Understanding the mechanism of the synthesis of β-D-(1,3;1,4)-glucans, or mixed linkage glucans (MLGs), by the cellulose synthase like (CSL) F6 protein
AffiliationSchool of Botany
Document TypePhD thesis
Access StatusThis item is embargoed and will be available on 2021-01-31. This item is currently available to University of Melbourne staff and students only, login required.
© 2018 Dr. Melissa Bain
The molecular mechanism by which mixed linkage, (1,3;1,4)-β-D-glucan, a non-cellulosic polysaccharide, is synthesised by cellulose synthase-like (CSL) F6 was explored. CSLF6 was shown to be crucial for development in the proposed model grass Brachypodium distachyon through analysis of point mutants. In silico homology modelling of related β-glucan synthases was used to predict domains influencing specificity, informing targeted mutagenesis. CSLF6 from Lolium multiflorum expressed in Escherichia coli was found to produce (1,3;1,4)-β-D-glucan similar to native sources in vitro, allowing biochemical characterisation.
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