School of Agriculture, Food and Ecosystem Sciences - Theses
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ItemEpidemiological and physiological studies of the effects of peach rosette and decline disease on the peach, prunus persicae L. Batsch( 1975)The incidence in the field of the disease peach rosette and decline (PRD), which is of considerable economic importance in the Goulburn Valley, causing fruit loss and tree death, was shown to increase from 0.9 to 91.3% in an orchard of cv. Golden Queen in 10 years. Similar results were found with the cv. Pullars Cling, in which infection increased from 1.5 to 29.7% over five years. The pattern of spread was mainly from infected trees to contiguous uninfected trees. This is consistent with the view that the main causal agent, prune dwarf virus (PDV), is transmitted only via the transfer of infected pollen : a previous finding in cherries which was confirmed in peaches. Prunus necrotic ringspot virus (PNRV) is the other virus always present in the field in PRD-infected trees. The mode of spread of PNRV is also by pollen. Within the tree, PDV moved erratically from the first infected limb, via phloem but not xylem, into the other limbs well in advance of the appearance of symptoms. Three months after flowering, PDV was detected in 65% of main limbs adjacent to the first infected limbs but in only 30% of limbs more remotely positioned on the tree. However, removing infected limbs within four weeks of flowering, when the initial infection was presumed to have occurred, did not prevent the movement of PDV into the rest of the tree. Laterals from peach trees infected with PRD were tested for the presence of PDV, using woody virus indicators (cvs Golden Queen, Italian Prune and Elberta seedling). Golden Queen was found to be a more reliable indicator for detecting PDV than Italian prune, as the presence of PNRV with PDV killed 71% of the Italian prune buds compared to only 34% of the Golden Queen buds. Golden Queen also developed more obvious foliage symptoms of PDV infection than Elberta seedlings. The probability of failing to detect PDV in infected field trees, using all three indicator plants, was higher in the first year of infection. The rate of spread of PRD was reduced in the orchard by preventing infected trees from flowering, either by removing obviously infected trees or by deblossoming. Removing infected trees resulted in a three-fold reduction in the spread of the disease in two seasons. Removing the flowers from infected trees before pollination reduced the spread of the disease by about half. This, only partial, control of the spread of PRD by tree removal or deblossoming was attributed to the presence of up to 14.3% of trees without symptoms being latently infected with PDV. It was observed that deliberate infection with PDV by pollen also resulted in a slow expression of the symptoms of PRD. The effects of PRD on the growth of young peach trees was obvious in the first three months of growth. There were considerable varietal differences in the severity of this effect. Those varieties based on cvs. Golden Queen or Levis Cling were more severely affected than the variety Elberta. The results from shoot elongation measurements agreed with those obtained from conventional growth analysis methods. These latter experiments showed that, after three months, the dry weight and leaf area of infected Golden Queen plants were reduced by 94%. The fruit yield from mature PRD-free trees was three times that of trees infected for the first season, even though symptoms were apparent only on one limb; and six times that from chronically affected trees infected for two seasons. The effect of virus infection on the photosynthetic ability of single, attached peach leaves was studied under laboratory conditions using infra red gas analysis. The constants derived from the equations describing the relationship between net photosynthesis (Pn) and both irradiance and CO2 concentration were used to analyse the effects of infection by PRD on photosynthetic characteristics of the leaf. The asymptotic value of Pn (Pmax) in young leaves was reduced 15% by PRD-infection, mainly through an increase in the "residual resistance" to 002 diffusion and a decrease of 23% in the parameter indicating photochemical efficiency. There was also evidence that the gas phase resistance was higher in infected leaves at low levels of irradiance. Dark respiration was 51% higher in infected leaves, but this difference was not significant. PRD did not reduce Pn in 60-day-old leaves, normal leaf senescence having a predominant and greater effect. It was concluded that PRD infection had its large effects on growth via a reduction in leaf area; the effects on the photosynthetic capacity per unit leaf area being minor. An effect of PRD infection on the translocation of 14C-assimilatesout of leaves was also observed. Infected leaves retained twice the assimilates than did uninfected leaves. It is concluded that the most promising methods of control of PRD include removal of infected trees, deblossoming suspected infected trees until diagnosis is confirmed, use of virus-tested plants, the gradual destruction of infected orchards and protecting young, healthy orchards from infection either by barrier crops or deblossoming the young plants until they reach an economic bearing age.
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ItemThe ecology and physiology of two species of Carduus as weeds of pastures in Victoria( 1977)Slender thistles (Carduus pycnocephalus and C. tenuiflorus) were introduced to Australia about the 1880s. They are now important weeds of pastures in much of southern Australia and are difficult to control with present methods. This study was undertaken to investigate several aspects of the ecology and physiology of the plants with the belief that a knowledge of some of these aspects, particularly of seed germination and seedling establishment, might disclose some "weakness" in the plants' growth which could be exploited to improve control measures. Because of confusion over differences between the two species which occur in Australia the initial step was to evaluate the morphological features which have been used to distinguish between the two species. Although they are very similar morphologically, cytological evidence confirmed that the two species were quite distinct and, in fact, had quite different evolutionary origins. Germination of seeds of slender thistles is controlled by three separate forms of dormancy; these are known as innate, induced and enforced dormancy. Dormancy ensures that the plants will survive in a Mediterranean-type climate and also colonize areas with quite different climates and, most importantly, survive natural catastrophes such as drought, fire, and flood. The germination of slender thistles in the field is confined to a very short period (about 6 weeks) after the autumn break in any year. This is a "weakness" in the plants' survival mechanism because they are vulnerable in that year, at least, to any treatment which can kill seedlings. The herbicide, diquat, was found to kill young seedlings of slender thistles and not affect seedlings of desirable pasture plants associated with the thistles in southern Australia. This treatment is economical and leads not only to a reduction in thistles but an increase of about 30% in pasture production. Several other aspects of the plants' growth were investigated. Slender thistles have early growth characters which give them advantages over more desirable components of pastures. They are more competitive than subterranean clover and ryegrass over a wide range of levels of nutrients, and the traditional approach to pasture improvement in southern Australia of applying superphosphate and sowing subterranean clover will encourage, not suppress, slender thistles. Since grazing animals generally do not eat slender thistles the presence of thistles in pastures at densities commonly occurring in Victoria considerably reduces pasture production.
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ItemThe occurrence of brittleheart in Eucalyptus regnans and its effect on various wood properties( 1990)This project is mainly concerned with a description of anatomical and ultrastructural characteristics of cell wall deformations in brittleheart of E. regnans, development of methodology for quantification of percentage of broken fibre pieces (PBFP) in E. regnans, and physical and anatomical properties of E. regnans in relation to the occurrence of brittleheart. Two 1939 regrowth E. regnans butt logs and one mature growth E. regnans butt log removed from a tree approximately 120 years old were used in the study. The anatomical and ultrastructural characteristics of cell wall deformations were examined with bright field, polarized light, and scanning electron microscopy. The microscopic compression lines were found to consist of slip planes, minute compression failures, corrugations, and buckles. The width of microscopic compression lines along the longitudinal fibre axis ranged from one single fold in the cell wall up to 7 times the average fibre width. The length of the microscopic compression lines ranged from ones which only crossed a few fibres to ones which crossed up to 200 fibres. The severity of microscopic compression lines appeared to be dependent on the type of cell wall deformations and degree of compaction in the deformed zone. In the methodology studies it was found that for a pulp sample prepared from a 2 mm by 2 mm by 15 mm wood chip approximately 400 cellular elements in 8 out of 16 areas on a slide needed to be counted in order to obtain PBFP with less than 10% error. For macerated thin sections, all the cellular elements on 2 slides each carrying about 600 fibres and fibre pieces needed to be counted. A highly significant correlation was found between the length of microscopic compression lines per unit area and the microscopically determined PBFP. A highly significant correlation was found between the theoretical PBFP and the microscopically determined PBFP. These findings confirm that maceration of wood containing cell wall deformations results in broken fibres and verifies the validity of the maceration technique for quantifying the amount of microscopic compression lines. PBFP was found to increase with macerating time. A maceration time around 10 hours appears sufficient to cause fibres having cell wall deformations to break but longer times cause fibres without cell wall deformations to also break due to overmaceration. A significant relationship was found between PBFP determined after 5 hours and after 10 hours of maceration at the same temperature. It was found that parameters extracted or derived from cell length distributions produced by the Kajaani FS-200 may be used to determine the amount of fibre fragments in pulp samples. These parameters were the high peak, the length weighted average, and the mass weighted average of the cell length distributions for pulps which had PBFP greater than 10. Based on the maceration technique, brittleheart was detected in both the mature and the 1939 regrowth logs. Brittleheart was more severe and occupied a larger area in the mature growth than in the regrowth wood. The PBFP was found to be mostly below 5 in the regrowth wood although relatively high PBFP values of 21 and 30 were observed. PBFP values as high as 85 was found in the mature growth wood. In general, PBFP was found to be higher nearer the pith, decreasing toward the bark, and dropping to zero before reaching the two-thirds theoretical point where the stress is assumed to be 0. A large circumferential variation in PBFP was observed in four adjacent growth rings of the mature log. A large variation in PBFP was also found within a volume of 1000 mm^3 for both the mature and the 1939 regrowth wood. The earlywood PBFP was found to be significantly higher than the latewood PBFP for the mature and regrowth logs. A total of 72 green and 132 12% MC Izod specimens were prepared from the two 1939 regrowth logs and tested for impact strength. The mean Izod value was found to be 9.9 ft.lb for the 132 12% MC Izod specimens and 9.2 ft.lb for the 72 green Izod specimens. For side-matched Izod specimens, the mean Izod value was found to be 9.2 ft.lb at green and 9.7 ft.lb at 12% MC and the mean for the 12% MC specimens did not reflect the expected increase in strength with moisture loss. Excessively low Izod values (eg. 1.8 ft.Ib) were found in the 12% MC Izod specimens located near the pith. For 76 12% MC Izod specimens, their PBFP, fibre length, and specific gravity were also measured. Significant relationships (p=0.01) were found between Izod values and specific gravity, PBFP, and fibre length for these 76 12% MC Izod specimens. Specific gravity and fibre length positively contribute to the impact strength whereas PBFP negatively affects the impact strength. Brash-break specimens showed a low mean Izod value, a low mean specific gravity, the presence of brittleheart, and a short mean fibre length.
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ItemThe activity of glyphosate and other herbicides in soil( 1989)The effects of herbicides on a legume Rhizobium symbiosis were studied in laboratory experiments. Root applications of all herbicides examined reduced nodulation of legumes grown in aqueous nutrient media. The growth of Rhizobium trifolii TA1 was reduced by 2 - 20 mg ai 1-1 of diquat, 2 mg ai 1-1 of paraquat, 10 mg ai 14 of glyphosate and 2 mg ail-1 of chlorsulfuron. No other herbicide affected rhizobia growth when applied at 2 - 20 mg ai l-1 of nutrient medium. Inoculation with TA1 pre-treated with amitrole, atrazine or glyphosate decreased nodulation of sub-clover plants indicating that these herbicides may affect the nodulation potential of certain strains of Rhizobium. Residues of 2,4-D, amitrole, diquat, trifluralin and glyphosate persisted in a Walpeup sandy loam in sufficient concentration for four months after application to soil to affect growth and symbiotic activity of sub-clover. The behaviour of glyphosate in soil, under various conditions was studied in the laboratory. Adsorption of glyphosate as depicted by Freundlich K constant was greater in an acid soil than in three alkaline soils and values for this constant ranged from 8 - 67.8 at 23.5C and 4.3 - 57.8 at 10C. Rate of decomposition of 14C-glyphosate at 25C decreased slowly over the experimental period in all soils. Two compartments of adsorbed glyphosate in soil were identified as labile glyphosate and non-labile glyphosate and these reflected the strength of adsorption of the chemical. The amount of glyphosate in the labile firm for the soils ranged from 24 - 34.5% of the total and half-life ranged from 6 - 9 days. The amount of glyphosate in the non-labile form for soils ranged from 67 . 75% of the total and half-life ranged from 222 to 835 days. At 10C, the two compartments of glyphosate adsorption were identified for the Walpeup and Rutherglen soils but only one compartment could be identified in the Wimmera and Culgoa soils. Methodology was developed to permit extraction and analysis of glyphosate and AMPA in soil. Recovery of glyphosate from soils where time between fortification and extraction was only 30 sec. was 84.6 - 104%. However where extraction was delayed 13 hours, recoveries were 47.6 - 66.8%. The extractant (0.1 M triethylamine) was shown to be unable to desorb adsorbed glyphosate. Studies revealed that at 25C, the pool of extractable glyphosate was rapidly depleted by decomposition. At this temperature, the pool of extractable glyphosate was supplemented by slow desorption of non-labile glyphosate for each soil. At 10C, depletion of the pool of extractable glyphosate was considerably slower. For the Walpeup and Rutherglen soils, the rate of desorption of glyphosate from the non-labile pool was less than the rate of loss by decomposition of the herbicide. Rate of desorption of non-labile glyphosate in the Wimmera soil was shown to be the same as the rate of loss by decomposition of the herbicide. Loss of extractable glyphosate in the Culgoa soil occurred by decomposition and by slow adsorption of extractable herbicide from the labile to the non-labile form. The effects of residues of glyphosate in the field following an autumn and a summer application were investigated at selected field sites. Following the autumn application, phytotoxic activity of glyphosate was observed in sub-clover plants growing at the Walpeup and Culgoa sites but not at the Wimmera site. Growth and nodulation of plants sown up to 4 weeks after herbicide treatment were reduced at the Walpeup site. Only nodulation of plants sown up to 4 weeks after treatment was reduced at the Culgoa site. Results suggest that residues of glyphosate are only likely to significantly affect the growth of susceptible plants during winter on sandy soils. Following summer application of glyphosate, no phytotoxic activity of the herbicide was observed for sub-clover plants grown in the Walpeup sandy loam. Results suggest that in a hot summer, it is unlikely that residues of glyphosate in any soil would cause significant damage to plant growth.
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ItemStudies of barley yellow dwarf virus infection in ryegrass (Lolium spp.)( 1989)Measurements were made of the effect of barley yellow dwarf virus (BYDV) infection on the early growth of four commercial cultivars of ryegrass (Lolium spp.) under two different temperatures (24C and 16C). At 24C, BYDV infection was associated with reduced root dry weight (30-40%) in all cultivars; the effect of infection on shoot dry weight and leaf area was variable. At 16C, the effect of BYDV infection was variable, being associated with increases in both leaf area and root and shoot dry weight in one cultivar (Grasslands Arild) and decreases in another (Victorian). In two other cultivars, both leaf area and root and shoot dry weight were not significantly affected (p > 0.05) by infection with BYDV. There was no evidence of visible symptoms associated with BYDV infection. At 240C, reductions in root dry weight associated with BYDV infection were not reflected in reductions in the relative growth rates of the roots. Up to at least 28 days after inoculation (46-50 days after germination) reductions in root dry weight were associated with both aphid-feeding damage and virus infection. Experiments with the cultivar Victorian showed that shoot dry weight was not significantly affected (p > 0.05) by feeding with either viruliferous (BYDV) or non-viruliferous aphids (Rhopalosiphum padi L.). At 16C, changes in root and shoot dry weight were associated with changes in the respective relative growth rates. Both visual scoring and changes in plant dry weight were found to be unreliable criteria for assessing disease severity. Two assay systems, an enzyme-linked immunosorbent assay (ELISA) and a nucleic acid, or complementary DNA (cDNA) probe, were developed for the detection of BYDV in ryegrass. The conditions for detecting BYDV with the ELISA (the "double sandwich" variant) were optimized. Exposure of the virus to extracts of ryegrass leaf tissue for 30 min or more resulted in reductions in absorbance, with the amount of the reduction dependent on the antigen (virus) incubation temperature. The optimization of conditions for the immunoglobulin (IgG) used for coating microtitre plates (coat IgG), the IgG - alkaline phosphatase conjugate (conjugate IgG) and the antigen (virus), in conjunction with the use of polyethylene glycol - 6 000 in the preparation of the conjugate buffer, gave a rapid and reliable ELISA procedure for the consistent detection of BYDV at levels of 2 ng/ml. Both ELISA and cDNA probe techniques were used to study the incidence of BYDV isolates in perennial ryegrass at three sites (Balmoral, Mininera and Hamilton) in south-western Victoria. BYDV was detected in perennial ryegrass cultivars from the three sites, with different levels of incidence of BYDV isolates between cultivars. While the PAV-related isolate was prevalent at the three sites, the incidence of the MAV- and RPVrelated isolates varied with the site and the cultivar. One difficulty with the ELISA was that absorbance values did not fall in to distinct infected and uninfected groups within the cultivars, and at Mininera, the incidence of BYDV varied significantly between the various positive - negative ELISA thresholds that were used. At Balmoral, the incidence of BYDV determined with each threshold was compared with that determined by cDNA probing using a Spearman correlation test. A threshold of double the mean negative (uninfected ryegrass) control showed the highest correlation with the cDNA probe for a number of replicate plots at the site. The incidence of the PAV-related isolate was compared for the two cultivars Ellett and Victorian, and significant (p < 0.01) reductions in its incidence in cv. Ellett compared with cv. Victorian at the three sites, suggested that cv. Ellett is resistant to the PAVrelated isolate of BYDV. The comparative resistance of cv. Ellett to at least this isolate of BYDV indicated the feasibility of breeding and selecting for resistance to other isolates of BYDV in perennial ryegrass. In attempts to quantify resistance in perennial ryegrass, the ELISA was further developed to allow measurement of BYDV titre in ryegrass shoot tissue. In twenty selections of cv. Victorian, the performance, as measured by changes in both root and shoot dry weights, of individual selections to infection with BYDV, did not significantly (p < 0.05) correlate with virus titre measurements. Experiments with twenty selections of cv. Ellett indicated that its resistance to BYDV that was e' ident at the three field sites was not associated with resistance to aphid (Rhopalosiphum path) feeding, and consequently, transmission of the virus. Attempts to inoculate cv. Ellett with either the PAV- or RPVrelated isolates were of limited success, and it was speculated that its resistance to the virus may be due to reductions in the level of virus replication.
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ItemStudies on the ecology and control of some important plant parasitic nematodes in Victoria( 1986)Since 1965 I have been employed as a nematologist by the Victorian Department of Agriculture and Rural Affairs, based at the Plant Research Institute, Burnley. My major responsibilities have included: (1) From 1965-1975, funded exclusively by the Wheat Industry, to conduct research on the biology and ecology of the cereal cyst nematode (Heterodera avenae Woll.), and to advise on methods for controlling the disease it causes in wheat and other cereals. (2) From 1975, in addition to research on H. avenae, to conduct research on nematode diseases of other agricultural and horticultural crops in Victoria. My research has always been directed at achieving methods of control which are both practical, and economic to apply. Prior to 1965, cereal cyst nematode was recognised as a major cause of disease in cereals in the southern wheatbelt of Australia; its distribution was known to be related to well structured soils; knowledge of its biology and ecology under Australian conditions was limited; and, crop rotation was the only recommended method of control. There were no sources of resistance suitable for use in breeding programs; the existence of pathotypes was unknown; and, the extent and magnitude of yield losses had not been determined. My research has culminated in the adoption by cereal growers, of several new control strategies, and the results have lead to the establishment of a new pesticide market (valued at millions of dollars per year), and economic benefits from the use of nematicides are already being obtained by rural communities in Victoria and South Australia. The results of this, and other research on various aspects of the biology and control of nematodes causing disease in grapevines, citrus, pastures, vegetable crops, ornamentals, etc. are presented in the papers which follow.