Microbiology & Immunology - Research Publications

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Subject: Medical Virology ; Infectious Diseases × Author: Kent, Stephen × Author: Fernandez, Caroline × Author: Brooks, Andrew ×

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    MHC class I allele frequencies in pigtail macaques of diverse origin
    Pratt, BF ; O'Connor, DH ; Lafont, BAP ; Mankowski, JL ; Fernandez, CS ; Triastuti, R ; Brooks, AG ; Kent, SJ ; Smith, MZ (SPRINGER, 2006-12)
    Pigtail macaques (Macaca nemestrina) are an increasingly common primate model for the study of human AIDS. Major Histocompatibility complex (MHC) class I-restricted CD8(+) T cell responses are a critical part of the adaptive immune response to HIV-1 in humans and simian immunodeficiency virus (SIV) in macaques; however, MHC class I alleles have not yet been comprehensively characterized in pigtail macaques. The frequencies of ten previously defined alleles (four Mane-A and six Mane-B) were investigated in detail in 109 pigtail macaques using reference strand-mediated conformational analysis (RSCA). The macaques were derived from three separate breeding colonies in the USA, Indonesia and Australia, and allele frequencies were analysed within and between these groups. Mane-A*10, an allele that restricts the immunodominant SIV Gag epitope KP9, was the most common allele, present in 32.1% of the animals overall, with similar frequencies across the three cohorts. Additionally, RSCA identified a new allele (Mane-A*17) common to three Indonesian pigtail macaques responding to the same Gag CD8(+) T cell epitope. This broad characterization of common MHC class I alleles in more than 100 pigtail macaques further develops this animal model for the study of virus-specific CD8(+) T cell responses.