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dc.contributor.authorBriffa, JF
dc.contributor.authorGrinfeld, E
dc.contributor.authorMathai, ML
dc.contributor.authorPoronnik, P
dc.contributor.authorMcAinch, AJ
dc.contributor.authorHryciw, DH
dc.date.accessioned2020-05-22T00:44:33Z
dc.date.available2020-05-22T00:44:33Z
dc.date.issued2015-02-05
dc.identifierpii: S0303-7207(14)00389-X
dc.identifier.citationBriffa, J. F., Grinfeld, E., Mathai, M. L., Poronnik, P., McAinch, A. J. & Hryciw, D. H. (2015). Acute leptin exposure reduces megalin expression and upregulates TGF beta 1 in cultured renal proximal tubule cells. Molecular and Cellular Endocrinology, 401 (C), pp.25-34. https://doi.org/10.1016/j.mce.2014.11.024.
dc.identifier.issn0303-7207
dc.identifier.urihttp://hdl.handle.net/11343/239178
dc.description.abstractIncreased leptin concentrations observed in obesity can lead to proteinuria, suggesting that leptin may play a role in obesity-related kidney disease. Obesity reduces activation of AMP-activated protein kinase (AMPK) and increases transforming growth factor-β1 (TGF-β1) expression in the kidney, leading to albuminuria. Thus we investigated if elevated leptin altered AMPK and TGF-β1 signaling in proximal tubule cells (PTCs). In opossum kidney (OK) PTCs Western blot analysis demonstrated that leptin upregulates TGF-β1 secretion (0.50 µg/ml) and phosphorylated AMPKα (at 0.25, and 0.50 µg/ml), and downregulates megalin expression at all concentrations (0.05–0.50 µg/ml). Using the AMPK inhibitor, Compound C, leptin exposure regulated TGF-β1 expression and secretion in PTCs via an AMPK mediated pathway. In addition, elevated leptin exposure (0.50 µg/ml) reduced albumin handling in OK cells independently of megalin expression. This study demonstrates that leptin upregulates TGF-β1, reduces megalin, and reduces albumin handling in PTCs by an AMPK mediated pathway.
dc.languageEnglish
dc.publisherElsevier
dc.titleAcute leptin exposure reduces megalin expression and upregulates TGF beta 1 in cultured renal proximal tubule cells
dc.typeJournal Article
dc.identifier.doi10.1016/j.mce.2014.11.024
melbourne.affiliation.departmentPhysiology
melbourne.source.titleMolecular and Cellular Endocrinology
melbourne.source.volume401
melbourne.source.issueC
melbourne.source.pages25-34
melbourne.elementsid934381
melbourne.openaccess.statusPublished version
melbourne.contributor.authorGriffith, Jessica
melbourne.contributor.authorMcAinch, Andrew
dc.identifier.eissn1872-8057
melbourne.conference.locationIreland
melbourne.accessrightsThis item is currently not available from this repository


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