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dc.contributor.authorWong, W
dc.contributor.authorBai, X-C
dc.contributor.authorBrown, A
dc.contributor.authorFernandez, IS
dc.contributor.authorHanssen, E
dc.contributor.authorCondron, M
dc.contributor.authorTan, YH
dc.contributor.authorBaum, J
dc.contributor.authorScheres, SHW
dc.date.available2016-10-31T04:14:58Z
dc.date.available2014-06-06
dc.date.available2014-06-06
dc.date.available2014-06-06
dc.date.available2014-06-06
dc.date.issued2014-06-09
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000338716500001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=d4d813f4571fa7d6246bdc0dfeca3a1c
dc.identifierARTN e03080
dc.identifier.citationWong, W., Bai, X. -C., Brown, A., Fernandez, I. S., Hanssen, E., Condron, M., Tan, Y. H., Baum, J. & Scheres, S. H. W. (2014). Cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetine. ELIFE, 3 (3), https://doi.org/10.7554/eLife.03080.
dc.identifier.issn2050-084X
dc.identifier.urihttp://hdl.handle.net/11343/118712
dc.description.abstractMalaria inflicts an enormous burden on global human health. The emergence of parasite resistance to front-line drugs has prompted a renewed focus on the repositioning of clinically approved drugs as potential anti-malarial therapies. Antibiotics that inhibit protein translation are promising candidates for repositioning. We have solved the cryo-EM structure of the cytoplasmic ribosome from the human malaria parasite, Plasmodium falciparum, in complex with emetine at 3.2 Å resolution. Emetine is an anti-protozoan drug used in the treatment of ameobiasis that also displays potent anti-malarial activity. Emetine interacts with the E-site of the ribosomal small subunit and shares a similar binding site with the antibiotic pactamycin, thereby delivering its therapeutic effect by blocking mRNA/tRNA translocation. As the first cryo-EM structure that visualizes an antibiotic bound to any ribosome at atomic resolution, this establishes cryo-EM as a powerful tool for screening and guiding the design of drugs that target parasite translation machinery.
dc.languageEnglish
dc.publisherELIFE SCIENCES PUBLICATIONS LTD
dc.titleCryo-EM structure of the Plasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetine
dc.typeJournal Article
dc.identifier.doi10.7554/eLife.03080
melbourne.affiliation.departmentBio21
melbourne.affiliation.departmentMedical Biology
melbourne.affiliation.departmentMedicine, Dentistry & Health Sciences
melbourne.affiliation.departmentMicrobiology & Immunology
melbourne.affiliation.departmentMelbourne Students & Learning
melbourne.source.titleELIFE
melbourne.source.volume3
melbourne.source.issue3
dc.rights.licenseCC BY
melbourne.elementsid896245
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4086275
melbourne.contributor.authorTan, Yan Hong
melbourne.contributor.authorBAUM, JACOB
melbourne.contributor.authorHanssen, Eric
melbourne.contributor.authorWong, Wilson
melbourne.contributor.authorBAI, XIAOCHEN
dc.identifier.eissn2050-084X
pubs.acceptance.date2014-06-06
melbourne.accessrightsOpen Access


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