School of Agriculture, Food and Ecosystem Sciences - Theses

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    The role of tubulins in secondary cell wall deposition in woody tree species
    Machado Tobias, Larissa ( 2020)
    Woody trees are an essential source of timber, pulp, paper and biofuel, and advances in biotechnology provide opportunities for the improvement of traits of interest for specific end uses. Cellulose microfibrils, the basic structural component of plant cell walls, are responsible to a large degree for wood mechanical and physiological properties. The angle between the direction of the helical windings of cellulose microfibrils in plant secondary cell walls, or microfibril angle (MFA), plays critical roles in a tree’s development and has become a subject of major interest in forest biotechnology, particularly in detailed studies of the secondary cell wall of xylary (wood) cells. While our knowledge of how exactly the cellulose synthase complex (CSC) acts in response to environmental and genetic cues remains sketchy, guidance of cellulose deposition has been repeatedly accredited to microtubules, a cytoskeleton component formed of protein dimers of alfa- and beta-tubulin. Nevertheless, few studies explore the cytoskeleton roles in secondary cell wall deposition in woody tree species. Reaction wood (RW) develops in response to gravitational stimulus through a series of changes at the cellular and molecular levels. Tubulin genes have been previously reported to be upregulated during RW formation and differences in their expression might lead to differences in microtubule assembly. This differential microtubule organisation might be related to changes on cell wall morphology, including MFA. In this study, cortical microtubule array organisation was therefore assessed in samples from trees forming RW and stems growing upright (normal wood). To further investigate if perturbation of microtubule organisation would impact wood formation, microtubule-interacting drugs were applied to wood tissue depositing SCW in vivo and in vitro. Together, results indicate that tubulins play an essential role in cellulose deposition in the secondary cell wall of woody tree species to ensure appropriate microfibril orientation.