Microbiology & Immunology - Theses

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The development of attenuated Salmonella typhimurium as a vaccine vector

Dunstan, Sarah Jane ( 1997)

Investigation of attenuated Salmonella typhimurium vaccine vectors in the murine model is an important step in the development of new and effective multivalent S. typhi vaccines. This thesis examined a number of parameters involved in the effective delivery of heterologous antigens from live attenuated S. typhimurium. S. Typhimurium was engineered to express the model heterologous antigen C fragment and the affects of (i) the type of attenuating mutation the S. Typhimurium harbours, (ii) the type of promoter used to direct expression of the heterologous antigen, and (iii) the type of plasmid used to encode the heterologous antigen, were examined. In addition, the immunobiological consequences of encoding murine interleukin-6 (mIL-6) in S. Typhimurium were investigated. Information obtained from optimisation studies was used to construct a novel S. Typhimurium/rotavirus vaccine, which was evaluated in the murine model. (From Summary)