- Pharmacology and Therapeutics - Research Publications
Pharmacology and Therapeutics - Research Publications
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ItemSynthesis of Peptide Sequences Derived from Fibril-Forming ProteinsScanlon, DB ; Karas, JA ; Hill, AF ; Barnham, KJ ; Bottomley, SP ; Cappai, R (HUMANA PRESS INC, 2011)The pathogenesis of a large number of diseases, including Alzheimer's Disease, Parkinson's Disease, and Creutzfeldt-Jakob Disease (CJD), is associated with protein aggregation and the formation of amyloid, fibrillar deposits. Peptide fragments of amyloid-forming proteins have been found to form fibrils in their own right and have become important tools for unlocking the mechanism of amyloid fibril formation and the pathogenesis of amyloid diseases. The synthesis and purification of peptide sequences derived from amyloid fibril-forming proteins can be extremely challenging. The synthesis may not proceed well, generating a very low quality crude product which can be difficult to purify. Even clean crude peptides can be difficult to purify, as they are often insoluble or form fibrils rapidly in solution. This chapter presents methods to recognise and to overcome the difficulties associated with the synthesis, and purification of fibril-forming peptides, illustrating the points with three synthetic examples.
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ItemElucidating the Role of Metals in Alzheimer's Disease Through the Use of Surface-Enhanced Laser Desorption/Ionisation Time-of-Flight Mass SpectrometryWatt, AD ; Perez, KA ; Hung, LW ; Hill, AF ; Barnham, KJ ; Bottomley, SP ; Cappai, R (HUMANA PRESS INC, 2011)Alzheimer's disease (AD) is a highly heterogeneous and progressive dementia which is characterised by a progressive decline in cognitive functioning, selective neuronal atrophy, and loss of cortical volume in areas involved in learning and memory. However, recent research has indicated that the AD-affected brain is also besieged by increases in oxidative stress as well as perturbations to the homeostasis of biometals, such as copper and iron. These metals are known to interact with the neuropathological hallmark of AD, the β-amyloid peptide (Aβ), in a manner which increases Aβ's neurotoxic effects. This knowledge has led to the development of therapeutic measures which act to restore biometal homeostasis within the AD brain. This chapter outlines how Surface-Enhanced Laser Desorption/Ionisation Time-of-Flight Mass Spectrometry can be used to monitor Aβ levels within biological systems as well as describing the use of immobilised metal affinity capture in the observation of synthetic Aβ peptides.
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ItemPurinergic Receptors and Pain-An UpdateBurnstock, G ; AttaUrRahman, ; Choudhary, MI ; Reitz, AB (BENTHAM SCIENCE PUBL, 2016)
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ItemInsect envenomationWinkel, KD ; Senthilkumaran, S (Wolters Kluwer, 2012)