Enriched retinal ganglion cells derived from human embryonic stem cells
AuthorGill, KP; Hung, SSC; Sharov, A; Lo, CY; Needham, K; Lidgerwood, GE; Jackson, S; Crombie, DE; Nayagam, BA; Cook, AL; ...
Source TitleSCIENTIFIC REPORTS
PublisherNATURE PUBLISHING GROUP
University of Melbourne Author/sNeedham, Karina; Nayagam, Bryony; Pebay, Alice; Lidgerwood, Grace; Wong, Raymond; HEWITT, ALEXANDER; Crombie, Duncan; Gill, Katherine; Hung, Sandy; Lidgerwood, Grace; ...
Ophthalmology (Eye & Ear Hospital)
Pharmacology and Therapeutics
Audiology and Speech Pathology
Centre for Eye Research Australia (CERA)
Document TypeJournal Article
CitationsGill, KP; Hung, SSC; Sharov, A; Lo, CY; Needham, K; Lidgerwood, GE; Jackson, S; Crombie, DE; Nayagam, BA; Cook, AL; Hewitt, AW; Pebay, A; Wong, RCB, Enriched retinal ganglion cells derived from human embryonic stem cells, SCIENTIFIC REPORTS, 2016, 6
Access StatusOpen Access
Open Access at PMChttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4978994
ARC Grant codeARC/FT140100047
Optic neuropathies are characterised by a loss of retinal ganglion cells (RGCs) that lead to vision impairment. Development of cell therapy requires a better understanding of the signals that direct stem cells into RGCs. Human embryonic stem cells (hESCs) represent an unlimited cellular source for generation of human RGCs in vitro. In this study, we present a 45-day protocol that utilises magnetic activated cell sorting to generate enriched population of RGCs via stepwise retinal differentiation using hESCs. We performed an extensive characterization of these stem cell-derived RGCs by examining the gene and protein expressions of a panel of neural/RGC markers. Furthermore, whole transcriptome analysis demonstrated similarity of the hESC-derived RGCs to human adult RGCs. The enriched hESC-RGCs possess long axons, functional electrophysiological profiles and axonal transport of mitochondria, suggestive of maturity. In summary, this RGC differentiation protocol can generate an enriched population of functional RGCs from hESCs, allowing future studies on disease modeling of optic neuropathies and development of cell therapies.
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