Functional screening of selective mitochondrial inhibitors of Plasmodium
AuthorGomez-Lorenzo, MG; Rodriguez-Alejandre, A; Moliner-Cubel, S; Martinez-Hoyos, M; Bahamontes-Rosa, N; Gonzalez del Rio, R; Rodenas, C; de la Fuente, J; Luis Lavandera, J; Garcia-Bustos, JF; ...
Source TitleInternational Journal for Parasitology: Drugs and Drug Resistance
PublisherELSEVIER SCI LTD
University of Melbourne Author/sGarcia-Bustos, Jose
Document TypeJournal Article
CitationsGomez-Lorenzo, M. G., Rodriguez-Alejandre, A., Moliner-Cubel, S., Martinez-Hoyos, M., Bahamontes-Rosa, N., Gonzalez del Rio, R., Rodenas, C., de la Fuente, J., Luis Lavandera, J., Garcia-Bustos, J. F. & Mendoza-Losana, A. (2018). Functional screening of selective mitochondrial inhibitors of Plasmodium. INTERNATIONAL JOURNAL FOR PARASITOLOGY-DRUGS AND DRUG RESISTANCE, 8 (2), pp.295-303. https://doi.org/10.1016/j.ijpddr.2018.04.007.
Access StatusOpen Access
Phenotypic screening has produced most of the new chemical entities currently in clinical development for malaria, plus many lead compounds active against Plasmodium falciparum asexual stages. However, lack of knowledge about the mode of action of these compounds delays and may even hamper their future development. Identifying the mode of action of the inhibitors greatly helps to prioritise compounds for further development as novel antimalarials. Here we describe a whole-cell method to detect inhibitors of the mitochondrial electron transport chain, using oxygen consumption as high throughput readout in 384-well plate format. The usefulness of the method has been confirmed with the Tres Cantos Antimalarial Compound Set (TCAMS). The assay identified 124 respiratory inhibitors in TCAMS, seven of which were novel anti-plasmodial chemical structures never before described as mitochondrial inhibitors.
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