Science Collected Works - Research Publications

Permanent URI for this collection

http://hdl.handle.net/11343/303

Filters

2021 2
Reset filters

Settings
Statistics
Citations
Type: Journal Article × Author: O'Bryan, Moira × Author: Soria, Julio ×

Search Results

Now showing 1 - 2 of 2
  • Item
    Thumbnail Image
    CRISPs Function to Boost Sperm Power Output and Motility
    Gaikwad, AS ; Nandagiri, A ; Potter, DL ; Nosrati, R ; O'Connor, AE ; Jadhav, S ; Soria, J ; Prabhakar, R ; O'Bryan, MK (FRONTIERS MEDIA SA, 2021-08-05)
    Fertilization requires sperm to travel long distances through the complex environment of the female reproductive tract. Despite the strong association between poor motility and infertility, the kinetics of sperm tail movement and the role individual proteins play in this process is poorly understood. Here, we use a high spatiotemporal sperm imaging system and an analysis protocol to define the role of CRISPs in the mechanobiology of sperm function. Each of CRISP1, CRISP2, and CRISP4 is required to optimize sperm flagellum waveform. Each plays an autonomous role in defining beat frequency, flexibility, and power dissipation. We thus posit that the expansion of the CRISP family from one member in basal vertebrates, to three in most mammals, and four in numerous rodents, represents an example of neofunctionalization wherein proteins with a common core function, boosting power output, have evolved to optimize different aspects of sperm tail performance.
  • Item
    Thumbnail Image
    Flagellar energetics from high-resolution imaging of beating patterns in tethered mouse sperm
    Nandagiri, A ; Gaikwad, AS ; Potter, DL ; Nosrati, R ; Soria, J ; O'Bryan, MK ; Jadhav, S ; Prabhakar, R (ELIFE SCIENCES PUBLICATIONS LTD, 2021-04-30)
    We demonstrate a technique for investigating the energetics of flagella or cilia. We record the planar beating of tethered mouse sperm at high resolution. Beating waveforms are reconstructed using proper orthogonal decomposition of the centerline tangent-angle profiles. Energy conservation is employed to obtain the mechanical power exerted by the dynein motors from the observed kinematics. A large proportion of the mechanical power exerted by the dynein motors is dissipated internally by the motors themselves. There could also be significant dissipation within the passive structures of the flagellum. The total internal dissipation is considerably greater than the hydrodynamic dissipation in the aqueous medium outside. The net power input from the dynein motors in sperm from Crisp2-knockout mice is significantly smaller than in wildtype samples, indicating that ion-channel regulation by cysteine-rich secretory proteins controls energy flows powering the axoneme.