School of BioSciences - Theses

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    Understanding the mechanism of the synthesis of β-D-(1,3;1,4)-glucans, or mixed linkage glucans (MLGs), by the cellulose synthase like (CSL) F6 protein
    Bain, Melissa ( 2018)
    The molecular mechanism by which mixed linkage, (1,3;1,4)-β-D-glucan, a non-cellulosic polysaccharide, is synthesised by cellulose synthase-like (CSL) F6 was explored. CSLF6 was shown to be crucial for development in the proposed model grass Brachypodium distachyon through analysis of point mutants. In silico homology modelling of related β-glucan synthases was used to predict domains influencing specificity, informing targeted mutagenesis. CSLF6 from Lolium multiflorum expressed in Escherichia coli was found to produce (1,3;1,4)-β-D-glucan similar to native sources in vitro, allowing biochemical characterisation.