Show simple item record

dc.contributor.authorCaldow, MK
dc.contributor.authorHam, DJ
dc.contributor.authorTrieu, J
dc.contributor.authorChung, JD
dc.contributor.authorLynch, GS
dc.contributor.authorKoopman, R
dc.date.accessioned2020-07-03T03:01:33Z
dc.date.available2020-07-03T03:01:33Z
dc.date.issued2019-11-13
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000499449000001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=d4d813f4571fa7d6246bdc0dfeca3a1c
dc.identifierARTN 172
dc.identifier.citationCaldow, M. K., Ham, D. J., Trieu, J., Chung, J. D., Lynch, G. S. & Koopman, R. (2019). Glycine Protects Muscle Cells From Wasting in vitro via mTORC1 Signaling. FRONTIERS IN NUTRITION, 6, https://doi.org/10.3389/fnut.2019.00172.
dc.identifier.issn2296-861X
dc.identifier.urihttp://hdl.handle.net/11343/241309
dc.description.abstractGlycine supplementation can protect skeletal muscles of mice from cancer-induced wasting, but the mechanisms underlying this protection are not well-understood. The aim of this study was to determine whether exogenous glycine directly protects skeletal muscle cells from wasting. C2C12 muscle cells were exposed to non-inflammatory catabolic stimuli via two models: serum withdrawal (SF) for 48 h; or incubation in HEPES buffered saline (HBS) for up to 5 h. Cells were supplemented with glycine or equimolar concentrations of L-alanine. SF- and HBS-treated myotubes (with or without L-alanine) were ~20% and ~30% smaller than control myotubes. Glycine-treated myotubes were up to 20% larger (P < 0.01) compared to cells treated with L-alanine in both models of muscle cell atrophy. The mTORC1 inhibitor rapamycin prevented the glycine-stimulated protection of myotube diameter, and glycine-stimulated S6 phosphorylation, suggesting that mTORC1 signaling may be necessary for glycine's protective effects in vitro. Increasing glycine availability may be beneficial for muscle wasting conditions associated with inadequate nutrient intake.
dc.languageEnglish
dc.publisherFRONTIERS MEDIA SA
dc.titleGlycine Protects Muscle Cells From Wasting in vitro via mTORC1 Signaling
dc.typeJournal Article
dc.identifier.doi10.3389/fnut.2019.00172
melbourne.affiliation.departmentPhysiology
melbourne.source.titleFRONTIERS IN NUTRITION
melbourne.source.volume6
dc.rights.licenseCC BY
melbourne.elementsid1424843
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC6871541
melbourne.contributor.authorLynch, Gordon
melbourne.contributor.authorCaldow, Marissa
melbourne.contributor.authorKoopman, Rene
dc.identifier.eissn2296-861X
pubs.acceptance.date2019-10-25
melbourne.accessrightsOpen Access


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record