Improving high resolution retinal image quality using speckle illumination HiLo imaging
AuthorZhou, X; Bedggood, P; Metha, A
Source TitleBIOMEDICAL OPTICS EXPRESS
PublisherOPTICAL SOC AMER
AffiliationMelbourne School of Health Sciences
Optometry and Vision Sciences
Document TypeJournal Article
CitationsZhou, X; Bedggood, P; Metha, A, Improving high resolution retinal image quality using speckle illumination HiLo imaging, BIOMEDICAL OPTICS EXPRESS, 2014, 5 (8), pp. 2563 - 2579
Access StatusOpen Access
Open Access at PMChttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4132989
Retinal image quality from flood illumination adaptive optics (AO) ophthalmoscopes is adversely affected by out-of-focus light scatter due to the lack of confocality. This effect is more pronounced in small eyes, such as that of rodents, because the requisite high optical power confers a large dioptric thickness to the retina. A recently-developed structured illumination microscopy (SIM) technique called HiLo imaging has been shown to reduce the effect of out-of-focus light scatter in flood illumination microscopes and produce pseudo-confocal images with significantly improved image quality. In this work, we adopted the HiLo technique to a flood AO ophthalmoscope and performed AO imaging in both (physical) model and live rat eyes. The improvement in image quality from HiLo imaging is shown both qualitatively and quantitatively by using spatial spectral analysis.
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