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dc.contributor.authorRobb, E
dc.contributor.authorPerez, K
dc.contributor.authorHung, LW
dc.contributor.authorMasters, CL
dc.contributor.authorBarnham, KJ
dc.contributor.authorCherny, RA
dc.contributor.authorBush, AI
dc.contributor.authorAdlard, PA
dc.contributor.authorFinkelstein, DI
dc.date.available2016-08-11T01:35:32Z
dc.date.issued2015-01-01
dc.identifierpii: V8318J8405651185
dc.identifier.citationRobb, E., Perez, K., Hung, L. W., Masters, C. L., Barnham, K. J., Cherny, R. A., Bush, A. I., Adlard, P. A. & Finkelstein, D. I. (2015). High Order W02-Reactive Stable Oligomers of Amyloid-beta are Produced in vivo and in vitro via Dialysis and Filtration of Synthetic Amyloid-beta Monomer. JOURNAL OF ALZHEIMERS DISEASE, 44 (1), pp.69-78. https://doi.org/10.3233/JAD-132024.
dc.identifier.issn1387-2877
dc.identifier.urihttp://hdl.handle.net/11343/113667
dc.description.abstractOligomeric forms of amyloid-β (Aβ) are thought to be responsible for the pathogenesis of Alzheimer's disease. While many oligomers of Aβ are thought to be naturally occurring in the brain of humans and/or transgenic animals, it is well known that Aβ oligomers are also readily produced in vitro in the laboratory. In recent studies, we discovered that synthetic monomeric Aβ (4.7 kDa) could be transformed by microdialysis to higher molecular weight species (approximately 56 kDa, by western blot). Surface-enhanced laser desorption/ionization mass spectrometry and electron microscopy further identified these species' as potential Aβ oligomers. The production of similar species could also be produced by centrifugal filtration and this formation was concentration and pore-size dependent. These higher order species of Aβ were resistant to dissolution in NaOH, HFIP, formic acid, urea, and guanidine. We postulate that we have identified a novel way of producing a high order species of oligomeric Aβ and we provide evidence to suggest that Aβ oligomers can quite easily be a product of normal laboratory practices. These data suggest that the experimental detection of higher order oligomers in tissues derived from Alzheimer's disease brains or from animal models of disease could, in some cases, be a product the method of analysis.
dc.languageEnglish
dc.publisherIOS PRESS
dc.titleHigh Order W02-Reactive Stable Oligomers of Amyloid-beta are Produced in vivo and in vitro via Dialysis and Filtration of Synthetic Amyloid-beta Monomer
dc.typeJournal Article
dc.identifier.doi10.3233/JAD-132024
melbourne.affiliation.departmentFlorey Department of Neuroscience and Mental Health
melbourne.affiliation.departmentCentre for Neuroscience
melbourne.affiliation.departmentPharmacology and Therapeutics
melbourne.source.titleJOURNAL OF ALZHEIMERS DISEASE
melbourne.source.volume44
melbourne.source.issue1
melbourne.source.pages69-78
melbourne.identifier.nhmrc1037234
melbourne.identifier.nhmrc509286
melbourne.identifier.nhmrc1044542
melbourne.identifier.nhmrc1043992
melbourne.elementsid923573
melbourne.contributor.authorMasters, Colin
melbourne.contributor.authorBush, Ashley
melbourne.contributor.authorFinkelstein, David
melbourne.contributor.authorBarnham, Kevin
melbourne.contributor.authorROBB, ELYSIA
melbourne.contributor.authorPerez, Keyla
dc.identifier.eissn1875-8908
melbourne.identifier.fundernameidNHMRC, 1037234
melbourne.identifier.fundernameidNHMRC, 509286
melbourne.identifier.fundernameidNHMRC, 1044542
melbourne.identifier.fundernameidNHMRC, 1043992
melbourne.accessrightsThis item is currently not available from this repository


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