Genetics - Research Publications

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In vivo imaging and tracking of individual nanodiamonds in drosophila melanogaster embryos

Simpson, DA ; Thompson, AJ ; Kowarsky, M ; Zeeshan, NF ; Barson, MSJ ; Hall, LT ; Yan, Y ; Kaufmann, S ; Johnson, BC ; Ohshima, T ; Caruso, F ; Scholten, RE ; Saint, RB ; Murray, MJ ; Hollenberg, LCL (OPTICAL SOC AMER, 2014-04-01)

In this work, we incorporate and image individual fluorescent nanodiamonds in the powerful genetic model system Drosophila melanogaster. Fluorescence correlation spectroscopy and wide-field imaging techniques are applied to individual fluorescent nanodiamonds in blastoderm cells during stage 5 of development, up to a depth of 40 µm. The majority of nanodiamonds in the blastoderm cells during cellularization exhibit free diffusion with an average diffusion coefficient of (6 ± 3) × 10(-3) µm(2)/s, (mean ± SD). Driven motion in the blastoderm cells was also observed with an average velocity of 0.13 ± 0.10 µm/s (mean ± SD) µm/s and an average applied force of 0.07 ± 0.05 pN (mean ± SD). Nanodiamonds in the periplasm between the nuclei and yolk were also found to undergo free diffusion with a significantly larger diffusion coefficient of (63 ± 35) × 10(-3) µm(2)/s (mean ± SD). Driven motion in this region exhibited similar average velocities and applied forces compared to the blastoderm cells indicating the transport dynamics in the two cytoplasmic regions are analogous.