Show simple item record

dc.contributor.authorBriffa, JF
dc.contributor.authorGrinfeld, E
dc.contributor.authorMathai, ML
dc.contributor.authorPoronnik, P
dc.contributor.authorMcAinch, AJ
dc.contributor.authorHryciw, DH
dc.identifierpii: S0303-7207(14)00389-X
dc.identifier.citationBriffa, J. F., Grinfeld, E., Mathai, M. L., Poronnik, P., McAinch, A. J. & Hryciw, D. H. (2015). Acute leptin exposure reduces megalin expression and upregulates TGF beta 1 in cultured renal proximal tubule cells. Molecular and Cellular Endocrinology, 401 (C), pp.25-34.
dc.description.abstractIncreased leptin concentrations observed in obesity can lead to proteinuria, suggesting that leptin may play a role in obesity-related kidney disease. Obesity reduces activation of AMP-activated protein kinase (AMPK) and increases transforming growth factor-β1 (TGF-β1) expression in the kidney, leading to albuminuria. Thus we investigated if elevated leptin altered AMPK and TGF-β1 signaling in proximal tubule cells (PTCs). In opossum kidney (OK) PTCs Western blot analysis demonstrated that leptin upregulates TGF-β1 secretion (0.50 µg/ml) and phosphorylated AMPKα (at 0.25, and 0.50 µg/ml), and downregulates megalin expression at all concentrations (0.05–0.50 µg/ml). Using the AMPK inhibitor, Compound C, leptin exposure regulated TGF-β1 expression and secretion in PTCs via an AMPK mediated pathway. In addition, elevated leptin exposure (0.50 µg/ml) reduced albumin handling in OK cells independently of megalin expression. This study demonstrates that leptin upregulates TGF-β1, reduces megalin, and reduces albumin handling in PTCs by an AMPK mediated pathway.
dc.titleAcute leptin exposure reduces megalin expression and upregulates TGF beta 1 in cultured renal proximal tubule cells
dc.typeJournal Article
melbourne.source.titleMolecular and Cellular Endocrinology
melbourne.openaccess.statusPublished version
melbourne.contributor.authorGriffith, Jessica
melbourne.contributor.authorMcAinch, Andrew
melbourne.accessrightsThis item is currently not available from this repository

Files in this item


There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record