Veterinary Biosciences - Research Publications

Permanent URI for this collection

http://hdl.handle.net/11343/194095

Filters

1991 2
2
Reset filters

Settings
Statistics
Citations
Start date: 1990 × End date: 1999 × Author: Browning, Glenn ×

Search Results

Now showing 1 - 2 of 2
  • Item
    No Preview Available
    HOMOTYPIC AND HETEROTYPIC SERUM AND MILK ANTIBODY TO ROTAVIRUS IN NORMAL, INFECTED AND VACCINATED HORSES
    BROWNING, GF ; CHALMERS, RM ; SALE, CSH ; FITZGERALD, TA ; SNODGRASS, DR (ELSEVIER SCIENCE BV, 1991-05)
    The homotypic and heterotypic antibody response to rotavirus was determined in three pony mares and their foals. The normal concentrations of anti-rotavirus antibodies in mares' milk and mares' and foals' serum over the first 10 weeks post-partum were measured using IgA, IgG and rotavirus serotype-specific enzyme linked immunosorbent assays. Experimental infection of the foals with serotype 3 equine rotavirus produced a rapid, serotype-specific response which peaked 10 days after infection and a slower heterotypic response which peaked 32 days later. In contrast, vaccination of the mares with an inactivated, adjuvanted serotype 6 bovine rotavirus produced a heterotypic response similar to that of the homotypic response in both serum and milk, although the predominant response in serum was IgG, while in milk it was IgA. These results suggest that non serotype-restricted passive protection of foals against rotavirus may be achieved by parenteral vaccination of mares.
  • Item
    No Preview Available
    THE PREVALENCE OF ENTERIC PATHOGENS IN DIARRHEIC THOROUGHBRED FOALS IN BRITAIN AND IRELAND
    BROWNING, GF ; CHALMERS, RM ; SNODGRASS, DR ; BATT, RM ; HART, CA ; ORMAROD, SE ; LEADON, D ; STONEHAM, SJ ; ROSSDALE, PD (EQUINE VETERINARY JOURNAL LTD, 1991-11)
    A survey of 77 normal and 326 diarrhoeic foals in Britain and Ireland from 1987 to 1989 revealed a significantly higher prevalence of Group A rotaviruses and Aeromonas hydrophila in diarrhoeic foals. The prevalence of cryptosporidia, potentially pathogenic Escherichia coli, Yersinia enterocolitica and Clostridium perfringens was similar in normal or diarrhoeic foals. Rotaviruses had a similar prevalence in all age groups of scouring foals up to three months of age, with an overall prevalence of 37 per cent among diarrhoeic foals. The number of cases of diarrhoea varied considerably from year to year, but in all three years of the survey rotavirus was a significant pathogen. A comparison of diagnostic tests for rotavirus in the faeces showed electron microscopy (EM) and polyacrylamide gel electrophoresis (PAGE) to have similar sensitivity. The Rotazyme ELISA test kit was found to have the same sensitivity as a combination of EM and PAGE. A. hydrophila had an overall prevalence of 9 per cent among diarrhoeic foals, although its prevalence was higher in some age groups. A. hydrophila has not been established previously as a significant enteric pathogen in foals. Other putative pathogens found at very low prevalence were coronavirus, the putative picobirnavirus, Campylobacter spp. and Salmonella spp. No evidence was found of synergistic effects between rotavirus, cryptosporidia and potentially pathogenic E. coli. Neither coccidia nor non-Group A rotaviruses were found in any of the samples examined.