Diverse MR1-restricted T cells in mice and humans.
AuthorKoay, H-F; Gherardin, NA; Xu, C; Seneviratna, R; Zhao, Z; Chen, Z; Fairlie, DP; McCluskey, J; Pellicci, DG; Uldrich, AP; ...
Source TitleNature Communications
PublisherNature Research (part of Springer Nature)
University of Melbourne Author/sKoay, Hui Fern; Chen, Zhenjun; McCluskey, James; Godfrey, Dale; Pellicci, Daniel; Uldrich, Adam; Gherardin, Nicholas; Xu, Calvin; Seneviratna, Rebecca; Zhao, Zhe
AffiliationMicrobiology and Immunology
Document TypeJournal Article
CitationsKoay, H. -F., Gherardin, N. A., Xu, C., Seneviratna, R., Zhao, Z., Chen, Z., Fairlie, D. P., McCluskey, J., Pellicci, D. G., Uldrich, A. P. & Godfrey, D. I. (2019). Diverse MR1-restricted T cells in mice and humans.. Nature Communications, 10 (1), pp.2243-2243. https://doi.org/10.1038/s41467-019-10198-w.
Access StatusOpen Access
Open Access at PMChttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC6529461
Mucosal-associated invariant T (MAIT) cells express an invariant TRAV1/TRAJ33 TCR-α chain and are restricted to the MHC-I-like molecule, MR1. Whether MAIT cell development depends on this invariant TCR-α chain is unclear. Here we generate Traj33-deficient mice and show that they are highly depleted of MAIT cells; however, a residual population remains and can respond to exogenous antigen in vitro or pulmonary Legionella challenge in vivo. These residual cells include some that express Trav1+ TCRs with conservative Traj-gene substitutions, and others that express Trav1- TCRs with a broad range of Traj genes. We further report that human TRAV1-2- MR1-restricted T cells contain both MAIT-like and non-MAIT-like cells, as judged by their TCR repertoire, antigen reactivity and phenotypic features. These include a MAIT-like population that expresses a public, canonical TRAV36+ TRBV28+ TCR. Our findings highlight the TCR diversity and the resulting potential impact on antigen recognition by MR1-restricted T cells.
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