School of Agriculture, Food and Ecosystem Sciences - Theses
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ItemNutritional modification of muscle long-chain omega-3 fatty acids in lambs : effects on growth, and composition and quality of meat( 1999)Three experiments were conducted to investigate the effects of dietary supplements rich in omega-3 fatty acid on muscle omega-3 fatty acid deposition. The consequential effects on growth performance of lambs, colour and lipid oxidative stability of muscle over refrigerated display, and the sensory properties of cooked meat were also examined. A mixture of lucerne chaff : oaten chaff was used as basal diet, offered in different proportions were fed to lambs ad libitum (Expt. 1) or at 90% ad libitum (Expts. 2 and 3). Such mixtures of roughage diet support slow growth and provide a feed quality pattern similar to late spring to late summer pasture. In Expt. 1, fish meal (7%), canola meal (8%) and soymeal (7%) as natural feed supplements were compared in lambs fed low quality roughage diet. In Expt. 2, fish meal (9%) and oilseed supplements either in unprotected form (rapeseed - 7%) or in protected form (ground canola seed - 6%) were examined in lambs on medium quality roughage diet. Lipids and the proteins in the ground canola seed were treated (RUMENTEK) with aldehyde to protect them from the rumen microbial activity. Fish meal (9%), fish oil (1.5%), fish oil (1.5%) with sunflower meal protein (9%),' and sunflower meal protein alone (10.5%) (a commercial product of a protein supplement from RUMENTEK) were compared in lambs fed medium quality roughage diet in Expt. 3. Long-chain omega-3 fatty acids (eicosapentaenoic acid + docosahexaenoic acid) in muscle longissimus thoracis was increased modestly and markedly with fish meal and fish oil alone or with sunflower meal protein diet, respectively. These long-chain fatty acids were deposited in the muscle structural phospholipid rather than in storage triglycerides. All the diets mentioned above also significantly reduced omega-6:omega-3 fatty acid ratio in meat which is another beneficial effect, as the dietary recommendation in many countries has been to reduce the ratio of omega-6:omega-3 in human diet. Soymeal diet increased modestly both the omega-3 and omega-6 fatty acid content of muscle longissimus thoracis resulting in no differences in the omega-6:omega-3 ratio of the meat. A supplement of protected canola seed significantly increased the precursors of omega-6 (linoleic) and omega-3 (linolenic) but not the long-chain analogues such as arachidonic acid (omega-6) and eicosapentaenoic, docosahexaenoic acid (omega-3), respectively. The marked increase in linoleic acid content was in both triglyceride and phospholipid fractions of muscle longissimus thoracis but the modest increase in linolenic acid content was only in triglyceride fraction of meat. Supplements of canola meal used in Expt. 1, unprotected rapeseed used in Expt. 2 and protected sunflower meal protein used in Expt. 3 did not alter the fatty acid composition of muscle longissimus thoracis compared with lambs fed the control diet in that particular experiment. The increased level of long-chain omega-3 fatty acid and/or omega-6 fatty acid with the lipid supplements discussed above did not significantly affect the meat colour stability and lipid oxidative stability of fresh and vacuum packaged meat over the storage at refrigerated display. This suggests that the conditions under which the animals are grown (grazing vs grain fed or feedlot) and the species of animal are important in determining the oxidative stabilities of meat by altering the levels of muscle vitamin E concentrations at slaughter. The level of inclusion of lucerne chaff in the basal diet is an important factor in improving the redness of meat indicated by the a*-value; a higher level of lucerne chaff intake is more likely to be associated with increased intake of vitamin E. Thus colour and lipid oxidative stabilities of meat can be improved in red meat animals that are on poor quality diets by the inclusion of lucerne chaff in their diet. The sensory properties of cooked meat evaluated in the present study were not affected by the significant increase in muscle long-chain omega-3 fatty acid or omega-6 fatty acid content with fish oil and protected canola seed supplements, respectively. Addition of protected sunflower meal as a protein supplement together with fish oil significantly lowered the ratings of flavour and overall acceptability of meat compared with the control lambs. The results demonstrate that the common `lamby' and `muttony' flavour and aroma attributes were not hidden by any of the dietary treatments. These two characters associated with the species flavour and aroma were recognised by the panellists as a distinct attribute. Dry matter intake was not adversely affected by any of the lipid supplements used in the present study. Feed conversion efficiency was highest with fish meal diet on both low and medium quality roughage diets. At medium quality roughage-based diet, Feed conversion efficiency was modestly improved by protected canola seed diet but other supplements providing either natural (unprotected rapeseed) or protected protein (protected sunflower meal) did not support significant differences compared with basal diet. The significant increase in liveweight gain with fish meal diet reflected a significant increase in hot carcass weight compared with all other supplemented lambs either on low or on medium quality roughage diet. Protected lipid and protein offered by protected canola seed diet significantly and moderately increased liveweight gain and hot carcass weight from control diet but not different from unprotected rapeseed diet. The greatest muscle deposition was with the fish meal diet and is attributed mainly to the increased amount of protein and energy absorbed from the small intestine of those lambs. In addition to energy and protein absorption, the alteration of omega-3 polyunsaturated fatty acids in muscle membranes may have a further influence in lean meat production. In terms of carcass gain and intramuscular fat deposition of fishmeal and fish oil fed lambs, the results also lead to a hypothesis that modifying omega-3 polyunsaturated fatty acid of muscle membrane phospholipids may have an influence in improved muscle deposition in lambs by improving the insulin action at skeletal muscle site.
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ItemTree growth modelling of Eucalyptus delegatensis (R. T. Bak.) and other eucalyptus species utilising early-aged stand measurements( 1999)Growth modelling methods for Eucalyptus species that can make use of minimal measurement information are scarce but necessary for those involved in small-scale forestry to make informed decisions about investment and management options. A computer program, FARMTREE, is available for evaluating the costs and benefits of trees on farms, however its growth modelling functions could be improved. This thesis achieves this for Eucalyptus delegatensis (R. T. Bak.) and provides a series of stand basal area, survival, diameter distribution and individual tree diameter increment models that could be incorporated into a FARMTREE-like program. While not a major farm forestry species, the models have been related to other Eucalyptus species and when more later-age data become available, the methods used here could be fully applied to more suitable species. A systematic series of growth predictions were made, with estimates from the earlier processes being used in the next. Initially, the Gompertz function was selected as the best of five non-linear equations and used to model stand basal area based on two, early-age measurements, while keeping the asymptotic parameter constant. Survival was modelled using a modified logistic function with basal area and age as the predictor variables. The results from the above procedures were then used to model diameter distributions using a percentile-based parameter recovery procedure. The predicted distributions were assessed by comparing them to the actual cumulative density functions. Comparing the predicted estimates of basal area, mortality and diameter distributions to those produced by FARMTREE showed that the new estimates were better, both for the stands for which the models were developed, and for an independent data set. Individual tree diameter increment models based on distance-independent competition indices were also studied. Overall, the predictions for two-year diameter increment were not as good as for the previously studied areas of growth. This was especially noticeable when observing the results for the verification data set. These poorer results for diameter increment may be because of the less flexible methods used or perhaps partially due to the accumulation of previous prediction errors. The research approaches and functions used are easy to apply, efficient, accurate and require minimal actual growth measurement information. They could be applied to other species in greater detail when more information becomes available and could be incorporated into FARMTREE or a similar package for use by owners and managers of small farm forests with the expectation of providing improved growth predictions.
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ItemThe effect of growing location and time of sowing on the production of premium quality oilseeds in south-eastern Australia( 1998)New Brassica oilseeds with modified fatty acid profiles tailored to specific end-uses are being developed in southern Australia. However, the fatty acid composition of Brassicas are known to vary markedly with environmental conditions during growth. A series of experiments were conducted to establish the most appropriate regions and sowing times within south-eastern Australia for the production of high oleic acid canola (HOAC), low linolenic acid canola (LLAC) and high erucic acid rapeseed (HEAR). Controlled experiments were conducted to evaluate the, effects of water deficit and temperature stress on fatty acid composition of conventional canola, HOAC, LLAC and HEAR. Ten years of data from the advanced canola trials of Agriculture Victoria were analysed, and a three-year trial was undertaken to identify the south-eastern Australian locations which produced specialty cultivars with the highest oil and seed protein content, lowest glucosinolate concentration, and premium oil quality (i.e. most appropriate fatty acid composition). Two glasshouse trials were performed to determine the separate effects of severe water deficit and three days of high (32C) and very high temperatures (37C) at 10, 20 and 30 days after flowering (DAF) on the oil composition of a HEAR cultivar and HOAC cultivar. In contrast to earlier work on the effects of sustained high postflowering temperatures, three hot days had no effect on oil composition or yield of the two cultivars, except that 37/25C (day/night) from 30-33 DAF reduced erucic acid content of HEAR from 52% to 44%. Severe drought reduced the quality and yields of both cultivars. Erucic acid content of the HEAR cultivar decreased by up to 10% and the oleic acid content decreased by up to 4% with post-flowering drought. Within each year, most variation in the quality components of the three year field trial was due to site. Oil ,content and seed yield were highest, and seed protein content and glucosinolate concentration were lowest, in the cooler and wetter sites and years. Generally, warmer post-flowering conditions enhanced oleic acid content at the expense of linoleic and linolenic acids in the canola quality cultivars, although all regressions between quality and weather variables were fairly weak. In 1995, for. every 5C increase in the average temperature between flowering and maturity, oil content decreased and seed protein content increased by an average 4%, glucosinolate concentration increased by 4.0 ?mol/g, and the linoleic and linolenic acid content each decreased by 1-2% in the different canola quality types. Each 100 mm increase in rainfall between flowering and maturity increased yields by 0.4 t/ha and oil content by 1.6%, and reduced seed protein content by 0.5% and glucosinolate concentration by 1.1 ?mol/g, on average. Oil content was strongly negatively correlated with seed protein content (r=-0.75 for conventional canola). Upon removing the effects of year and cultivar from the ten year dataset, the mean oleic acid content, of conventional canota was very stable across regions, but varied more between years. Canola grown in - central Victoria and the Wimmera produced consistently high levels (>60%) of oleic acid. Canola consistently achieved a low linolenic acid content in central Victoria (<10.8%), and a moderate to low: linolenic acid content in the Wimmera. The Mallee produced canola with highly variable levels of oleic acid and linolenic acid, despite warm post-flowering temperatures which are known to increase oleic acid content. In the three year trial, time of sowing did not have a consistent effect. on the fatty acid composition of canola, probably due to the interactions between temperature and rainfall. These results identified central Victoria and the Wimmera as the most appropriate locations for the production of HOAC and LLAC cultivars. One year of data indicated that Wagga Wagga is capable of producing canola with extremely high oleic acid and low linolenic acid levels. The results of the three year trial identified lower south-eastern South Australia and the Victorian Wimmera as regions most conducive to the production of HEAR with consistently high levels of erucic acid. The erucic acid content of HEAR was often reduced by late sowing. However, this trend was not always observed, and possible reasons for deviations from trends have been discussed. One year of data demonstrated high levels of erucic acid in HEAR produced in the southern part of central Victoria. As the time of sowing did not have a consistent effect on the erucic acid content of HEAR, or the oleic and linolenic acid content of HOAC and LLAC, it was recommended that growers sow at the same time as recommended for conventional canola, to maximise yield and oil content.
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ItemPhysiological aspects of root growth of Eucalyptus pauciflora, subsp. pauciflora and Eucalyptus nitens( 1997)This thesis examined i) morphological and physiological effects of low soil temperatures on root growth of subalpine Eucalyptus pauciflora Sieber ex Sprengel subsp. pauciflora and montane Eucalyptus nitens (Deane & Maiden) Maiden, ii) determined the variability, and in particular the day/night variability, in root elongation, and iii) explored the physiological basis for such variability. A series of experiments were undertaken with seedlings of E. pauciflora and E. nitens grown at soil temperatures of 3, 7, and 13C, and where seedlings were transferred from one temperature to another. E. nitens grew faster than E. pauciflora at 7 and 13C, but E. pauciflora grew faster than E. nitens at 3C. E. pauciflora always produced greater total and white root length than E. nitens. E. nitens roots browned faster in response to lowering soil temperatures than E. pauciflora. The osmotic potential of the roots decreased with soil temperature, but more so in E. pauciflora than E. nitens. Proline was a prominent osmoregulant in roots of E. pauciflora and arginine in E. nitens roots. It is suggested that E. pauciflora is better adapted than E. nitens to root growth at low soil temperatures because it can keep roots white longer and can maintain lower root osmotic potentials. Root growth of E. pauciflora was examined for 31 months (December 1992 - June 1995) in a mature stand at an elevation of 1545 m on Mt Stirling, Victoria, Australia. Greater night than day root elongation was recorded from eight in situ rhizotrons during the summer and early autumn of 1993. Shoot growth was also monitored during part of this study (April 1994 - June 1995). It was found that root growth commenced in the spring at soil temperatures 5 1.5C, under 550 mm of snow, at least one month before the onset of shoot growth and continued at least two months longer that shoot growth during the autumn. A period of root dormancy for at least one month a year occurred in roots of E. pauciflora. The seasonal variability in root numbers of E. pauciflora appeared to be related mainly to soil temperature and to a lesser extent to soil water content. Moreover, there appeared to be some internal periodicity in root growth which was independent of the external environment on Mt Stirling. Greater night than day root elongation was recorded in seedlings of both eucalypts in a glasshouse. Root elongation rates were greatest in E. nitens, and root elongation of both eucalypt seedlings were greater than that of the mature E. pauciflora on Mt Stirling. The zones of day and night elongation were determined in root marking experiments. Histological studies of the zone of elongation showed that cell division occurred mainly during the day and cell elongation mainly at night. Night root elongation rates were increased by increasing day-time air temperatures, light-period, and light intensity; and by decreasing water stress during the night. The turgor pressure of the root tips was greater during the night than the day. It is suggested that the amount of root growth during the night is determined directly by turgor pressure during the night and indirectly by processes during the day (light duration and intensity, and temperature during the light period) which determine the extent of cell division during the day. A greater rate of cell division during the day will be translated into a greater rate of root elongation, especially in the night.
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ItemControl of vegetative vigour of peach orchards( 1996)In the Goulburn/Murray Valley of South-Eastern Australia, orchard management aims to maximise productivity and ensure sustainability. Achievement of this objective relies on the control of excess vegetative vigour, minimisation of salinity and waterlogging damage and improvement of irrigation efficiency. The overall aim of this study was to identify the major controls over vegetative vigour under varying environmental conditions with a view to providing practical management strategies. Options which are currently available for control of vegetative vigour include dwarfing rootstocks, chemical growth regulators and summer pruning. Alternative systems which may be more suitable are Regulated Deficit Irrigation (RDI), saline irrigation and Restricted Root Volume (RRV). Regulated Deficit Irrigation (RDI) applied in a high-density peach orchard controlled vegetative growth (reduction of 50%) and maintained yield. RDI trees used less water than traditionally irrigated trees during the RDI period. This was attributed to reduced water availability and plant water status. Differences in water use continued after RDI due to a combination of tree size, leaf area and micro-advection. Irrigation with water of EC greater than 0.5 dS m-1 combined with RDI, reduced vegetative and fruit growth, yield and tree water use. Yield declined as a consequence of smaller leaf area, decreased photosynthetic activity and overall poor tree health. The adverse effects were largely attributed to chloride toxicity with leaf CI concentration approaching 3% dry matter. In a saline environment, RDI will require the inclusion of strategic leaching. A shallow non-saline water table combined with RDI initially enhanced vegetative growth. However, in the second season, adverse effects of both non-saline and saline water tables on tree growth and productivity were demonstrated. Tree water use was initially greater over the non-saline water table which contributed approximately 28% of total water used. Chloride concentrated in the leaves and fruit, while Na concentrated in the bark, butt wood and structural roots. These results demonstrate the need for both leaching of the soil and limiting drainage to the water table. Artificial drainage and/or accurate irrigation scheduling will be essential features of successful management. Restricted root volume (RRV) reduced tree growth although the effect decreased in the third and fourth seasons. Water stress (RDI) had little effect on tree growth despite considerable differences in plant water status. Trees in the smallest soil volume demonstrated difficulty in achieving adequate fruit size. There were significant differences in water use in response to soil volume and RDI management. Water use followed similar seasonal patterns as previously established. In summary, RDI is a viable management option for the control of vegetative growth in high density peach orchards and RRV is effective in controlling vegetative growth. More research is required, however, before RRV can be adopted by growers. Saline irrigation reduced vegetative growth and also productivity and is therefore not considered an option for control of vegetative vigour. Salinity and water tables should be managed to minimise their effect.
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ItemEffects of post-anthesis stress on grain filling and malting quality of barley( 1995)Malting quality is variable from year to year in many countries with Mediterranean or continental climates. Environmental stresses are thought to contribute to that variability. However, little information is available on the relationships between environmental conditions during grain growth and malting quality. Two of the most frequent environmental constraints during grain filling in many cereal-growing areas of the world are high temperature and drought. Short periods (ca. 5 days) of very high maximum temperature (>35C) are quite common during grain growth and have been identified as a potential source of variation in malting quality. Therefore, the main objectives of the present study, were to determine the effect of short periods of high temperature and drought on grain growth and malting quality of barley. The study involved seven experiments, in which short periods (5 days) of high temperature and drought, combined or alone, were imposed during grain filling on the malting cultivar Schooner. Where possible, other malting cultivars were included (Chapters 2 and 3). The effects of short periods of high temperature on grain yield and malting quality of barley were first assessed under field conditions using portable chambers with thermostatically-controlled electric heaters. High temperature imposed for 5 days (17 to 21 days after anthesis) with a maximum temperature of 40C maintained for 6 h per day reduced grain weight by 13% in Schooner and 25% in Parwan. There was a reduction in starch content and an increase in nitrogen content in the heat treatments, but B-glucan content was not affected. High temperature reduced the amount of 'maltable' grain by reducing grain size and increasing screening percentage, and also reduced malt extract by 3-7%, which represents a large decrease for the malting industry. The other experiments in this thesis were carried out under controlled-environment conditions, in order to overcome difficulties of temperature and humidity control. Short periods of high temperature were imposed for 5 or 10 days at mid-grain filling on Schooner and Franklin, with or without drought treatments. Short periods of high temperature reduced grain weight by 5%, while drought reduced it by 20%. High temperature and drought together resulted in the greatest reduction (30%). There was a reduction in starch content and an increase in diastatic power and ?-glucan degradation under stress. However, malt extract was not significantly affected. To determine the importance of timing of short periods of high temperature and drought on grain weight and malting quality, a glasshouse experiment was carried out in which Schooner barley was exposed to these stresses at early, mid or late grain filling. Individual grain weight was most sensitive to high temperature and drought treatments imposed early in grain filling (10-15 days after anthesis) and was less sensitive to later treatments. Starch was reduced in amount and quality, especially with early stresses during grain filling. However, malt extract was not significantly affected. Finally, two experiments were carried out in the Canberra phytotron to study the effects of the temperature regime before and after heat stress on grain growth and quality. In the first experiment, the hypothesis that under a gradual increase in temperature, plants could develop some acclimation was tested. Plants experiencing either a sudden or a gradual increase did not exhibit any differences in grain weight or malting quality, but increasing the temperature in two steps (so that plants were exposed to 30 or 34C for 2 h before a 40C heat stress), appeared to have produced acclimation, since the reduction in grain weight under the two step treatment was about half that of either sudden or gradual increase in temperature. In the second experiment, the hypothesis tested was that grain growth would recover better from short stress under cool (21/16C) than warm (27/22C and 30/250 conditions following that heat stress. The reduction in yield caused by heat stress was not alleviated by the succeeding moderately high temperatures. The following conclusions were derived from this study: (i) the reduction in grain weight ranged from 5 to 35% in response to short periods of high temperature and drought during grain filling in barley. The magnitude of the reduction depended on duration and timing of exposure, (ii) the reduction in grain weight was accompanied by an increase in screening percentage corresponding to a large reduction in amount of 'maltable grain', (iii) grain composition was altered by these stresses, and in general, starch content was most affected. There was a strong and positive relationship between the reduction in grain weight and starch content per grain (R2=0.92, P<0.001). In all the experiments, there were reductions in the volumes of both A- and B-type starch granules; however, the reduction in grain weight was mostly closely related to the reduction in the volume of Atype starch granules. The stress-induced increase in nitrogen percentage was smaller than expected, probably because post-anthesis availability of nitrogen was less limited than under typical field conditions. Grain ?-glucan content tended to be reduced under drought but there was no clear trend under heat stress, and (iv) malt extract was not highly responsive in any of the high temperature or drought experiments. Malt extract was reduced by 3 to 7% in the field experiments (Chapter 2) and by 5% in a glasshouse experiment (Chapter 5) with short periods of heat stress. Although small relative to the grain yield reductions observed, such changes in malt extract are large for the malting industry. High temperature and drought affected several components of malting quality in opposing directions, for example the stresses reduced starch content, which would tend to reduce malt extract but also tended to decrease ?-glucan and increase diastatic power which would tend to increase malt extract. The net result of these opposing changes was generally a minor effect of heat stress and drought on malt extract, even though the main quality components contributing to malt extract often strongly responded to these stresses.
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