Clinical Pathology - Research Publications

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Author: George, Amee × Author: Kedzierska, Katherine × End date: 2022 × Start date: 2020 ×

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    A Dual-Antigen Enzyme-Linked Immunosorbent Assay Allows the Assessment of Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Seroprevalence in a Low-Transmission Setting
    Hicks, SM ; Pohl, K ; Neeman, T ; McNamara, HA ; Parsons, KM ; He, J-S ; Ali, SA ; Nazir, S ; Rowntree, LC ; Nguyen, THO ; Kedzierska, K ; Doolan, DL ; Vinuesa, CG ; Cook, MC ; Coatsworth, N ; Myles, PS ; Kurth, F ; Sander, LE ; Mann, GJ ; Gruen, RL ; George, AJ ; Gardiner, EE ; Cockburn, IA (OXFORD UNIV PRESS INC, 2021-01-01)
    Estimates of seroprevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies have been hampered by inadequate assay sensitivity and specificity. Using an enzyme-linked immunosorbent assay-based approach that combines data about immunoglobulin G responses to both the nucleocapsid and spike receptor binding domain antigens, we show that excellent sensitivity and specificity can be achieved. We used this assay to assess the frequency of virus-specific antibodies in a cohort of elective surgery patients in Australia and estimated seroprevalence in Australia to be 0.28% (95% Confidence Interval, 0-1.15%). These data confirm the low level of transmission of SARS-CoV-2 in Australia before July 2020 and validate the specificity of our assay.
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    A dual antigen ELISA allows the assessment of SARS-CoV-2 antibody seroprevalence in a low transmission setting
    Hicks, S ; Pohl, K ; Neeman, T ; McNamara, H ; Parsons, K ; He, J-S ; Ali, S ; Nazir, S ; Rowntree, L ; Nguyen, T ; Kedzierska, K ; Doolan, D ; Vinueas, C ; Cook, M ; Coatsworth, N ; Myles, P ; Kurth, F ; Sander, L ; Mann, G ; Gruen, R ; George, A ; Gardiner, E ; Cockburn, I ; the SARS-CoV-2 Testing in Elective Surgery Collaborators, (Cold Spring Harbor Laboratory, 2020)
    Estimates of seroprevalence of SARS-CoV-2 antibodies have been hampered by inadequate assay sensitivity and specificity. Using an ELISA-based approach to that combines data about IgG responses to both the Nucleocapsid and Spike-receptor binding domain antigens, we show that near-optimal sensitivity and specificity can be achieved. We used this assay to assess the frequency of virus-specific antibodies in a cohort of elective surgery patients in Australia and estimated seroprevalence in Australia to be 0.28% (0 to 0.72%). These data confirm the low level of transmission of SARS-CoV-2 in Australia before July 2020 and validate the specificity of our assay.