- School of Chemistry - Research Publications
School of Chemistry - Research Publications
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ItemNo Preview AvailableImmobilization and Intracellular Delivery of an Anticancer Drug Using Mussel-Inspired Polydopamine CapsulesCui, J ; Yan, Y ; Such, GK ; Liang, K ; Ochs, CJ ; Postma, A ; Caruso, F (AMER CHEMICAL SOC, 2012-08)We report a facile approach to immobilize pH-cleavable polymer-drug conjugates in mussel-inspired polydopamine (PDA) capsules for intracellular drug delivery. Our design takes advantage of the facile PDA coating to form capsules, the chemical reactivity of PDA films, and the acid-labile groups in polymer side chains for sustained pH-induced drug release. The anticancer drug doxorubicin (Dox) was conjugated to thiolated poly(methacrylic acid) (PMA(SH)) with a pH-cleavable hydrazone bond, and then immobilized in PDA capsules via robust thiol-catechol reactions between the polymer-drug conjugate and capsule walls. The loaded Dox showed limited release at physiological pH but significant release (over 85%) at endosomal/lysosomal pH. Cell viability assays showed that Dox-loaded PDA capsules enhanced the efficacy of eradicating HeLa cancer cells compared with free drug under the same assay conditions. The reported method provides a new platform for the application of stimuli-responsive PDA capsules as drug delivery systems.
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ItemNo Preview AvailablePhotoinitiated Alkyne-Azide Click and Radical Cross-Linking Reactions for the Patterning of PEG HydrogelsChen, RT ; Marchesan, S ; Evans, RA ; Styan, KE ; Such, GK ; Postma, A ; McLean, KM ; Muir, BW ; Caruso, F (AMER CHEMICAL SOC, 2012-03)The photolithographical patterning of hydrogels based solely on the surface immobilization and cross-linking of alkyne-functionalized poly(ethylene glycol) (PEG-tetraalkyne) is described. Photogenerated radicals as well as UV absorption by a copper chelating ligand result in the photochemical redox reduction of Cu(II) to Cu(I). This catalyzes the alkyne-azide click reaction to graft the hydrogels onto an azide-functionalized plasma polymer (N(3)PP) film. The photogenerated radicals were also able to abstract hydrogen atoms from PEG-tetraalkyne to form poly(α-alkoxy) radicals. These radicals can initiate cross-linking by addition to the alkynes and intermolecular recombination to form the PEG hydrogels. Spatially controlling the two photoinitiated reactions by UV exposure through a photomask leads to surface patterned hydrogels, with thicknesses that were tunable from tens to several hundreds of nanometers. The patterned PEG hydrogels (ca. 60 μm wide lines) were capable of resisting the attachment of L929 mouse fibroblast cells, resulting in surfaces with spatially controlled cell attachment. The patterned hydrogel surface also demonstrated spatially resolved chemical functionality, as postsynthetic modification of the hydrogels was successfully carried out with azide-functionalized fluorescent dyes via subsequent alkyne-azide click reactions.
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ItemEngineering Particles for Therapeutic Delivery: Prospects and ChallengesYan, Y ; Such, GK ; Johnston, APR ; Best, JP ; Caruso, F (AMER CHEMICAL SOC, 2012-05)Nanoengineered particles that can facilitate drug formulation and passively target tumors have reached the clinic in recent years. These early successes have driven a new wave of significant innovation in the generation of advanced particles. Recent developments in enabling technologies and chemistries have led to control over key particle properties, including surface functionality, size, shape, and rigidity. Combining these advances with the rapid developments in the discovery of many disease-related characteristics now offers new opportunities for improving particle specificity for targeted therapy. In this Perspective, we summarize recent progress in particle-based therapeutic delivery and discuss important concepts in particle design and biological barriers for developing the next generation of particles.
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ItemTargeting Cancer Cells: Controlling the Binding and Internalization of Antibody-Functionalized CapsulesJohnston, APR ; Kamphuis, MMJ ; Such, GK ; Scott, AM ; Nice, EC ; Heath, JK ; Caruso, F (AMER CHEMICAL SOC, 2012-08)The development of nanoengineered particles, such as polymersomes, liposomes, and polymer capsules, has the potential to offer significant advances in vaccine and cancer therapy. However, the effectiveness of these carriers has the potential to be greatly improved if they can be specifically delivered to target cells. We describe a general method for functionalizing nanoengineered polymer capsules with antibodies using click chemistry and investigate their interaction with cancer cells in vitro. The binding efficiency to cells was found to be dependent on both the capsule-to-cell ratio and the density of antibody on the capsule surface. In mixed cell populations, more than 90% of target cells bound capsules when the capsule-to-target cell ratio was 1:1. Strikingly, greater than 50% of target cells exhibited capsules on the cell surface even when the target cells were present as less than 0.1% of the total cell population. Imaging flow cytometry was used to quantify the internalization of the capsules, and the target cells were found to internalize capsules efficiently. However, the role of the antibody in this process was determined to enhance accumulation of capsules on the cell surface rather than promote endocytosis. This represents a significant finding, as this is the first study into the role antibodies play in internalization of such capsules. It also opens up the possibility of targeting these capsules to cancer cells using targeting molecules that do not trigger an endocytic pathway. We envisage that this approach will be generally applicable to the specific targeting of a variety of nanoengineered materials to cells.
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ItemNo Preview AvailableSynthesis and functionalization of nanoengineered materials using click chemistrySuch, GK ; Johnston, APR ; Liang, K ; Caruso, F (PERGAMON-ELSEVIER SCIENCE LTD, 2012-07)
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ItemNo Preview AvailableClick poly(ethylene glycol) multilayers on RO membranes: Fouling reduction and membrane characterizationWang, C ; Such, GK ; Widjaya, A ; Lomas, H ; Stevens, G ; Caruso, F ; Kentish, SE (ELSEVIER SCIENCE BV, 2012-08-01)