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dc.contributor.authorXu, J
dc.contributor.authorFalconer, C
dc.contributor.authorNguyen, Q
dc.contributor.authorCrawford, J
dc.contributor.authorMcKinnon, BD
dc.contributor.authorMortlock, S
dc.contributor.authorSenabouth, A
dc.contributor.authorAndersen, S
dc.contributor.authorChiu, HS
dc.contributor.authorJiang, L
dc.contributor.authorPalpant, NJ
dc.contributor.authorYang, J
dc.contributor.authorMueller, MD
dc.contributor.authorHewitt, AW
dc.contributor.authorPebay, A
dc.contributor.authorMontgomery, GW
dc.contributor.authorPowell, JE
dc.contributor.authorCoin, LJM
dc.date.accessioned2020-10-12T23:54:25Z
dc.date.available2020-10-12T23:54:25Z
dc.date.issued2019-12-19
dc.identifierpii: 10.1186/s13059-019-1852-7
dc.identifier.citationXu, J., Falconer, C., Nguyen, Q., Crawford, J., McKinnon, B. D., Mortlock, S., Senabouth, A., Andersen, S., Chiu, H. S., Jiang, L., Palpant, N. J., Yang, J., Mueller, M. D., Hewitt, A. W., Pebay, A., Montgomery, G. W., Powell, J. E. & Coin, L. J. M. (2019). Genotype-free demultiplexing of pooled single-cell RNA-seq. GENOME BIOLOGY, 20 (1), https://doi.org/10.1186/s13059-019-1852-7.
dc.identifier.issn1474-760X
dc.identifier.urihttp://hdl.handle.net/11343/247063
dc.description.abstractA variety of methods have been developed to demultiplex pooled samples in a single cell RNA sequencing (scRNA-seq) experiment which either require hashtag barcodes or sample genotypes prior to pooling. We introduce scSplit which utilizes genetic differences inferred from scRNA-seq data alone to demultiplex pooled samples. scSplit also enables mapping clusters to original samples. Using simulated, merged, and pooled multi-individual datasets, we show that scSplit prediction is highly concordant with demuxlet predictions and is highly consistent with the known truth in cell-hashing dataset. scSplit is ideally suited to samples without external genotype information and is available at: https://github.com/jon-xu/scSplit.
dc.languageEnglish
dc.publisherBMC
dc.titleGenotype-free demultiplexing of pooled single-cell RNA-seq
dc.typeJournal Article
dc.identifier.doi10.1186/s13059-019-1852-7
melbourne.affiliation.departmentSurgery (RMH)
melbourne.source.titleGenome Biology
melbourne.source.volume20
melbourne.source.issue1
dc.rights.licenseCC BY
melbourne.elementsid1427829
melbourne.contributor.authorPebay, Alice
dc.identifier.eissn1474-760X
pubs.acceptance.date2019-10-07
melbourne.accessrightsOpen Access


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