School of Agriculture, Food and Ecosystem Sciences - Theses
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ItemStudies of Ascochyta rabiei in Australia( 2005)Ascochyta rabiei (teleomorph: Didymella rabiei) which causes ascochyta blight is the most serious disease of chickpea (Cicer arietinum) in Australia as it causes significant losses in crop yield and quality. Although A. rabiei is heterothallic and genetically diverse elsewhere in the world, a study carried out on Australian isolates collected between 1995 and 2000 identified only one mating type and a low level of genetic diversity within the Australian A. rabiei population. In 2002, ascospores of Didymella rabiei, the sexual state of A. rabiei, were trapped in a discharge chamber, from chickpea stubble naturally infected with ascochyta blight in Western Australia. Examination of the stubble revealed pseudothecia typical of Didymella rabiei. The reported presence of the teleomorph in Western Australia indicated that the second mating type had been introduced into Australia or that the pathogen was capable of a low level of homothallic compatibility, previously undetected. The aims of this research were, to undertake a new survey of Australian A. rabiei isolates, to test for the presence of the second mating type, to determine if there has been a change in the diversity of the Australian population and to investigate if pathogenic variability was displayed among isolates. Sixty-seven isolates collected from chickpea fields in South Australia, New South Wales, Queensland, Victoria and Western Australia during the 2003 cropping season were single spored and confirmed as A. rabiei using a PCR test. The isolates were typed for mating type using MAT gene specific PCR primers and compared with tester isolates from USA. This test revealed that all the 67 isolates belonged to mating type 2 (MAT 1-2), thus, the presence of mating type 1 (MAT 1-1) in Australia could not be confirmed. Sequence Tagged Micro Satellite (STMS) markers were used to examine the genetic diversity of the A. rabiei isolates. The isolates were assessed for allelic variation at 19 microsatellite loci, each of which amplified a single locus. Seven of the loci were polymorphic across all the 67 isolates, while the remaining twelve were monomorphic. Based on the allele profiles at the seven polymorphic loci, 19 distinct A. rabiei haplotypes were identified with a total of 33 alleles. One haplotype constituted 35.8 % of the population and was found in the collections from South Australia, New South Wales, Queensland and Victoria. Cluster analysis did not show a clear distinction between isolates based on the state from which they were collected. Polymorphism across the 19 microsatellite loci revealed a slight elevation in diversity in the 2003-2004 population (Ht = 0.07; compared to 0.02 in the 1995 to 2000 collection) and an increase in the number of haplotypes compared with that detected in the previous study of Australian isolates. To examine the pathogenic variability of the Australian population of A. rabiei, nine isolates were inoculated on five chickpea differentials, ranging from highly susceptible to resistant, under controlled conditions optimal for A. rabiei growth and infection. Eight of the isolates were virulent on the susceptible and intermediate chickpea cultivars but not the resistant cultivar and one isolate was only virulent on the susceptible cultivar. Based on these results the isolates were classified into two pathotype groups. The results obtained from the study of the population structure and the pathogenic variability of A. rabiei in Australia will enable the Australian chickpea breeders to understand the A. rabiei population better for formulating management and breeding strategies.
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ItemA new virus disease of carrots : its transmission, host range, and control( 1948)During 1940 commercial vegetable growers in the Melbourne market garden area requested an investigation of a disease of spring sown carrots, which had been occurring over a number of years. Summer sowings were not affected, but the failure, or partial failure, of spring sowings had made the production of this crop uneconomic, and as a result, the continuity of carrot supplies to the Melbourne market had been disrupted. The principal objects of the investigation described in this paper have been the elucidation of the factors responsible for the disease, and the development of practical disease control measures. A preliminary report of the investigation, which commenced in 1941, has been published elsewhere (Stubbs & Grieve, 1944).
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ItemEpidemiological and physiological studies of the effects of peach rosette and decline disease on the peach, prunus persicae L. Batsch( 1975)The incidence in the field of the disease peach rosette and decline (PRD), which is of considerable economic importance in the Goulburn Valley, causing fruit loss and tree death, was shown to increase from 0.9 to 91.3% in an orchard of cv. Golden Queen in 10 years. Similar results were found with the cv. Pullars Cling, in which infection increased from 1.5 to 29.7% over five years. The pattern of spread was mainly from infected trees to contiguous uninfected trees. This is consistent with the view that the main causal agent, prune dwarf virus (PDV), is transmitted only via the transfer of infected pollen : a previous finding in cherries which was confirmed in peaches. Prunus necrotic ringspot virus (PNRV) is the other virus always present in the field in PRD-infected trees. The mode of spread of PNRV is also by pollen. Within the tree, PDV moved erratically from the first infected limb, via phloem but not xylem, into the other limbs well in advance of the appearance of symptoms. Three months after flowering, PDV was detected in 65% of main limbs adjacent to the first infected limbs but in only 30% of limbs more remotely positioned on the tree. However, removing infected limbs within four weeks of flowering, when the initial infection was presumed to have occurred, did not prevent the movement of PDV into the rest of the tree. Laterals from peach trees infected with PRD were tested for the presence of PDV, using woody virus indicators (cvs Golden Queen, Italian Prune and Elberta seedling). Golden Queen was found to be a more reliable indicator for detecting PDV than Italian prune, as the presence of PNRV with PDV killed 71% of the Italian prune buds compared to only 34% of the Golden Queen buds. Golden Queen also developed more obvious foliage symptoms of PDV infection than Elberta seedlings. The probability of failing to detect PDV in infected field trees, using all three indicator plants, was higher in the first year of infection. The rate of spread of PRD was reduced in the orchard by preventing infected trees from flowering, either by removing obviously infected trees or by deblossoming. Removing infected trees resulted in a three-fold reduction in the spread of the disease in two seasons. Removing the flowers from infected trees before pollination reduced the spread of the disease by about half. This, only partial, control of the spread of PRD by tree removal or deblossoming was attributed to the presence of up to 14.3% of trees without symptoms being latently infected with PDV. It was observed that deliberate infection with PDV by pollen also resulted in a slow expression of the symptoms of PRD. The effects of PRD on the growth of young peach trees was obvious in the first three months of growth. There were considerable varietal differences in the severity of this effect. Those varieties based on cvs. Golden Queen or Levis Cling were more severely affected than the variety Elberta. The results from shoot elongation measurements agreed with those obtained from conventional growth analysis methods. These latter experiments showed that, after three months, the dry weight and leaf area of infected Golden Queen plants were reduced by 94%. The fruit yield from mature PRD-free trees was three times that of trees infected for the first season, even though symptoms were apparent only on one limb; and six times that from chronically affected trees infected for two seasons. The effect of virus infection on the photosynthetic ability of single, attached peach leaves was studied under laboratory conditions using infra red gas analysis. The constants derived from the equations describing the relationship between net photosynthesis (Pn) and both irradiance and CO2 concentration were used to analyse the effects of infection by PRD on photosynthetic characteristics of the leaf. The asymptotic value of Pn (Pmax) in young leaves was reduced 15% by PRD-infection, mainly through an increase in the "residual resistance" to 002 diffusion and a decrease of 23% in the parameter indicating photochemical efficiency. There was also evidence that the gas phase resistance was higher in infected leaves at low levels of irradiance. Dark respiration was 51% higher in infected leaves, but this difference was not significant. PRD did not reduce Pn in 60-day-old leaves, normal leaf senescence having a predominant and greater effect. It was concluded that PRD infection had its large effects on growth via a reduction in leaf area; the effects on the photosynthetic capacity per unit leaf area being minor. An effect of PRD infection on the translocation of 14C-assimilatesout of leaves was also observed. Infected leaves retained twice the assimilates than did uninfected leaves. It is concluded that the most promising methods of control of PRD include removal of infected trees, deblossoming suspected infected trees until diagnosis is confirmed, use of virus-tested plants, the gradual destruction of infected orchards and protecting young, healthy orchards from infection either by barrier crops or deblossoming the young plants until they reach an economic bearing age.
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ItemStudies on the etiology and control of parsnip canker, caused by itersonilia pastinacae channon( 1968)In Victoria, parsnip canker is caused chiefly by Itersonilia pastinacae Channon and, less frequently by Phoma sp.. Inoculation of both fungi into parsnip roots produced typical symptoms of the disease. The morphology of the local isolates of I. pastinacae was compared with others described and the taxonomy of the genus discussed. The fungus was shown to be seed-borne, the infection being carried in plant debris contaminating the seed and also externally on the seed itself. An aerated-steam heat treatment was developed to eliminate this seed-borne infection. In field studies I. pastinacae in naturally infected roots survived at least 12 months burial in soil. When the root was mutilated by removing the stem apical meristem, to promote rapid decomposition, the fungus survival period was reduced to 6 months. In laboratory studies the fungus, impregnated in fibre glass mesh, survived 6 months burial in unsterile soil, whereas the survival period in sterile soil was at least 12 months. Survival was shown to be due to the presence of chlamydospores; mycelium and ballistospores being rapidly lysed. Chlamydospores germinated directly to form ballistospores. I. pastinacae, also had a low competitive saprophytic ability. Field and laboratory studies demonstrated that ballistospore discharge was influenced chiefly by high relative humidity, the presence of rain being more effective than dew. Spore discharge followed a diurnal periodicity, the maximum discharge occurring between 08.00 and 10.00 hr.. Low temperature, however, reduced spore discharge even at optimum R.H. Ballistospore germination studies demonstrated soil fungistasis an effect destroyed by sterilization of the soil. Germination of ballistospores was also influenced by nutrients, either diffusates from parsnip roots and leaves or by a 2% glucose solution. Strepstomyces sp. and Bacillus subtilis were isolated from soil and antagonism towards I. pastinacae exhibited. Cell-free culture filtrates of both antagonists lysed I. pastinacae while incorporation of the filtrates into media reduced the growth rate of the fungus. Sterile soil inoculated with the antagonists also lysed I. pastinacae. Foliage applications of fungicides, particularly those containing copper, reduced the incidence of parsnip canker in the field. Control was also achieved in the field by keeping the developing crowns covered with soil during the growth of the crop.
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ItemA study of pathogens associated with stunted patches and deadheads in Victorian wheat crops( 1968)Between 1959-1966 seven species of root-rotting fungi and barley yellow dwarf virus were consistently associated with stunted patches and "deadheads" in Victorian wheat crops. The fungi occurring in order of frequency were: Fusarium culmorum (W.G.Sm) Sacc., Curvularia ramosa (Bainier) Boedijn, Helminthoaporium sativum Pammell, King and Bakke, Pythium ultimum Trow var. ultimum, Rhizoctonia solani Kuhn, Ophiobolus graminis Sacc. and Fusarium graminearum Schwabe. Barley yellow dwarf virus was found each season in the higher rainfall areas of the State; in 1961 and 1965 it occurred in epidemic proportions in all districts. These organisms may be divided into three groups based upon their ability to reduce yield of wheat under controlled glasshouse experiments. (1) MAJOR REDUCTION BY: Barley yellow dwarf virus; Ophiobolus graminis; Helminthosporium sativum. (2) MINOR R RUCTION BY: Fusarium graminearum, Fusarium culmorum; Rhizoctonia solani; Pythium ultimum. (3) NO REDUCTION BY: Curvularia ramosa Ophiobolus graminie and barley yellow dwarf virus are the most important of these pathogens in Victoria. Both caused yield losses of approximately 45-60% in the field. Under controlled glasshouse conditions they caused highly significant yield losses. Five other fungi investigated caused some losses, but during the period 1959-66 were not of great economic importance. The incidence of these pathogens could be related to climatic conditions before and during the wheat growing season. (1) Highest incidence after a dry winter and wet spring: Ophiobolus graminis; Pythium ultimum. (2) Highest incidence after a mild winter and dry spring: Barley yellow dwarf virus; Fusarium graminearum; Fusarium culmorum; Helminthosporlua ; Curvularia ramosa. (3) Highest incidence when crop sown under wet conditions: Rhizoctonia solani; Pahiuta ultimum.
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ItemA study of the fungi associated with the decline of subterranean clover pastures in Victoria( 1972)A general study of the problem of decline of subterranean clover (Trifolium subterraneum L.) in Victorian pastures indicated that Fusarium spp., especially F. oxysporum are the most common fungi associated with rotted roots of affected plants. Moisture stress due to waterlogging was shown to be conducive to the fungal root attack of subterranean clover in pot studies, indicating that this is an important factor causing clover decline in the field. Evidence was obtained supporting the recognition of Fusarium avenaceum (Fr.) Sacc. as an important seedling pathogen of subterranean clover. Studies of the pathogenicity of this species at a range of temperatures were conducted in controlled environment chambers. The results suggested that increasing temperature has a negligible effect on the incidence of infection, but increases the severity of disease after infection.
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