Glucagon-like peptide 1 and peptide YY are in separate storage organelles in enteroendocrine cells
AuthorCho, H-J; Robinson, ES; Rivera, LR; McMillan, PJ; Testro, A; Nikfarjam, M; Bravo, DM; Furness, JB
Source TitleCell and Tissue Research
University of Melbourne Author/sFurness, John; Cho, Hyun-Jung; McMillan, Paul; Nikfarjam, Mehrdad; Testro, Adam; RIVERA, LENI; ROBINSON, ELIZA
AffiliationClinical School (Austin Health)
Surgery (Austin & Northern Health)
Anatomy and Neuroscience
Biochemistry and Molecular Biology
Document TypeJournal Article
CitationsCho, H. -J., Robinson, E. S., Rivera, L. R., McMillan, P. J., Testro, A., Nikfarjam, M., Bravo, D. M. & Furness, J. B. (2014). Glucagon-like peptide 1 and peptide YY are in separate storage organelles in enteroendocrine cells. CELL AND TISSUE RESEARCH, 357 (1), pp.63-69. https://doi.org/10.1007/s00441-014-1886-9.
Access StatusOpen Access
A sub-group of enteroendocrine cells (L cells) release gastrointestinal hormones, GLP-1 and PYY, which have different but overlapping physiological effects, in response to intraluminal nutrients. Whilst their release profiles are not identical, how the plasma levels of these two hormones are differentially regulated is not well understood. We investigate the possibility that GLP-1 and PYY are in separate storage vesicles. In this study, the subcellular location of GLP-1 and PYY storage organelles is investigated using double-labelling immunohistochemistry, super resolution microscopy and high-resolution confocal microscopy. In all species tested, human, pig, rat and mouse, most cytoplasmic stores that exhibited GLP-1 or PYY immunofluorescence were distinct from each other. The volume occupancy, determined by 3D analysis, overlapped by only about 10∼20 %. At the lower resolution achieved by conventional confocal microscopy, there was also evidence of GLP-1 and PYY being in separate storage compartments but, in subcellular regions where there were many storage vesicles, separate storage could not be resolved. The results indicate that different storage vesicles in L cells contain predominantly GLP-1 or predominantly PYY. Whether GLP-1 and PYY storage vesicles are selectively mobilised and their products are selectively released needs to be determined.
- Click on "Export Reference in RIS Format" and choose "open with... Endnote".
- Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References