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    Lentiviral transduction of rat Sertoli cells as a means to modify gene expression.

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    Author
    Nicholls, PK; Stanton, PG; Rainczuk, KE; Qian, H; Gregorevic, P; Harrison, CA
    Date
    2012-10-01
    Source Title
    Spermatogenesis
    Publisher
    Informa UK Limited
    University of Melbourne Author/s
    Gregorevic, Paul; Qian, Hongwei
    Affiliation
    Physiology
    Metadata
    Show full item record
    Document Type
    Journal Article
    Citations
    Nicholls, P. K., Stanton, P. G., Rainczuk, K. E., Qian, H., Gregorevic, P. & Harrison, C. A. (2012). Lentiviral transduction of rat Sertoli cells as a means to modify gene expression.. Spermatogenesis, 2 (4), pp.279-284. https://doi.org/10.4161/spmg.22516.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/240786
    DOI
    10.4161/spmg.22516
    Open Access at PMC
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3521750
    Abstract
    Primary cell culture is an established and widely used technique to study Sertoli cell function in vitro. However, the relative difficulty of stably overexpressing or knocking down genes in Sertoli cell culture has limited progress in the field. In this technical report, we present a method to transduce 20 dpp rat Sertoli cell cultures with VSV-G pseudotyped lentiviral based vectors at a high rate (~80%), with stable reporter gene expression. Although high transgene expression is desirable, it was noted that at transduction rates > 60% inter-Sertoli cell tight junction integrity and, hence, Sertoli cell function, were transiently compromised. We envisage that this optimized procedure has the potential to stimulate Sertoli cell research, and motivate the use of Sertoli cells in various cell therapy applications.

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