Human blood MAIT cell subsets defined using MR1 tetramers
AuthorGherardin, NA; Souter, MNT; Koay, H-F; Mangas, KM; Seemann, T; Stinear, TP; Eckle, SBG; Berzins, SP; d'Udekem, Y; Konstantinov, IE; ...
Source TitleIMMUNOLOGY AND CELL BIOLOGY
University of Melbourne Author/sNeeson, Paul; Souter, Michael; Seemann, Torsten; Eckle, Sidonia; Berzins, Stuart; D'Udekem D'Acoz, Yves; Ritchie, David; Pellicci, Daniel; Uldrich, Adam; Godfrey, Dale; ...
AffiliationMedicine and Radiology
Microbiology and Immunology
Sir Peter MacCallum Department of Oncology
Document TypeJournal Article
CitationsGherardin, N. A., Souter, M. N. T., Koay, H. -F., Mangas, K. M., Seemann, T., Stinear, T. P., Eckle, S. B. G., Berzins, S. P., d'Udekem, Y., Konstantinov, I. E., Fairlie, D. P., Ritchie, D. S., Neeson, P. J., Pellicci, D. G., Uldrich, A. P., McCluskey, J. & Godfrey, D. I. (2018). Human blood MAIT cell subsets defined using MR1 tetramers. IMMUNOLOGY AND CELL BIOLOGY, 96 (5), pp.507-525. https://doi.org/10.1111/imcb.12021.
Access StatusOpen Access
Open Access at PMChttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446826
ARC Grant codeARC/DE170100407
Mucosal-associated invariant T (MAIT) cells represent up to 10% of circulating human T cells. They are usually defined using combinations of non-lineage-specific (surrogate) markers such as anti-TRAV1-2, CD161, IL-18Rα and CD26. The development of MR1-Ag tetramers now permits the specific identification of MAIT cells based on T-cell receptor specificity. Here, we compare these approaches for identifying MAIT cells and show that surrogate markers are not always accurate in identifying these cells, particularly the CD4+ fraction. Moreover, while all MAIT cell subsets produced comparable levels of IFNγ, TNF and IL-17A, the CD4+ population produced more IL-2 than the other subsets. In a human ontogeny study, we show that the frequencies of most MR1 tetramer+ MAIT cells, with the exception of CD4+ MAIT cells, increased from birth to about 25 years of age and declined thereafter. We also demonstrate a positive association between the frequency of MAIT cells and other unconventional T cells including Natural Killer T (NKT) cells and Vδ2+ γδ T cells. Accordingly, this study demonstrates that MAIT cells are phenotypically and functionally diverse, that surrogate markers may not reliably identify all of these cells, and that their numbers are regulated in an age-dependent manner and correlate with NKT and Vδ2+ γδ T cells.
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