclera while gene products corresponding to CHRM1, CHRM3, CHRM4, and CHRM5 were present in the corneal samples. The gene expression data were confirmed by immunohistochemistry with the M1-M5 proteins detected in the retina, choroid, and sclera. After one or five days of myopia development, muscarinic receptor gene expression remained unaltered in the retinal, choroidal, and scleral tissue samples. CONCLUSIONS: This study provides a comprehensive profile of muscarinic receptor gene and protein expression in tree shrew ocular tissues with all receptor subtypes found in tissues implicated in the control of eye growth. Despite the efficacy of muscarinic antagonists at inhibiting myopia development, the genes of the muscarinic receptor subtypes are neither regulated early in myopia (before measurable axial elongation) nor after significant structural change.
  • Item
    Thumbnail Image
    Digitising and archiving Syliphone recordings in Guinea
    Counsel, G (African Studies Association of Australasia and the Pacific (AFSAAP), 2009)
  • Item
  • Item
    Thumbnail Image
    Archival and Research Resources in Conakry, Guinea
    Counsel, G (Cambridge University Press (CUP), 2009)
    Archival research in West Africa can present many challenges. In one of the poorest regions of the world, governments struggle to maintain funding for the most basic infrastructure; thus archives and their holdings can often reside in a neglected state. Moreover, research materials may be spread over many departments and buildings, creating a labyrinthine network of officialdom, and requisite access requirements. This paper provides a brief overview of the principal archives located in Conakry, capital of Guinea. It includes current contact information and descriptions of holdings, and expands upon earlier articles by Klein (1981), Ford (1987), Conrad (1993), and Sampson (2002). On 2 October 2008 Guinea celebrated 50 years of independence. Earlier that year many new ministries were created, including the Ministère de la Culture, des Arts et Loisirs. For the first time in the nation's history, Guinea had its own dedicated ministry of the arts, thus potentially streamlining academic and archival research. The new Ministry faced many hurdles, however, for the story of Guinea's archives during the last 50 years is a tale of both marvel and neglect. During the nation's First Republic (1958-1984), Guinea's archives became established under the Presidency of Sékou Touré. The Archives Nationale, the Bibliothèque Nationale, and Radiodiffusion Télévision de Guinée were all created during his reign, and Guinea's archival resources were said to be among the best in West Africa. As Touré's grip on power strengthened, the nation's economic malaise grew, and Klein (1981:333) reported that in the early 1980s, when he conducted research in Conakry, he had been warned to expect significant damage to the archival contents.
  • Item