University Library
  • Login
A gateway to Melbourne's research publications
Minerva Access is the University's Institutional Repository. It aims to collect, preserve, and showcase the intellectual output of staff and students of the University of Melbourne for a global audience.
View Item 
  • Minerva Access
  • Medicine, Dentistry & Health Sciences
  • Medical Biology
  • Medical Biology - Research Publications
  • View Item
  • Minerva Access
  • Medicine, Dentistry & Health Sciences
  • Medical Biology
  • Medical Biology - Research Publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

    The &ITPlasmodium falciparum &ITtranscriptome in severe malaria reveals altered expression of genes involved in important processes including surface antigen-encoding &ITvar &ITgenes

    Thumbnail
    Download
    Published version (14.85Mb)

    Citations
    Scopus
    Web of Science
    Altmetric
    23
    19
    Author
    Tonkin-Hill, GQ; Trianty, L; Noviyanti, R; Nguyen, HHT; Sebayang, BF; Lampah, DA; Marfurt, J; Cobbold, SA; Rambhatla, JS; McConville, MJ; ...
    Date
    2018-03-01
    Source Title
    PLOS BIOLOGY
    Publisher
    PUBLIC LIBRARY SCIENCE
    University of Melbourne Author/s
    Cobbold, Simon; McConville, Malcolm; Duffy, Michael; Rogerson, Stephen; Brown, Graham; Papenfuss, Anthony; Nguyen, Thi Hong Hanh; Rambhatla, Janavi Suresh; Day, Karen
    Affiliation
    Medical Biology (W.E.H.I.)
    Medicine and Radiology
    Medicine (RMH)
    Clinical School (Royal Melbourne Hospital)
    Melbourne School of Population and Global Health
    Biochemistry and Molecular Biology
    Microbiology and Immunology
    School of BioSciences
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Tonkin-Hill, G. Q., Trianty, L., Noviyanti, R., Nguyen, H. H. T., Sebayang, B. F., Lampah, D. A., Marfurt, J., Cobbold, S. A., Rambhatla, J. S., McConville, M. J., Rogerson, S. J., Brown, G., Day, K. P., Price, R. N., Anstey, N. M., Papenfuss, A. T. & Duffy, M. F. (2018). The &ITPlasmodium falciparum &ITtranscriptome in severe malaria reveals altered expression of genes involved in important processes including surface antigen-encoding &ITvar &ITgenes. PLOS BIOLOGY, 16 (3), https://doi.org/10.1371/journal.pbio.2004328.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/227644
    DOI
    10.1371/journal.pbio.2004328
    Open Access at PMC
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5864071
    NHMRC Grant code
    NHMRC/1007954
    Abstract
    Within the human host, the malaria parasite Plasmodium falciparum is exposed to multiple selection pressures. The host environment changes dramatically in severe malaria, but the extent to which the parasite responds to-or is selected by-this environment remains unclear. From previous studies, the parasites that cause severe malaria appear to increase expression of a restricted but poorly defined subset of the PfEMP1 variant, surface antigens. PfEMP1s are major targets of protective immunity. Here, we used RNA sequencing (RNAseq) to analyse gene expression in 44 parasite isolates that caused severe and uncomplicated malaria in Papuan patients. The transcriptomes of 19 parasite isolates associated with severe malaria indicated that these parasites had decreased glycolysis without activation of compensatory pathways; altered chromatin structure and probably transcriptional regulation through decreased histone methylation; reduced surface expression of PfEMP1; and down-regulated expression of multiple chaperone proteins. Our RNAseq also identified novel associations between disease severity and PfEMP1 transcripts, domains, and smaller sequence segments and also confirmed all previously reported associations between expressed PfEMP1 sequences and severe disease. These findings will inform efforts to identify vaccine targets for severe malaria and also indicate how parasites adapt to-or are selected by-the host environment in severe malaria.

    Export Reference in RIS Format     

    Endnote

    • Click on "Export Reference in RIS Format" and choose "open with... Endnote".

    Refworks

    • Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References


    Collections
    • Minerva Elements Records [45689]
    • School of BioSciences - Research Publications [1092]
    • Microbiology & Immunology - Research Publications [1555]
    • Biochemistry and Molecular Biology - Research Publications [787]
    • Melbourne School of Population and Global Health - Research Publications [4369]
    • Clinical School (Royal Melbourne Hospital) - Research Publications [16]
    • Medicine (RMH) - Research Publications [786]
    • Medicine and Radiology - Research Publications [2346]
    • Medical Biology - Research Publications [865]
    Minerva AccessDepositing Your Work (for University of Melbourne Staff and Students)NewsFAQs

    BrowseCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects
    My AccountLoginRegister
    StatisticsMost Popular ItemsStatistics by CountryMost Popular Authors