Veterinary Biosciences - Research Publications

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Author: Kimpton, Wayne × Author: Washington, Elizabeth × End date: 2009 × Start date: 2003 ×

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    Tracking dendritic cells:: use of an in situ method to label all blood leukocytes
    Ristevski, B ; Young, AJ ; Dudler, L ; Cahill, RNP ; Kimpton, W ; Washington, E ; Hay, JB (OXFORD UNIV PRESS, 2003-02)
    Here we describe an in situ procedure with a labeling index (percent of labeled blood leukocytes) >98%, which is high enough to permit the direct tracking of dendritic cell (DC) precursors from blood into lymphoid tissues, while circumventing the pitfalls associated with in vitro labeling. DC and lymphocytes have similar blood to afferent lymph migratory capabilities. This method has additional applications in tracking other rare cell populations in both normal and pathological states.