Medicine (Western Health) - Research Publications

Permanent URI for this collection

http://hdl.handle.net/11343/192934

Filters

2014 - 2018 3
Reset filters

Settings
Statistics
Citations
Author: Myers, Damian × Author: Ye, Kenneth × Start date: 2008 × Type: Journal Article ×

Search Results

Now showing 1 - 3 of 3
  • Item
    Thumbnail Image
    Osteochondral repair using an acellular dermal matrixpilot in vivo study in a rabbit osteochondral defect model
    Ye, K ; Traianedes, K ; Robins, SA ; Choong, PFM ; Myers, DE (WILEY, 2018-07)
  • Item
    Thumbnail Image
    Chondrogenesis of Infrapatellar Fat Pad Derived Adipose Stem Cells in 3D Printed Chitosan Scaffold
    Ye, K ; Felimban, R ; Traianedes, K ; Moulton, SE ; Wallace, GG ; Chung, J ; Quigley, A ; Choong, PFM ; Myers, DE ; Reilly, G (PUBLIC LIBRARY SCIENCE, 2014-06-11)
    Infrapatellar fat pad adipose stem cells (IPFP-ASCs) have been shown to harbor chondrogenic potential. When combined with 3D polymeric structures, the stem cells provide a source of stem cells to engineer 3D tissues for cartilage repair. In this study, we have shown human IPFP-ASCs seeded onto 3D printed chitosan scaffolds can undergo chondrogenesis using TGFβ3 and BMP6. By week 4, a pearlescent, cartilage-like matrix had formed that penetrated the top layers of the chitosan scaffold forming a 'cap' on the scaffold. Chondrocytic morphology showed typical cells encased in extracellular matrix which stained positively with toluidine blue. Immunohistochemistry demonstrated positive staining for collagen type II and cartilage proteoglycans, as well as collagen type I. Real time PCR analysis showed up-regulation of collagen type II, aggrecan and SOX9 genes when IPFP-ASCs were stimulated by TGFβ3 and BMP6. Thus, IPFP-ASCs can successfully undergo chondrogenesis using TGFβ3 and BMP6 and the cartilage-like tissue that forms on the surface of 3D-printed chitosan scaffold may prove useful as an osteochondral graft.
  • Item
    Thumbnail Image
    Chondrogenesis of Human Infrapatellar Fat Pad Stem Cells on Acellular Dermal Matrix
    Ye, K ; Traianedes, K ; Choong, PFM ; Myers, DE (FRONTIERS MEDIA SA, 2016-01-26)
    Acellular dermal matrix (ADM) has been in clinical use for decades in numerous surgical applications. The ability for ADM to promote cellular repopulation, revascularisation and tissue regeneration is well documented. Adipose stem cells have the ability to differentiate into mesenchymal tissue types, including bone and cartilage. The aim of this study was to investigate the potential interaction between ADM and adipose stem cells in vitro using TGFβ3 and BMP6. Human infrapatellar fat pad-derived adipose stem cells (IPFP-ASC) were cultured with ADM derived from rat dermis in chondrogenic (TGFβ3 and BMP6) medium in vitro for 2 and 4 weeks. Histology, qPCR, and immunohistochemistry were performed to assess for markers of chondrogenesis (collagen Type II, SOX9 and proteoglycans). At 4 weeks, cell-scaffold constructs displayed cellular changes consistent with chondrogenesis, with evidence of stratification of cell layers and development of a hyaline-like cartilage layer superficially, which stained positively for collagen Type II and proteoglycans. Significant cell-matrix interaction was seen between the cartilage layer and the ADM itself with seamless integration between each layer. Real time qPCR showed significantly increased COL2A1, SOX9, and ACAN gene expression over 4 weeks when compared to control. COL1A2 gene expression remained unchanged over 4 weeks. We believe that the principles that make ADM versatile and successful for tissue regeneration are applicable to cartilage regeneration. This study demonstrates in vitro the ability for IPFP-ASCs to undergo chondrogenesis, infiltrate, and interact with ADM. These outcomes serve as a platform for in vivo modelling of ADM for cartilage repair.