dc.contributor.author | Battlay, Paul | |
dc.date.accessioned | 2020-04-20T14:15:14Z | |
dc.date.available | 2020-04-20T14:15:14Z | |
dc.date.issued | 2019 | |
dc.identifier.uri | http://hdl.handle.net/11343/237497 | |
dc.description | © 2019 Paul Battlay | |
dc.description.abstract | While understanding insecticide resistance in Drosophila melanogaster is informative for controlling pest insects that threaten agricultural yields and vector deadly diseases, it also serves as a powerful model of microevolution which can be interrogated with an exceptionally powerful genetic toolkit. The Drosophila Genetic Reference Panel (DGRP) provides the opportunity to study population-genetic signatures of natural selection in individuals that can be repeatedly measured for a range of phenotypes. In this work, genomic and transcriptomic data from the DGRP are compared with phenotypes from nine insecticidal compounds against the background of genome-wide signals of selection. The two most prominent signatures of selection in the population are attributable to insecticides from a single, widely-used chemical class, the organophosphates. Evidence suggests that insecticide-based selection is limited to these two loci, however the genetic bases of insecticide phenotypes appear to be complex. Insecticide-associated variation includes both structural effects through amino acid substitution and chimeric gene formation, and regulatory effects on transcript abundance by cis- and trans-acting factors. Resistance mechanisms exhibiting pleiotropic effects on insecticides from different chemical classes is found to be rare; one such case is correlated with constitutive, modular regulation of oxidative stress-related transcripts, the genetic basis of which is mapped to multiple trans-acting factors. Comparisons of the results from the DGRP with diverse population genomics data suggests that the outcomes of these analyses are applicable to populations of D. melanogaster worldwide. | |
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dc.subject | Drosophila melanogaster | |
dc.subject | insecticide resistance | |
dc.subject | quantitative genetics | |
dc.subject | DGRP | |
dc.subject | GWAS | |
dc.subject | TWAS | |
dc.subject | organophosphate | |
dc.subject | pyrethroid | |
dc.subject | azinphos-methyl | |
dc.subject | chlorantraniliprole | |
dc.subject | DDT | |
dc.subject | imidacloprid | |
dc.subject | malathion | |
dc.subject | permethrin | |
dc.subject | acetylcholinesterase | |
dc.subject | Cyp6a17 | |
dc.subject | Cyp6a23 | |
dc.subject | Cyp6g1 | |
dc.subject | Cyp6w1 | |
dc.subject | selective sweep | |
dc.subject | H12 | |
dc.subject | structural variation | |
dc.subject | copy-number variation | |
dc.title | The quantitative genetics of insecticide resistance in Drosophila melanogaster | |
dc.type | PhD thesis | |
melbourne.affiliation.department | School of BioSciences | |
melbourne.affiliation.faculty | Science | |
melbourne.thesis.supervisorname | Geoffrey Robin | |
melbourne.contributor.author | Battlay, Paul | |
melbourne.tes.fieldofresearch1 | 060411 Population, Ecological and Evolutionary Genetics | |
melbourne.tes.fieldofresearch2 | 060412 Quantitative Genetics (incl. Disease and Trait Mapping Genetics) | |
melbourne.tes.fieldofresearch3 | 060409 Molecular Evolution | |
melbourne.tes.fieldofresearch4 | 060408 Genomics | |
melbourne.accessrights | Open Access | |