Graeme Clark Collection
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ItemLIF potentiates the NT-3-mediated survival of spiral ganglia neurones in vitro( 1997)The survival of auditory neurones depends on the continued supply of trophic factors. Early postnatal spiral ganglion cells (SGC) in a dissociated cell culture were used as a model of auditory innervation to test the trophic factors leukaemia inhibitory factor (LIF) and neurotrophin-3 (NT-3) for their ability, individually or in combination, to promote neuronal survival. The findings suggest that LIF supports neuronal survival in a concentration-dependent manner. Moreover LIF potentiated NT-3-mediated spiral ganglion neuronal survival in a synergistic fashion.
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ItemThe neurotrophins act synergistically with LIF and members of the TGF-β superfamily to promote the survival of spiral ganglia neurons in vitro( 1999)Unavailable due to copyright.
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ItemSynergy between TGF-ß3 and NT-3 to promote the survival of spiral ganglia neurones in vitro( 1998)Transforming growth factor-βs (TGF-βs) have been implicated in normal inner ear development and in promoting neuronal survival. Early rat post-natal spiral ganglion cells (SGC) in dissociated cell culture were used as a model of auditory innervation to test the trophic factors TGF-βs and neurotrophin-3 (NT-3) for their ability, individually or in combination, to promote neuronal survival. The findings from this study suggest that TGF-βs supports neuronal survival in a concentration-dependent manner. Moreover TGF-βs and NT-3-potentiated spiral ganglion neuronal survival in a synergistic fashion.
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ItemThe interactions between the cytokine LIF and the neurotrophins on spiral ganglion cells(Monduzzi Editore, 1997)The survival of auditory neurones depends on the continued supply of trophic factors. Early post-natal spiral ganglion cells (SGC) in a dissociated cell culture were used as a model of auditory innervation to test the trophic factors leukaemia inhibitory factor (LIF) and neurotrophin-3 (NT-3) for their ability, individually or in combination, to promote neuronal survival.
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ItemThe interaction between the cytokine LIF and the neurotrophins on spiral ganglion cells [Abstract]( 1997)The survival of auditory neurones depends on the continuous supply of trophic factors. Hair cells within the cochlea are known to produce supply growth factors responsible for the survival and growth of The survival of auditory neurones depends on the continuous supply of trophic factors. Hair cells within the cochlea are known to produce supply growth factors responsible for the survival and growth of [ ] neurones. As a result of trauma, disease or ageing cochlear hair cells are lost, and consequently, the supply of growth factors is reduced, leading to secondary wave of auditory nerve degeneration.
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ItemIncreased survival of auditory neurones treated with LIF( 1997)Degeneration of spiral ganglion cells (SOC) is one of the most common correlates of sensorineural hearing loss (1). Several lines of evidence show that the continued supply of growth factors is responsible for maintaining auditory neurone integrity (2). In the present study SOC cultures were used as a model of auditory innervation to test the ability of the cytokine leukaemia inhibitory factor (LIF) and the neurotrophin NT -3 to promote neuronal survival individually or in combination. The data demonstrate that LIF promotes the survival of SOC in a concentration-dependent manner, with a significant increase in neuronal survival at concentrations as low as 0.1 ng/ml compared to untreated wells ( p< 0.05), and a maximum neuronal survival at 10 ng/ml. In addition, when used in combination LIF and NT-3 were more effective in promoting neuronal survival than either factor individually, with a significant increase in survival at concentrations of 0.1ng mI[to the power of]-1/0.1 ng mI[to the power of]-1 (LIF/NT-3). To our knowledge this is the first study reporting that LIF has trophic activity on SOC. Moreover, the data suggest that a combination of several growth factors may provide a better approach when developing pharmacological therapies for auditory neuron repair.