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dc.contributor.authorLampugnani, ER
dc.contributor.authorMoller, IE
dc.contributor.authorCassin, A
dc.contributor.authorJones, DF
dc.contributor.authorKoh, PL
dc.contributor.authorRatnayake, S
dc.contributor.authorBeahan, CT
dc.contributor.authorWilson, SM
dc.contributor.authorBacic, A
dc.contributor.authorNewbigin, E
dc.identifierpii: PONE-D-13-27027
dc.identifier.citationLampugnani, E. R., Moller, I. E., Cassin, A., Jones, D. F., Koh, P. L., Ratnayake, S., Beahan, C. T., Wilson, S. M., Bacic, A. & Newbigin, E. (2013). In Vitro Grown Pollen Tubes of Nicotiana alata Actively Synthesise a Fucosylated Xyloglucan. PLOS ONE, 8 (10),
dc.description.abstractNicotiana alata pollen tubes are a widely used model for studies of polarized tip growth and cell wall synthesis in plants. To better understand these processes, RNA-Seq and de novo assembly methods were used to produce a transcriptome of N. alata pollen grains. Notable in the reconstructed transcriptome were sequences encoding proteins that are involved in the synthesis and remodelling of xyloglucan, a cell wall polysaccharide previously not thought to be deposited in Nicotiana pollen tube walls. Expression of several xyloglucan-related genes in actively growing pollen tubes was confirmed and xyloglucan epitopes were detected in the wall with carbohydrate-specific antibodies: the major xyloglucan oligosaccharides found in N. alata pollen grains and tubes were fucosylated, an unusual structure for the Solanaceae, the family to which Nicotiana belongs. Finally, carbohydrate linkages consistent with xyloglucan were identified chemically in the walls of N. alata pollen grains and pollen tubes grown in culture. The presence of a fucosylated xyloglucan in Nicotiana pollen tube walls was thus confirmed. The consequences of this discovery to models of pollen tube growth dynamics and more generally to polarised tip-growing cells in plants are discussed.
dc.titleIn Vitro Grown Pollen Tubes of Nicotiana alata Actively Synthesise a Fucosylated Xyloglucan
dc.typeJournal Article
melbourne.affiliation.departmentSchool of BioSciences
melbourne.affiliation.departmentMelbourne Veterinary School
melbourne.affiliation.departmentVeterinary Biosciences
melbourne.source.titlePLOS ONE
dc.rights.licenseCC BY
melbourne.contributor.authorCassin, Andrew
melbourne.contributor.authorWILSON, SARAH
melbourne.contributor.authorLampugnani, Edwin
melbourne.contributor.authorBacic, Anthony
melbourne.contributor.authorNewbigin, Edward
melbourne.contributor.authorMOLLER, ISABEL
melbourne.contributor.authorKoh, Poh
melbourne.contributor.authorRatnayake, Sunil
melbourne.contributor.authorBeahan, Cherie
melbourne.accessrightsAccess this item via the Open Access location

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