Lentiviral transduction of rat Sertoli cells as a means to modify gene expression.

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Nicholls, PK; Stanton, PG; Rainczuk, KE; Qian, H; Gregorevic, P; Harrison, CADate
2012-10-01Source Title
SpermatogenesisPublisher
Informa UK LimitedAffiliation
PhysiologyMetadata
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Journal ArticleCitations
Nicholls, P. K., Stanton, P. G., Rainczuk, K. E., Qian, H., Gregorevic, P. & Harrison, C. A. (2012). Lentiviral transduction of rat Sertoli cells as a means to modify gene expression.. Spermatogenesis, 2 (4), pp.279-284. https://doi.org/10.4161/spmg.22516.Access Status
Open AccessOpen Access at PMC
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3521750Abstract
Primary cell culture is an established and widely used technique to study Sertoli cell function in vitro. However, the relative difficulty of stably overexpressing or knocking down genes in Sertoli cell culture has limited progress in the field. In this technical report, we present a method to transduce 20 dpp rat Sertoli cell cultures with VSV-G pseudotyped lentiviral based vectors at a high rate (~80%), with stable reporter gene expression. Although high transgene expression is desirable, it was noted that at transduction rates > 60% inter-Sertoli cell tight junction integrity and, hence, Sertoli cell function, were transiently compromised. We envisage that this optimized procedure has the potential to stimulate Sertoli cell research, and motivate the use of Sertoli cells in various cell therapy applications.
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