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dc.contributor.authorChan, J
dc.contributor.authorSoraya, GV
dc.contributor.authorCraig, L
dc.contributor.authorUddin, SM
dc.contributor.authorTodaro, M
dc.contributor.authorHuynh, DH
dc.contributor.authorAbeyrathne, CD
dc.contributor.authorKostenko, L
dc.contributor.authorMcCluskey, J
dc.contributor.authorSkafidas, E
dc.contributor.authorKwan, P
dc.date.available2019-10-16T10:43:19Z
dc.date.available2019-08-11
dc.date.available2019-08-11
dc.date.issued2019-08-20
dc.identifierpii: s19163543
dc.identifier.citationChan, J., Soraya, G. V., Craig, L., Uddin, S. M., Todaro, M., Huynh, D. H., Abeyrathne, C. D., Kostenko, L., McCluskey, J., Skafidas, E. & Kwan, P. (2019). Rapid Detection of HLA-B*57:01-Expressing Cells Using a Label-Free Interdigitated Electrode Biosensor Platform for Prevention of Abacavir Hypersensitivity in HIV Treatment. Sensors, 19 (16), https://doi.org/10.3390/s19163543.
dc.identifier.issn1424-8220
dc.identifier.urihttp://hdl.handle.net/11343/229630
dc.description.abstractPre-treatment screening of individuals for human leukocyte antigens (HLA) HLA-B*57:01 is recommended for the prevention of life-threatening hypersensitivity reactions to abacavir, a drug widely prescribed for HIV treatment. However, the implementation of screening in clinical practice is hindered by the slow turnaround time and high cost of conventional HLA genotyping methods. We have developed a biosensor platform using interdigitated electrode (IDE) functionalized with a monoclonal antibody to detect cells expressing HLA-B*57:01. This platform was evaluated using cell lines and peripheral blood mononuclear cells expressing different HLA-B alleles. The functionalized IDE sensor was able to specifically capture HLA-B*57:01 cells, resulting in a significant change in the impedance magnitude in 20 min. This IDE platform has the potential to be further developed to enable point-of-care HLA-B*57:01 screening
dc.languageEnglish
dc.publisherMDPI AG
dc.titleRapid Detection of HLA-B*57:01-Expressing Cells Using a Label-Free Interdigitated Electrode Biosensor Platform for Prevention of Abacavir Hypersensitivity in HIV Treatment
dc.typeJournal Article
dc.identifier.doi10.3390/s19163543
melbourne.affiliation.departmentMedicine and Radiology
melbourne.affiliation.departmentMedicine (RMH)
melbourne.affiliation.departmentElectrical and Electronic Engineering
melbourne.affiliation.departmentChancellery Research
melbourne.source.titleSensors
melbourne.source.volume19
melbourne.source.issue16
melbourne.identifier.arcDP170103822
dc.rights.licenseCC BY
melbourne.elementsid1407883
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC6719006
melbourne.contributor.authorMcCluskey, James
melbourne.contributor.authorTodaro, Marian
melbourne.contributor.authorSkafidas, Efstratios
melbourne.contributor.authorKwan, Patrick
melbourne.contributor.authorKostenko, Lyudmila
melbourne.contributor.authorChan, Jianxiong
melbourne.contributor.authorAbeyrathne, Chathurika
melbourne.contributor.authorSoraya, Gita
melbourne.contributor.authorCraig, Lauren Amy
melbourne.contributor.authorUddin, Shah Mukim
melbourne.contributor.authorHuynh, Duc Hau
dc.identifier.eissn1424-8220
melbourne.identifier.fundernameidDP170103822
pubs.acceptance.date2019-08-11
melbourne.accessrightsOpen Access


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