Electrical and Electronic Engineering - Research Publications

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Author: ABEYRATHNE, CHATHURIKA × Author: Kwan, Patrick × Author: Skafidas, Efstratios × Author: Uddin, Shah Mukim × End date: 2022 ×

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    Ultrasensitive and label-free biosensor for the detection of Plasmodium falciparum histidine-rich protein II in saliva.
    Soraya, GV ; Abeyrathne, CD ; Buffet, C ; Huynh, DH ; Uddin, SM ; Chan, J ; Skafidas, E ; Kwan, P ; Rogerson, SJ (Nature Publishing Group, 2019-11-25)
    Malaria elimination is a global public health priority. To fulfil the demands of elimination diagnostics, we have developed an interdigitated electrode sensor platform targeting the Plasmodium falciparum Histidine Rich Protein 2 (PfHRP2) protein in saliva samples. A protocol for frequency-specific PfHRP2 detection in phosphate buffered saline was developed, yielding a sensitivity of 2.5 pg/mL based on change in impedance magnitude of the sensor. This protocol was adapted and optimized for use in saliva with a sensitivity of 25 pg/mL based on change in resistance. Further validation demonstrated detection in saliva spiked with PfHRP2 from clinical isolates in 8 of 11 samples. With a turnaround time of ~2 hours, the label-free platform based on impedance sensors has the potential for miniaturization into a point-of-care diagnostic device for malaria elimination.