Melbourne School of Population and Global Health - Research Publications

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Start date: 1985 × End date: 1989 × Author: Brown, Graham × Author: Culvenor, Janetta ×

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    Plasmodium falciparum: Cytoadherence of a knobless clone
    BIGGS, BA ; CULVENOR, JG ; NG, JS ; KEMP, DJ ; BROWN, GV (Elsevier, 1989-07)
    Sequestration of Plasmodium falciparum-infected erythrocytes is crucial to parasite survival as it prevents destruction in the liver and spleen. Knobs have been considered necessary but not sufficient for cytoadherence to vascular endothelial cells in vivo and to melanoma or umbilical vein endothelial cells in vitro. We describe here a knobless clone that cytoadheres strongly to C32 melanoma cells. This clone cannot express the knob-associated histidine-rich protein (KAHRP) due to the deletion of the KAHRP gene. Our results raise the possibility of an alternative mechanism for in vitro cytoadherence and suggest that the use of long term cultured isolates and melanoma cells as a model for cytoadherence in vivo may be misleading.
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    Localization of the ring-infected erythrocyte surface antigen (RESA) of Plasmodium falciparum in merozoites and ring-infected erythrocytes.
    Brown, GV ; Culvenor, JG ; Crewther, PE ; Bianco, AE ; Coppel, RL ; Saint, RB ; Stahl, HD ; Kemp, DJ ; Anders, RF (Rockefeller University Press, 1985-08-01)
    Immunoelectron microscopy with protein A gold has been used to determine the subcellular location of the ring-infected erythrocyte surface antigen (RESA) of Plasmodium falciparum. RESA was associated with dense vesicles presumed to be micronemes within merozoites. RESA was not detected on the surface of merozoites but was located at the membrane of erythrocytes infected with ring-stage parasites. RESA within merozoites was largely soluble in the nonionic detergent Triton X-100, but was insoluble in this detergent when associated with the erythrocyte membrane.