Cobalt-Directed Assembly of Antibodies onto Metal-Phenolic Networks for Enhanced Particle Targeting.
AuthorZhang, W; Besford, QA; Christofferson, AJ; Charchar, P; Richardson, JJ; Elbourne, A; Kempe, K; Hagemeyer, CE; Field, MR; McConville, CF; ...
Source TitleNano Lett
University of Melbourne Author/sCaruso, Francesco
AffiliationChemical and Biomolecular Engineering
Document TypeJournal Article
CitationsZhang, W; Besford, QA; Christofferson, AJ; Charchar, P; Richardson, JJ; Elbourne, A; Kempe, K; Hagemeyer, CE; Field, MR; McConville, CF; Yarovsky, I; Caruso, F, Cobalt-Directed Assembly of Antibodies onto Metal-Phenolic Networks for Enhanced Particle Targeting., Nano Lett, 2020
Access StatusThis item is embargoed and will be available on 2021-03-10
The orientation-specific immobilization of antibodies onto nanoparticles, to preserve antibody-antigen recognition, is a key challenge in developing targeted nanomedicines. Herein, we report the targeting ability of metal-phenolic network (MPN)-coated gold nanoparticles with surface-physisorbed antibodies against respective antigens. The MPN coatings were self-assembled from metal ions (FeIII, CoII, CuII, NiII, or ZnII) cross-linked with tannic acid. Upon physisorption of antibodies, all particle systems exhibited enhanced association with target antigens, with CoII systems demonstrating more than 2-fold greater association. These systems contained more metal atoms distributed in a way to specifically interact with antibodies, which were investigated by molecular dynamics simulations. A model antibody fragment crystallizable (Fc) region in solution with CoII-tannic acid complexes revealed that the solvent-exposed CoII can directly coordinate to the histidine-rich portion of the Fc region. This one-pot interaction suggests anchoring of the antibody Fc region to the MPN on nanoparticles, allowing for enhanced targeting.
- Click on "Export Reference in RIS Format" and choose "open with... Endnote".
- Click on "Export Reference in RIS Format". Login to Refworks, go to References => Import References