School of Agriculture, Food and Ecosystem Sciences - Theses
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ItemEstimation of the available amino acid contents of feeds for pigs with Tetrahymena pyriformis w( 1973)Experiments were conducted to evaluate the usefulness of the Tetrahymena assay for predicting the available amino acid content of feeds for pigs. In collaborative studies the Tetrahymena lysine values were also compared with values from the Silcock technique for a series of feeds and attempts were made to evaluate which technique was more applicable to pigs. The pig experiments were conducted with pigs fed individually once daily during the 20 to 45 kg growth phase. Pig response was assessed in terms of live-weight gain, feed conversion efficiency and carcass lean as estimated by joint dissections. 1) The 4-day incubation period for the assay of proteins by Tetrahymena as recommended by Stott and. Smith (1966) was found to be adequate for buttermilk powder but not for fish meal or meat and bone meal. By extending the incubation of the latter two meals to 7 days, complete hydrolysis was achieved. Experiments with pigs fed diets formulated with values from either the 4-day or the 7.-day incubation periods for fish meal and meat and bone meal indicated that values obtained with the Tetrahymena assay after complete hydrolysis of the meals were more applicable to pigs than were those estimated after 4-days. 2) For cereals a 10-day incubation period for estimates of lysine and tryptophan with Tetrahymena was found necessary for maximum hydrolysis, and there was good agreement between these values and the response in pigs. 3) There was close agreement between Tetrahymena and Silcock estimates for lysine in samples of soyabean meal that were autoclaved for varying times. In a pig experiment the additions of lysine, methionine and tryptophan to diets containing the autoclaved soyabean meal restored only 43% of the effects of the heat damage. 4) This raised the question as to whether the supplements of free amino acids had been efficiently utilised by the pigs. A pig experiment was conducted to examine the effect of feeding a ration containing free lysine either once daily or in six equal portions at three hourly intervals. The results indicated that only 43% of the free lysine was utilised with once daily feeding relative to the frequent feeding regime. Thus the inability of the amino acids added to the autoclaved soyabean meal to compensate for the effects of heat damage may have been due to inefficient utilisation of the added amino ' acids by the pigs and not to inapplicability of the estimates of heat damage. 5) The amount of protein used in the Tetrahymena assay was found to govern the subsequent hydrolysis of the protein. Attempts to promote more vigourous hydrolysis of small amounts of protein by increasing the amount of inoculum were unsuccessful. Vigourous hydrolysis of the protein was obtained by the addition of 0.25 mg L-lysine-HC1 to each assay; this stimulated the initial growth of Tetrahymena, then vigourous hydrolysis resulted. 6) The rate of release of lysine from protein concentrates by Tetrahymena varied. Buttermilk powder and skim milk powder required 2 days, peanut meal 3 days, soyabean meal 4 days, safflower meal 5 days, rapeseed meal 6 days and fishmeal and meat meal 7 to 8 days. Free lysine was utilised within 4 days. 7) The Tetrahymena and Silcock lysine values for different protein concentrates showed no consistent pattern. For soyabean meal the Tetrahymena lysine values were greater than the Silcock values, for peanut meal the estimates were similar, and for the other proteins the Tetrahymena values were lower than the Silcock values. This was particularly so for a meat meal and rapeseed meal where the Tetrahymena values were approximately half the Silcock estimates. 8) An attempt was made to supplement cereal-based diets with protein-bound lysine to achieve diets of similar lysine content; both the Tetrahymena and the Silcock values were used to compute the amount of supplementary protein needed. Diets formulated according to these two values were then fed to pigs in an attempt to decide which value was the better indicator of lysine availability to pigs. This experiment proved inconclusive as factors other than lysine appeared to have influenced the results. This aspect of the comparison of the two methods of assay needs to be examined using feeding trials on a larger scale to eliminate these complicating factors. 9) In Appendix 1, the modifications made to the Tetrahymena assay to allow complete hydrolysis of proteins are Outlined.