School of Physics - Research Publications
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ItemNo Preview AvailableSpeckle and Conservation(International Seminars, 2008)The aim of this research is to demonstrate the usefulness of speckle, a trait of an expanded laser beam, for the non-destructive testing of artwork in both the imaging of subsurface structure and the quantitative detection of physical movement of canvas. Laser Speckle Contrast Method (LSCI) is a useful method for the viewing of subsurface layers and movement. By investigating the statistical properties of dynamic speckle it is possible to reveal drawings that are hidden beneath scattering layers such as the primary layer of paint or adhered paper. This is achieved by taking a series of speckle images captured in a short time frame and applying one of a number of post processing algorithms. We explore the limitations of this method when applied to various paper samples that have a sketch executed in various media beneath the top layer. The ability to resolve gray scale images was examined as well as looking at the dependence of the contrast of the revealed drawings to the temperature of the surface. Current work is being done on using LSCI to reveal indentations in artwork caused by the application process. The successful use of Electronic Speckle Pattern Interferometry (ESPI) both in the laboratory and in-situ for the detection of in-plane movement of painted canvas due to humidity fluctuations and the out-of-plane movement of paint as it dries has also been demonstrated. Canvas paintings can be very susceptible to movement due to changes of the environment. ESPI is a non-destructive technique yielding sensitive results that can detect displacement on a surface of less than the wavelength of the illuminating coherent light source. While ESPI has been successfully applied to the in-situ study of painted frescoes, previous studies have employed tensile testers as a support for painted canvas. We have shown a portable version of ESPI to be of use in tropical environment in the Philippines, Malaysia and Singapore with original artworks where variations in humidity occur and the samples have not undergone special preparation before analysis, revealing significant directional movements. Furthermore, a simple variation in the direction of beams paths permits the characterisation of out-of-plane movement, specifically as the height of paint shrinks due to the drying process. We have used ESPI to view the drying process of alkyd resin paints over the time period of 24 hours.
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ItemGene Regulation in Primates Evolves under Tissue-Specific Selection Pressures(PUBLIC LIBRARY SCIENCE, 2008-11)Regulatory changes have long been hypothesized to play an important role in primate evolution. To identify adaptive regulatory changes in humans, we performed a genome-wide survey for genes in which regulation has likely evolved under natural selection. To do so, we used a multi-species microarray to measure gene expression levels in livers, kidneys, and hearts from six humans, chimpanzees, and rhesus macaques. This comparative gene expression data allowed us to identify a large number of genes, as well as specific pathways, whose inter-species expression profiles are consistent with the action of stabilizing or directional selection on gene regulation. Among the latter set, we found an enrichment of genes involved in metabolic pathways, consistent with the hypothesis that shifts in diet underlie many regulatory adaptations in humans. In addition, we found evidence for tissue-specific selection pressures, as well as lower rates of protein evolution for genes in which regulation evolves under natural selection. These observations are consistent with the notion that adaptive circumscribed changes in gene regulation have fewer deleterious pleiotropic effects compared with changes at the protein sequence level.
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ItemA Combination of Genomic Approaches Reveals the Role of FOXO1a in Regulating an Oxidative Stress Response Pathway(PUBLIC LIBRARY SCIENCE, 2008-02-27)BACKGROUND: While many of the phenotypic differences between human and chimpanzee may result from changes in gene regulation, only a handful of functionally important regulatory differences are currently known. As a first step towards identifying transcriptional pathways that have been remodeled in the human lineage, we focused on a transcription factor, FOXO1a, which we had previously found to be up-regulated in the human liver compared to that of three other primate species. We concentrated on this gene because of its known role in the regulation of metabolism and in longevity. METHODOLOGY: Using a combination of expression profiling following siRNA knockdown and chromatin immunoprecipitation in a human liver cell line, we identified eight novel direct transcriptional targets of FOXO1a. This set includes the gene for thioredoxin-interacting protein (TXNIP), the expression of which is directly repressed by FOXO1a. The thioredoxin-interacting protein is known to inhibit the reducing activity of thioredoxin (TRX), thereby hindering the cellular response to oxidative stress and affecting life span. CONCLUSIONS: Our results provide an explanation for the repeated observations that differences in the regulation of FOXO transcription factors affect longevity. Moreover, we found that TXNIP is down-regulated in human compared to chimpanzee, consistent with the up-regulation of its direct repressor FOXO1a in humans, and with differences in longevity between the two species.
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ItemA preliminary understanding of oil paintings in tropical Southeast Asia(Allied Publishers Pvt. Ltd., 2008)
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ItemThe Early History of Monash University's Men's Cricket Club The First Decade (Season 1962/63 - Season 1971/72)( 2008)"The book documents the development of cricket at Monash University and the University Cricket Club, from the opening of the university (Clayton Campus).
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ItemNo Preview AvailableCollisions of bright solitary matter waves(IOP PUBLISHING LTD, 2008-02-28)
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ItemSpatial coherent transport of interacting dilute Bose gases(AMER PHYSICAL SOC, 2008-06)
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ItemCollapse times of dipolar Bose-Einstein condensates(AMER PHYSICAL SOC, 2008-12)
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ItemBright solitary waves of atomic Bose-Einstein condensates under rotation(AMER PHYSICAL SOC, 2008-05)