School of Agriculture, Food and Ecosystem Sciences - Theses
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ItemMaking the connection between history, agricultural diversity and place: the story of Victorian apples( 2016)Apple growing practices are embedded in a productivist mentality aiming for ever higher efficiency and productivity. And while the climate change impacts are to a large extent known, there is little attention given to the coupling of the social and the ecological effects. I use apple growing as a case study to explore the relationship between place, biodiversity and rural change in Victoria. My research is based on historical research; including an analysis of the Museum Victoria’s collection of wax apple models, and in-depth interviews with orchardists. By drawing on environmental history, social-ecological systems thinking and Bourdieu's theory of practice, I highlight the importance of a systems perspective and inform it by emphasis on the critical role of underlying power structures and individual dispositions, or the habitus, of the growers. These dispositions have been shaped and internalised by the growers’ histories and their physical surroundings. Orchardists have been able to respond to intensifying production requirements by utilizing technologies and scientific nous to keep up with the continuous aim for efficiency. Growers are caught up in a self-reinforcing cycle of satisfying the demand for perfect apples by adopting expensive techno-scientific approaches to enable ever more intensive production. The symbolic violence and amplified biophysical pressure orchardists experience has driven many to despair; resulting in a significant decline in small scale apple growing businesses over the last decade. I offer some suggestions for government policy and support measures and argue that any services or support programs need to be tailored to the appropriate level and need of each orchard business and the individuals who are involved. My analysis shows that those growers, who engage more closely with their biophysical place as well as their history and identity as apple growers in that place are (re-)creating another version of what it means to be an apple grower. In some cases this is resulting in resistance to the vortex of agricultural productivism that has been the basis of their existence for many generations.
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ItemStudies of Ascochyta rabiei in Australia( 2005)Ascochyta rabiei (teleomorph: Didymella rabiei) which causes ascochyta blight is the most serious disease of chickpea (Cicer arietinum) in Australia as it causes significant losses in crop yield and quality. Although A. rabiei is heterothallic and genetically diverse elsewhere in the world, a study carried out on Australian isolates collected between 1995 and 2000 identified only one mating type and a low level of genetic diversity within the Australian A. rabiei population. In 2002, ascospores of Didymella rabiei, the sexual state of A. rabiei, were trapped in a discharge chamber, from chickpea stubble naturally infected with ascochyta blight in Western Australia. Examination of the stubble revealed pseudothecia typical of Didymella rabiei. The reported presence of the teleomorph in Western Australia indicated that the second mating type had been introduced into Australia or that the pathogen was capable of a low level of homothallic compatibility, previously undetected. The aims of this research were, to undertake a new survey of Australian A. rabiei isolates, to test for the presence of the second mating type, to determine if there has been a change in the diversity of the Australian population and to investigate if pathogenic variability was displayed among isolates. Sixty-seven isolates collected from chickpea fields in South Australia, New South Wales, Queensland, Victoria and Western Australia during the 2003 cropping season were single spored and confirmed as A. rabiei using a PCR test. The isolates were typed for mating type using MAT gene specific PCR primers and compared with tester isolates from USA. This test revealed that all the 67 isolates belonged to mating type 2 (MAT 1-2), thus, the presence of mating type 1 (MAT 1-1) in Australia could not be confirmed. Sequence Tagged Micro Satellite (STMS) markers were used to examine the genetic diversity of the A. rabiei isolates. The isolates were assessed for allelic variation at 19 microsatellite loci, each of which amplified a single locus. Seven of the loci were polymorphic across all the 67 isolates, while the remaining twelve were monomorphic. Based on the allele profiles at the seven polymorphic loci, 19 distinct A. rabiei haplotypes were identified with a total of 33 alleles. One haplotype constituted 35.8 % of the population and was found in the collections from South Australia, New South Wales, Queensland and Victoria. Cluster analysis did not show a clear distinction between isolates based on the state from which they were collected. Polymorphism across the 19 microsatellite loci revealed a slight elevation in diversity in the 2003-2004 population (Ht = 0.07; compared to 0.02 in the 1995 to 2000 collection) and an increase in the number of haplotypes compared with that detected in the previous study of Australian isolates. To examine the pathogenic variability of the Australian population of A. rabiei, nine isolates were inoculated on five chickpea differentials, ranging from highly susceptible to resistant, under controlled conditions optimal for A. rabiei growth and infection. Eight of the isolates were virulent on the susceptible and intermediate chickpea cultivars but not the resistant cultivar and one isolate was only virulent on the susceptible cultivar. Based on these results the isolates were classified into two pathotype groups. The results obtained from the study of the population structure and the pathogenic variability of A. rabiei in Australia will enable the Australian chickpea breeders to understand the A. rabiei population better for formulating management and breeding strategies.
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ItemEffects of adding nutrients on soil chemistry and tree growth in native Eucalyptus forests of south-eastern Australia( 2007)The decreasing area available for timber extraction in south-eastern Australia, due largely to social pressure to reserve greater areas of forest, has led to the consideration of fertiliser-application to increase wood output from the remaining available forest. Potentially deleterious effects of fertilising on water quality must be assessed before implementation on a wide scale. This is in accordance with relevant forest management policies. This study examined the effects of applying fertilisers containing nitrogen and phosphorus, on soil and soil-water chemistry in two pole-sized stands of mixed Eucalyptus spp in the Wombat Forest, in the Midlands Forest Management Area, Victoria, Australia. The findings are synthesised and discussed in relation to management of regenerating mixed-eucalypt forests in south-eastern Australia. Fertiliser treatments were none (R); 400 kg N ha-1 as ammonium-sulphate (N); or 400 kg ha-1 plus 202 kg P ha-1 as triple superphosphate coated with 10% sulphur (NP). It was calculated that incidental additions of S were 1371 kg ha -1 (N treatments), and 1696 kg ha-1 (NP treatments). It was expected that P would be principally adsorbed on soil surfaces; N immobilised in the soil organic pool and that metallic cations would enter the soil solution to varying degrees. Fertiliser-addition increased both plot-basal-area (BA) growth and the rate of stand self-thinning. In 3.8 years, BA in reference (R) plots at two sites increased by 7.3% and 23.4%. Where N alone was added, BA increased by 14.2% and 27.1%, while in NP plots BA increased by 17.1% and 42.7% respectively. Mortality was 9% in untreated plots compared to 14% in NP plots. Estimated increases in biomass growth equated to additional above-ground nutrient accumulation of 0.4 to 1.5 kg ha-1 of P, and 5.5 to 20.8 kg ha-1 of N. This represented only 0.2 to 0.7% of added P, and 1.4 to 5.2% of added N. Soil solution was extracted from 10 and 50 cm with porous-ceramic-cup tension-lysimeters (-0.6 kPa). Concentrations of P and N were low both before and after adding fertiliser. Across all treatments the maximum median PO43- concentration in soil-water at 50 cm was 0.12 ppm (mean 0.28 ppm). Typically PO43- concentrations were not higher than 0.03 ppm. The 400 kg ha-1 of added N was rapidly immobilised in the soil organic pool. The greatest mean NH4' concentration from a single sampling occasion was 1.1 ppm. The mean NO3 concentration at 50 cm was never higher than 0.26 ppm. After adding N in fertiliser the proportion of NO3- relative to NH4* in soil-water increased and was correlated with decreasing soil-water pH. Less than 1% of added P and N was recovered from soil solution at 50 cm. The largest pool of added P recovered was PO43- adsorbed to soil between 0 and 20 cm, due to the soil adsorption capacity being well in excess of the applied 202 kg P ha-1. Phosphate desorption using sequential extractions with a mild acid extractant (0.3M NH4F, 0.1M HCI) recovered between 25% and 116% of added P. Differences were attributed to both the amount of P added and the effect of time since treatment at different sites. Soil disturbance during sampler installation was found to be more likely to raise soil-water P concentrations at 50 cm than would adding up to 202 kg P ha-1. Among the ions in solution. SO42- and CI' were the dominant anions while Cat+ dominated the cation chemistry. In untreated forest 5042- in soil-water ranged from 7.7 to 16.0 ppm at 10 cm and 7.9 to 12.2 ppm at 50 cm. In fertilised plots up to 100.5 ppm SO42 was measured in soil-water at 50 cm depth. In the N treatment at 50 cm, SO42- in soil-water accounted for 9.4 % of applied S. compared to 14.0 % in NP. In untreated forest, soil-water Cl- and SO42- accounted for over 98% of the total soil-water anions, in roughly equal proportions at 10 cm, and CI- slightly higher at 50 cm. Following fertiliser-application soil-water pH at 10 cm fell from 6.3 in R to as low as 4.81 (N) and 4.45 (NP). At 50 cm pH never dropped below 6 and there were no visible departures from reference concentrations. Relative activities of K+ and Mg2+ in solution increased with decreasing pH, indicating increased leaching potential. Sulphate in soil-water increased total anion charge further in NP than in N. Total charge (cmolc L-1) for cations followed anions. A slight deficit in anion charge was likely due to the unquantified contribution of organic anions. These results confirm that despite the quantity of fertilisers added in this trial being double likely operational quantities, the forest and associated soils had the capacity to retain these nutrients through a variety of processes. The study validates the environmental sustainability of proposed intensive management practices including fertiliser-application in this forest type. It also emphasises the importance of understanding fundamental forest nutrient cycling processes when aiming to carry out intensive forest management practices in an environmentally sensitive manner.
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ItemEffects of organic applicants in a southern Victorian vineyard( 2007)Mulch is a material applied to the surface of the soil to reduce weed growth and reduce soil moisture loss through evaporation from the soil surface. The use of organic mulches will alter the soil environment. This alteration may include reducing soil temperature fluctuations, increasing soil organic matter, increasing soil microflora populations modifying soil chemical properties and increasing soil moisture retention. An experiment was conducted to compare composted green waste mulch and barley straw mulch with herbicide as alternative means of maintaining the undervine strip in a cool climate vineyard. Plant and soil responses to the different undervine treatments were monitored. Grapevine budburst was retarded, however, by the fourth week of vine growth there were no observable differences in grapevine growth stage. Both mulches stimulated grapevine growth and increased yield, with the compost mulch increasing vegetative growth with respect to fruit yield. The fruit quality parameters juice pH and titratable acidity were not significantly altered by the different undervine treatments. In the second year of the experiment the juice soluble solids were lower on the straw mulched grapevines. The compost mulch increased soil pH and carbon levels. The straw mulch improved soil water retention and the mass of soil fungal hyphae. Both mulches increased soil cation exchange capacity. The straw mulch increased soil exchangeable Mg to a greater extent than was predicted from straw nutrient content. The significant variations seen in soil cation content under the mulch treatments were not observed in the tissue analysis. Mulch can be used in a cool climate vineyard to increased yield without deleterious side effects.
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ItemPhotosynthetic responses to light, nitrogen, phosphorus and pruning of Eucalyptus in south-eastern Australia( 2005)Eucalypts frequently grow faster after additions of fertiliser, but more slowly in the shade or following `green pruning'. The coupling of rates of growth to environmental factors is at least partly due to acclimation of photosynthetic processes. Photosynthesis rarely proceeds at maximum rates in natural environments as photosynthetic processes and the supply of basic requirements of photosynthesis (CO2, H20, light, phosphorus and nitrogen) vary at both short (minutes to hours) and longer (days to months) time scales. Currently we lack mechanistic explanations for how these variables, alone and in combination underpin changed growth rates in Eucalyptus. This study examined growth and photosynthetic characteristics in glasshouse-grown seedlings and field-grown trees of Eucalyptus species that are commonly planted for forestry and revegetation purposes in central Victoria. Acclimation to light (among seedlings and within canopies), nutrient availability (phosphorus and nitrogen) and increased sink-strength for photosynthates were the primary foci of the study. In each instance I examined distribution of leaf nutrients within a canopy and allocation of N to Rubisco and chlorophyll to assess the degree to which nutrients limit photosynthesis in Eucalyptus. A novel technique was introduced to quantify the allocation of inorganic phosphorus within cells (cytoplasm versus vacuole), followed by an assessment of inorganic phosphorus allocation in response to a long-term reduction in phosphorus supply. In all circumstances, rates of growth were responsive to environmental conditions. Growth responses were underpinned by altered patterns of biomass partitioning and changed leaf morphology more than by rates of photosynthesis per se. There was little difference in adaptive strategies implemented by seedlings and trees: both were oriented towards the accumulation of nutrients rather than increasing rates of photosynthesis. Photosynthesis was reduced by shading (among different plants and within the canopy of a tree) and reduced phosphorus supply whereas N had little effect on photosynthesis. Analysis of pools of inorganic P revealed that adequate supplies were maintained for photosynthetic processes regardless of P supply, therefore reduced photosynthesis follows, rather than leads, a more general leaf-level response to reduced P. Similarly, changed partitioning of nitrogen between Rubisco and chlorophyll was unnecessary as leaf nitrogen concentrations were consistently maintained at well above published minimum levels. Hence, photosynthesis was not up-regulated following increased nitrogen or phosphorus supply; instead excess nutrients were accumulated and used to support increased biomass. One exception was after defoliation, when up-regulation of photosynthesis was observed, presumably to ensure the demand for photosynthates could be met by a reduced leaf area. Sensitivity analyses consistently revealed variation in photosynthetic rates owed more to altered biochemical activity (e.g. Jmax and Vcmax) rather than stomatal conductance regardless of growth condition (glasshouse versus field). Hence, whilst Eucalyptus has considerable photosynthetic potential, faster rates of carbon fixation are only exhibited in the short-term. In part, this is due to the multiplicity of factors involved in `optimisation' of photosynthesis and their individual and collective responses to environmental conditions. In the long term however, increased canopy photosynthetic capacity follows only an increased photosynthetic area.
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ItemThe effect on some milking characteristics of cows of changing the flow rate thresholds of automatic cluster removers in a seasonally calving dairy herd( 2004)An experiment to investigate the effects of altering the end-of-milking point values of automatic cluster removers (ACRs) from 0.40 kg/min (Control) to 0.70 kg/min (Fixed ACR), or 1.00 kg/min (Rising ACR) over an extended period from the peak until the end of lactation of a seasonally calving pasture based herd has been conducted at the Victorian Department of Primary Industries Ellinbank research dairy. One hundred and ninety two cows. and heifers were used in the experiment (64 animals statistically allocated to each treatment group). A comparative analysis of results for each treatment group is presented for average milk yield, average milking duration, average maximum milking duration, average milk flow rate, average peak milk flow rate and udder health. The Fixed ACR treatment reduced the average daily milking duration by up to 7.6%, but only for a small portion of the treatment period. The Rising ACR treatment reduced the average daily milking duration significantly by up to 15.3%, for the majority of the treatment period. The Fixed ACR treatment did not reduce the average daily maximum milking duration whilst the Rising ACR treatment reduced the average daily maximum milking duration significantly by up to 17.9%, for the more than half of the treatment period. A marginal increase in average milk flow rate was observed for the Rising ACR treatment. Even though the Rising ACR treatment began the experiment with higher average peak flow rate for AM and PM it declined less than the Fixed. ACR or Control treatment throughout lactation, providing a mechanism for the, removal of the same amount of milk in a reduced time when compared with the other treatments. However the difference observed in peak flow rates was not statistically significant. No difference was found amongst the treatment groups when analysed for new cases of sub-clinical or clinical mastitis. The geometric mean of individual cow cell counts was significantly reduced for the Rising ACR treatment in the latter stages of lactation. Flow rate curves have been constructed from real milking data and are presented as examples of the large variation of actual- flow rate from a cows udder. The flow rates curves are used to track individual cows through lactation to observe changes in the cow's milking characteristics. Theoretical consideration is given to how particular types, or brands, of ACR operate under various conditions and it is shown that the results from this experiment could be replicated by using other types of ACR if they are managed correctly. This study has shown that significant labour productivity. benefits could be achieved by, increasing the ACR end-of-milking point settings to 1.00 kg/min without impacting milk yield. However, further work is suggested to ensure that udder health is not compromised. This is to certify that the thesis comprises only my original work except here indicated in the preface; due acknowledgement has been made in the text to all other material used; the thesis is 19400 words in length, inclusive of footnotes, but exclusive of tables, maps, appendices and bibliography.