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dc.contributor.authorMaster, JS
dc.contributor.authorThouas, GA
dc.contributor.authorHarvey, AJ
dc.contributor.authorSheedy, JR
dc.contributor.authorHannan, NJ
dc.contributor.authorGardner, DK
dc.contributor.authorWlodek, ME
dc.date.available2016-09-22T07:31:14Z
dc.date.issued2015-05-01
dc.identifierpii: REP-14-0619
dc.identifier.citationMaster, J. S., Thouas, G. A., Harvey, A. J., Sheedy, J. R., Hannan, N. J., Gardner, D. K. & Wlodek, M. E. (2015). Low female birth weight and advanced maternal age programme alterations in next-generation blastocyst development. REPRODUCTION, 149 (5), pp.497-510. https://doi.org/10.1530/REP-14-0619.
dc.identifier.issn1470-1626
dc.identifier.urihttp://hdl.handle.net/11343/116577
dc.descriptionThe Accepted version attached has the title 'Low female birth weight and advanced maternal age program alterations in next generation blastocyst development'.
dc.description.abstractLow birth weight is associated with an increased risk for adult disease development with recent studies highlighting transmission to subsequent generations. However, the mechanisms and timing of programming of disease transmission to the next generation remain unknown. The aim of this study was to examine the effects of low birth weight and advanced maternal age on second-generation preimplantation blastocysts. Uteroplacental insufficiency or sham surgery was performed in late-gestation WKY pregnant rats, giving rise to first-generation (F1) restricted (born small) and control offspring respectively. F1 control and restricted females, at 4 or 12 months of age, were naturally mated with normal males. Second-generation (F2) blastocysts from restricted females displayed reduced expression of genes related to growth compared with F2 control (P<0.05). Following 24 h culture, F2 restricted blastocysts had accelerated development, with increased total cell number, a result of increased trophectoderm cells compared with control (P<0.05). There were alterations in carbohydrate and serine utilisation in F2 restricted blastocysts and F2 restricted outgrowths from 4-month-old females respectively (P<0.05). F2 blastocysts from aged restricted females were developmentally delayed at retrieval, with reduced total cell number attributable to reduced trophectoderm number with changes in carbohydrate utilisation (P<0.05). Advanced maternal age resulted in alterations in a number of amino acids in media obtained from F2 blastocyst outgrowths (P<0.05). These findings demonstrate that growth restriction and advanced maternal age can alter F2 preimplantation embryo physiology and the subsequent offspring growth.
dc.languageEnglish
dc.publisherBIOSCIENTIFICA LTD
dc.titleLow female birth weight and advanced maternal age programme alterations in next-generation blastocyst development
dc.typeJournal Article
dc.identifier.doi10.1530/REP-14-0619
melbourne.affiliation.departmentObstetrics and Gynaecology
melbourne.affiliation.departmentPhysiology
melbourne.affiliation.departmentSchool of BioSciences
melbourne.affiliation.departmentZoology
melbourne.source.titleREPRODUCTION
melbourne.source.volume149
melbourne.source.issue5
melbourne.source.pages497-510
melbourne.identifier.nhmrc1045602
melbourne.identifier.nhmrc628927
melbourne.elementsid947962
melbourne.contributor.authorHarvey, Alexandra
melbourne.contributor.authorSHEEDY, JOHN
melbourne.contributor.authorGardner, David
melbourne.contributor.authorHannan, Natalie
melbourne.contributor.authorMaster, Jordanna
melbourne.contributor.authorWlodek, Mary
melbourne.contributor.authorTHOUAS, GEORGE
dc.identifier.eissn1741-7899
melbourne.identifier.fundernameidNHMRC, 1045602
melbourne.identifier.fundernameidNHMRC, 628927
melbourne.accessrightsOpen Access


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