Melbourne Dental School - Research Publications

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    Status and progress of treatment methods for root caries in the last decade: a literature review
    Cai, J ; Palamara, JEA ; Manton, DJ ; Burrow, MF (WILEY, 2018-03)
    The aim of this literature review is to explore the treatment methods for root caries in laboratory and clinical research in the last decade. A systematic search of publications in PubMed and Web of Science databases was performed. The timespan was limited to the last 10 years and English language. Further retrieval was conducted using the search terms of specific therapies or treatments. Eighty-two articles were included in this systematic review and full texts were retrieved. Types of studies included laboratory studies and clinical trials. Therapeutic approaches for root caries without risk of pulp exposure can be categorized into non-invasive and restorative treatment. Non-invasive treatments which targeted different causative factors of root caries have been developed in the last decade. Accordingly, several artificial caries model systems have been proposed for the study of root caries in the laboratory. Carious tissue excavation techniques and restorative materials and procedures have been modified to improve the prognosis of invasive treatment. It is of importance to determine the most appropriate therapy for root caries and further clinical trials are needed to draw firm conclusions concerning the efficacy and consistency of the various treatment methods proposed.
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    Effect of a self-adhesive coating on the load-bearing capacity of tooth-coloured restorative materials
    Bagheri, R ; Palamara, JEA ; Mese, A ; Manton, DJ (WILEY, 2017-03)
    BACKGROUND: The aim of this study was to compare the flexural strength and Vickers hardness of tooth-coloured restorative materials with and without applying a self-adhesive coating for up to 6 months. METHODS: Specimens were prepared from three resin composites (RC), two resin-modified glass-ionomer cements (RM-GIC) and two conventional glass-ionomer cements (CGIC). All materials were tested both with and without applying G-Coat Plus (GCP). Specimens were conditioned in 37 °C distilled deionized water for 24 h, and 1, 3 and 6 months. The specimens were strength tested using a four-point bend test jig in a universal testing machine. The broken specimen's halves were used for Vickers hardness testing. Representative specimens were examined under an environmental scanning electron microscope. RESULTS: Data analysis showed that regardless of time and materials, generally the surface coating was associated with a significant increase in the flexural strength of the materials. Applying the GCP decreased the hardness of almost all materials significantly (P < 0.05) and effect of time intervals on hardness was material dependent. CONCLUSIONS: The load-bearing capacity of the restorative materials was affected by applying self-adhesive coating and ageing. The CGIC had significantly higher hardness but lower flexural strength than the RM-GIC and RC.
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    An investigation into the effect of a resin infiltrant on the micromechanical properties of hypomineralised enamel
    Kumar, H ; Palamara, JEA ; Burrow, MF ; Manton, DJ (WILEY, 2017-09)
    BACKGROUND: Resin infiltration may alter the mechanical properties of enamel hypomineralised lesions (HL); however, variable surface layer (SL) thickness may affect resin penetration. AIMS: To determine the thickness of the SL of HL and to investigate the effect of resin infiltration on the mechanical properties of HL. DESIGN: The thickness of the SL over HL was determined using polarised light microscopy. Etching time using 15% HCl gel to remove the SL of 52 samples was determined. Selected HL and control areas of 21 teeth were infiltrated with Icon® resin infiltrant, and cross-sectional Knoop microhardness (KHN) measurements were recorded before and after resin infiltration. RESULTS: Ninety-six point five per cent of HL had a detectable SL with mean thickness of 58 ± 29 μm. Application of HCl for 120 s produced a mean MIH erosion depth of 58 ± 12 μm. Eleven of 21 samples had evidence of infiltration using visual examination. The infiltrant penetrated some of the HL leading to an increase in KHN (111 ± 75 KHN) which, when compared to adjacent non-infiltrated HL (96 ± 52 KHN), was not statistically significantly different (P = 0.56). CONCLUSIONS: There was marked variation in the SL thickness of HL. Resin infiltration of HL did not increase microhardness significantly.
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    Characterisation of developmentally hypomineralised human enamel
    Crombie, FA ; Manton, DJ ; Palamara, JEA ; Zalizniak, I ; Cochrane, NJ ; Reynolds, EC (ELSEVIER SCI LTD, 2013-07)
    OBJECTIVES: To investigate and clarify physical and chemical properties of enamel affected by molar incisor hypomineralisation (MIH). METHODS: A series of in vitro studies were performed on extracted molars affected by MIH and sound teeth for controls. Tooth sections underwent Vickers microhardness testing before lapping and subsequent transverse microradiographic analysis and examination under polarised light microscopy. Carbonate content was determined by CO2 release from acid digestion. Unprepared and fractured surfaces were examined under scanning electron microscopy. RESULTS: MIH-affected molars demonstrated a severe degree of hypomineralisation with an average mineral content of only 58.8%vol% mineral. Vickers microhardness was significantly reduced in MIH compared with controls (1.8±1.1 v 4.4±1.0 GPa, p<0.05) and polarised light microscopy revealed the bulk of MIH lesions had a porosity of ≤5% but also substantial areas of ≥10% and smaller areas exceeding 25% porosity. A surface layer was frequently observed on both intact and broken-down lesions and cervical regions of MIH teeth were typically spared. Carbonate content of MIH enamel was higher than control samples (6.6±2.1 v 4.4±1.1 wt%, p<0.05). Scanning electron microscopy showed that both the enamel rod and surface ultrastructure were defective. Clinical characteristics did not consistently correlate with all properties. CONCLUSIONS: The properties of MIH-affected enamel significantly differ from those of normal enamel and were highly variable, however some common characteristics were observed. Implications for aetiology and clinical management are discussed.