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Start date: 2021 × End date: 2021 × Type: Journal Article × Author: McCluskey, James × Author: Eckle, Sidonia ×

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    Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)
    Cossarizza, A ; Chang, H-D ; Radbruch, A ; Abrignani, S ; Addo, R ; Akdis, M ; Andrae, I ; Andreata, F ; Annunziato, F ; Arranz, E ; Bacher, P ; Bari, S ; Barnaba, V ; Barros-Martins, J ; Baumjohann, D ; Beccaria, CG ; Bernardo, D ; Boardman, DA ; Borger, J ; Boettcher, C ; Brockmann, L ; Burns, M ; Busch, DH ; Cameron, G ; Cammarata, I ; Cassotta, A ; Chang, Y ; Chirdo, FG ; Christakou, E ; Cicin-Sain, L ; Cook, L ; Corbett, AJ ; Cornelis, R ; Cosmi, L ; Davey, MS ; De Biasi, S ; De Simone, G ; del Zotto, G ; Delacher, M ; Di Rosa, F ; Di Santo, J ; Diefenbach, A ; Dong, J ; Doerner, T ; Dress, RJ ; Dutertre, C-A ; Eckle, SBG ; Eede, P ; Evrard, M ; Falk, CS ; Feuerer, M ; Fillatreau, S ; Fiz-Lopez, A ; Follo, M ; Foulds, GA ; Froebel, J ; Gagliani, N ; Galletti, G ; Gangaev, A ; Garbi, N ; Garrote, JA ; Geginat, J ; Gherardin, NA ; Gibellini, L ; Ginhoux, F ; Godfrey, DI ; Gruarin, P ; Haftmann, C ; Hansmann, L ; Harpur, CM ; Hayday, AC ; Heine, G ; Hernandez, DC ; Herrmann, M ; Hoelsken, O ; Huang, Q ; Huber, S ; Huber, JE ; Huehn, J ; Hundemer, M ; Hwang, WYK ; Iannacone, M ; Ivison, SM ; Jaeck, H-M ; Jani, PK ; Keller, B ; Kessler, N ; Ketelaars, S ; Knop, L ; Knopf, J ; Koay, H-F ; Kobow, K ; Kriegsmann, K ; Kristyanto, H ; Krueger, A ; Kuehne, JF ; Kunze-Schumacher, H ; Kvistborg, P ; Kwok, I ; Latorre, D ; Lenz, D ; Levings, MK ; Lino, AC ; Liotta, F ; Long, HM ; Lugli, E ; MacDonald, KN ; Maggi, L ; Maini, MK ; Mair, F ; Manta, C ; Manz, RA ; Mashreghi, M-F ; Mazzoni, A ; McCluskey, J ; Mei, HE ; Melchers, F ; Melzer, S ; Mielenz, D ; Monin, L ; Moretta, L ; Multhoff, G ; Munoz, LE ; Munoz-Ruiz, M ; Muscate, F ; Natalini, A ; Neumann, K ; Ng, LG ; Niedobitek, A ; Niemz, J ; Almeida, LN ; Notarbartolo, S ; Ostendorf, L ; Pallett, LJ ; Patel, AA ; Percin, GI ; Peruzzi, G ; Pinti, M ; Pockley, AG ; Pracht, K ; Prinz, I ; Pujol-Autonell, I ; Pulvirenti, N ; Quatrini, L ; Quinn, KM ; Radbruch, H ; Rhys, H ; Rodrigo, MB ; Romagnani, C ; Saggau, C ; Sakaguchi, S ; Sallusto, F ; Sanderink, L ; Sandrock, I ; Schauer, C ; Scheffold, A ; Scherer, HU ; Schiemann, M ; Schildberg, FA ; Schober, K ; Schoen, J ; Schuh, W ; Schueler, T ; Schulz, AR ; Schulz, S ; Schulze, J ; Simonetti, S ; Singh, J ; Sitnik, KM ; Stark, R ; Starossom, S ; Stehle, C ; Szelinski, F ; Tan, L ; Tarnok, A ; Tornack, J ; Tree, TIM ; van Beek, JJP ; van de Veen, W ; van Gisbergen, K ; Vasco, C ; Verheyden, NA ; von Borstel, A ; Ward-Hartstonge, KA ; Warnatz, K ; Waskow, C ; Wiedemann, A ; Wilharm, A ; Wing, J ; Wirz, O ; Wittner, J ; Yang, JHM ; Yang, J (WILEY, 2021-12)
    The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples with examples of flow cytometry applications in the context of autoimmune diseases, cancers as well as acute and chronic infectious diseases. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer-reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state-of-the-art handbook for basic and clinical researchers.
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    Francisella tularensis induces Th1 like MAIT cells conferring protection against systemic and local infection
    Zhao, Z ; Wang, H ; Shi, M ; Zhu, T ; Pediongco, T ; Lim, XY ; Meehan, BS ; Nelson, AG ; Fairlie, DP ; Mak, JYW ; Eckle, SBG ; Moreira, MDL ; Tumpach, C ; Bramhall, M ; Williams, CG ; Lee, HJ ; Haque, A ; Evrard, M ; Rossjohn, J ; McCluskey, J ; Corbett, AJ ; Chen, Z (NATURE PORTFOLIO, 2021-07-16)
    Mucosal-associated Invariant T (MAIT) cells are recognized for their antibacterial functions. The protective capacity of MAIT cells has been demonstrated in murine models of local infection, including in the lungs. Here we show that during systemic infection of mice with Francisella tularensis live vaccine strain results in evident MAIT cell expansion in the liver, lungs, kidney and spleen and peripheral blood. The responding MAIT cells manifest a polarised Th1-like MAIT-1 phenotype, including transcription factor and cytokine profile, and confer a critical role in controlling bacterial load. Post resolution of the primary infection, the expanded MAIT cells form stable memory-like MAIT-1 cell populations, suggesting a basis for vaccination. Indeed, a systemic vaccination with synthetic antigen 5-(2-oxopropylideneamino)-6-D-ribitylaminouracil in combination with CpG adjuvant similarly boosts MAIT cells, and results in enhanced protection against both systemic and local infections with different bacteria. Our study highlights the potential utility of targeting MAIT cells to combat a range of bacterial pathogens.