Minerva Elements Records

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    Ultrasound-Stimulated Microbubbles Enhance Radiation-Induced Cell Killing
    Mccorkell, G ; Nakayama, M ; Feltis, B ; Piva, T ; Geso, M (Elsevier, 2022-12)
    Recent in vivo studies using ultrasound-stimulated microbubbles as a localized radiosensitizer have had impressive results. While in vitro studies have also obtained similar results using human umbilical vein endothelial cells (HUVEC), studies using other cell lines have had varying results. This study was aimed at investigating any increases in radiation-induced cell killing in vitro using two carcinoma lines not previously investigated before (metastatic follicular thyroid carcinoma cells [FTC-238] and non-small cell lung carcinoma cells [NCI-H727]), in addition to HUVEC. Cells were treated using a combination of 1.6% (v/v) microbubbles, ∼90 s of 2-MHz ultrasound (mechanical index = 0.8) and 0–6 Gy of kilovolt or MV X-rays. Cell viability assays obtained 72 h post-treatment were normalized to untreated controls, and analysis of variance was used to determine statistical significance. All cells treated with combined ultrasound-stimulated microbubbles and radiation exhibited decreased normalized survival, with statistically significant effects observed for the NCI-H727 cells. No statistically significant differences in effects were observed using kV compared with MV radiation. Further studies using increased microbubble concentrations may be required to achieve statistically significant results for the FTC-238 and HUVEC lines.
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    Beyond 'speech delay': Expanding the phenotype of BRPF1-related disorder
    Morison, LD ; Van Reyk, O ; Baker, E ; Ruaud, L ; Couque, N ; Verloes, A ; Amor, DJ ; Morgan, AT (Elsevier, 2024-04)
    Pathogenic variants in BRPF1 cause intellectual disability, ptosis and facial dysmorphism. Speech and language deficits have been identified as a manifestation of BRPF1-related disorder but have not been systematically characterized. We provide a comprehensive delineation of speech and language abilities in BRPF1-related disorder and expand the phenotype. Speech and language, and health and medical history were assessed in 15 participants (male = 10, median age = 7 years 4 months) with 14 BRPF1 variants. Language disorders were common (11/12), and most had mild to moderate deficits across receptive, expressive, written, and social-pragmatic domains. Speech disorders were frequent (7/9), including phonological delay (6/9) and disorder (3/9), and childhood apraxia of speech (3/9). All those tested for cognitive abilities had a FSIQ ≥70 (4/4). Participants had vision impairment (13/15), fine (8/15) and gross motor delay (10/15) which often resolved in later childhood, infant feeding impairment (8/15), and infant hypotonia (9/15). We have implicated BRPF1-related disorder as causative for speech and language disorder, including childhood apraxia of speech. Adaptive behavior and cognition were strengths when compared to other monogenic neurodevelopmental chromatin-related disorders. The universal involvement of speech and language impairment is noteable, relative to the high degree of phenotypic variability in BRPF1-related disorder.
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    Speech and language in DDX3X-neurodevelopmental disorder: A call for early augmentative and alternative communication intervention
    Forbes, EJ ; Morison, LD ; Lelik, F ; Howell, T ; Debono, S ; Goel, H ; Burger, P ; Mandel, J-L ; Geneviève, D ; Amor, DJ ; Morgan, AT (Wiley, 2024-02-29)
    Pathogenic variants in DDX3X are associated with neurodevelopmental disorders. Communication impairments are commonly reported, yet specific speech and language diagnoses have not been delineated, preventing prognostic counseling and targeted therapies. Here, we characterized speech and language in 38 female individuals, aged 1.69-24.34 years, with pathogenic and likely pathogenic DDX3X variants (missense, n = 13; nonsense, n = 12; frameshift, n = 7; splice site, n = 3; synonymous, n = 2; deletion, n = 1). Standardized speech, language, motor, social, and adaptive behavior assessments were administered. All participants had gross motor deficits in infancy (34/34), and fine motor deficits were common throughout childhood (94%; 32/34). Intellectual disability was reported in 86% (24/28) of participants over 4 years of age. Expressive, receptive, and social communication skills were, on average, severely impaired. However, receptive language was significantly stronger than expressive language ability. Over half of the assessed participants were minimally verbal (66%; 22/33; range = 2 years 2 months-24 years 4 months; mean = 8 years; SD = 6 years) and augmented speech with sign language, gestures, or digital devices. A quarter of the cohort had childhood apraxia of speech (25%; 9/36). Despite speech and language impairments, social motivation was a relevant strength. Many participants used augmentative and alternative communication (AAC), underscoring the need for early, tailored, and comprehensive AAC intervention.
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    The Delivery of Person-Centered Care for People Living With Dementia in Residential Aged Care: A Systematic Review and Meta-Analysis
    Berkovic, D ; Macrae, A ; Gulline, H ; Horsman, P ; Soh, S-E ; Skouteris, H ; Ayton, D ; Heyn, PC (OXFORD UNIV PRESS INC, 2023-05-05)
    BACKGROUND AND OBJECTIVES: Person-centred care is the gold standard of care for people living with dementia, yet few systematic reviews have detailed how it is delivered in practice. This mixed-methods review aimed to examine the delivery of person-centred care, and its effectiveness, for people living with dementia in residential aged care. RESEARCH DESIGN AND METHODS: A systematic review and meta-analysis. Eligible studies were identified across four databases. Quantitative and qualitative studies containing data on person-centred care delivered to people with dementia living in residential aged care were included. Meta-analysis using a random effects model was conducted where more than three studies measured the same outcome. A narrative meta-synthesis approach was undertaken to categorise verbatim participant quotes into representative themes. Risk of bias was undertaken using quality appraisal tools from the Joanna Briggs Institute. RESULTS: Forty-one studies were identified for inclusion. There were 34 person-centred care initiatives delivered, targeting 14 person-centred care outcomes. Three outcomes could be pooled. Meta-analyses demonstrated no reduction in agitation (standardised mean difference -0.27, 95% CI -0.58, 0.03), improvement in quality of life (standardised mean difference -0.63, 95% CI -1.95, 0.70), or reduced neuropsychiatric symptoms (mean difference -1.06, 95% CI -2.16, 0.05). Narrative meta-synthesis revealed barriers (for example, time constraints) and enablers (for example, staff collaboration) to providing person-centred care from a staff perspective. DISCUSSION AND IMPLICATIONS: The effectiveness of person-centred care initiatives delivered to people with dementia in residential aged care is conflicting. Further high-quality research over an extended time is required to identify how person-centred care can be best implemented to improve resident outcomes.
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    Identification of cellulose nitrate based adhesive repairs in archaeological pottery of the University of Melbourne's Middle Eastern archaeological pottery collection using portable FTIR-ATR spectroscopy and PCA
    Noake, E ; Lau, D ; Nel, P (BMC, 2017-01-19)
    A previous study of the Cypriot pottery collection housed in the Ian Potter Museum of Art at the University of Melbourne found approximately two-thirds of adhesive repairs are primarily composed of cellulose nitrate (CN). This is of concern as CN has a limited lifespan (6–20 years), which has implications for the strategic management of the collection. To gain a greater understanding of the prevalence of CN based adhesive repairs in the archaeological context, the original FTIR-ATR spectroscopic survey was extended to incorporate the University’s Middle Eastern archaeological pottery collection. Micro-samples were removed from artefacts using acetone swabs. Analysis of adhesive FTIR spectra identified CN to be present not only as the primary polymer in approximately one-tenth of repairs, but also as a secondary polymer in poly(vinyl acetate) (PVAc) and acrylic adhesive formulations, observed as a weak peak at ~1656 cm−1. CN’s secondary presence in PVAc adhesive formulations is demonstrated using principal component analysis (PCA) and the diphenylamine spot test for CN. Re-analysis of adhesive IR spectra from the Cypriot pottery collection found CN to be present as a secondary polymer in this collection as well. It is concluded a combined methodology of collecting and identifying FTIR-ATR spectra of adhesive repairs from archaeological pottery collections followed by PCA analysis bring to light adhesive formulations which can inform the management of pottery collections.
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    Tea: An Alternative Adsorbent for the Preservation of Cellulose Triacetate Film
    Nel, P ; Bell, J ; Newnham, M (Australian Institute for the Conversation of Cultural Material Inc, 2017)
    Cellulose triacetate (CTA) film, the main film base of the Twentieth Century, is inherently unstable, affected by autocatalytic deterioration through hydrolysis. The release of, and subsequent exposure to, acetic acid known as ‘vinegar syndrome’ accelerates deterioration, placing all cellulose acetate materials at risk or actively deteriorating. Preservation techniques rely on cold storage to slow deterioration or microenvironments with adsorbent materials to remove corrosives and/or pollutants. However, commercially available adsorbents can be expensive and difficult to access. This research investigated the potential for tea and tea waste to act as an alternative, low cost, accessible adsorbent for the preservation of CTA film. Adsorption capabilities of various tea varieties and treatments were compared with activated charcoal, silica gel and molecular sieves. Testing established tea as an effective adsorbent of water and acetic acid vapour, with an aversion to adsorption of the plasticiser dibutyl-phthalate. Use of tea waste also involves additional cost, sustainability and accessibility benefits along with lessened corrosive potential. These findings support tea as a potentially viable alternative adsorbent for the preservation of CTA film, requiring further research into optimum application systems.
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    A preliminary investigation into the influence of archaeological material on the yellowing of polyethylene storage bags
    Thompson, K ; Nel, P (Routledge, 2021)
    Concerns around the degradation of plastics have been part of conservation discourse for decades. The spotlight is usually on art and objects, and conservation and display materials, however it could be argued that a significant volume of the plastics in museums is associated with storage bags. This study asked whether the condition of plastic storage bags might be influenced by what is stored inside them. If specific materials can be identified as more likely to affect plastic degradation, museums may have a lead-indicator for efficiently monitoring storage risks. This case study developed a methodology for applying multivariate analysis to collected data to answer this question. A subset of polyethylene self-seal bags used to pack archaeological material from the ‘Casselden Place’ assemblage at Museums Victoria was evaluated. Objective data were combined with subjective assessment of bag degradation features gathered during a collection survey and interrogated using multivariate statistical analysis. Results indicate (1) different levels of yellowing are associated with particular plastic bag stocks and (2) ceramic, slate and tile finds are more likely than other materials to be contained within yellower bags. The research points to future enquiry and demonstrates this methodology shows promise for extension to other large cultural datasets.
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    Global, but not chondrocyte-specific, MT1-MMP deficiency in adult mice causes inflammatory arthritis
    Xia, X-D ; Gill, G ; Lin, H ; Roth, DM ; Wang, X-J ; Su, F-Y ; Alabi, A ; Alexiou, M ; Zhang, Z ; Wang, G-Q ; Graf, D ; Zhang, D-W (ELSEVIER, 2023-09)
    Membrane-type I metalloproteinase (MT1-MMP/MMP14) plays a key role in various pathophysiological processes, indicating an unaddressed need for a targeted therapeutic approach. However, mice genetically deficient in Mmp14 show severe defects in development and growth. To investigate the possibility of MT1-MMP inhibition as a safe treatment in adults, we generated global Mmp14 tamoxifen-induced conditional knockout (Mmp14kd) mice and found that MT1-MMP deficiency in adult mice resulted in severe inflammatory arthritis. Mmp14kd mice started to show noticeably swollen joints two weeks after tamoxifen administration, which progressed rapidly. Mmp14kd mice reached a humane endpoint 6 to 8 weeks after tamoxifen administration due to severe arthritis. Plasma TNF-α levels were also significantly increased in Mmp14kd mice. Detailed analysis revealed chondrocyte hypertrophy, synovial fibrosis, and subchondral bone remodeling in the joints of Mmp14kd mice. However, global conditional knockout of MT1-MMP in adult mice did not affect body weight, blood glucose, or plasma cholesterol and triglyceride levels. Furthermore, we observed substantial expression of MT1-MMP in the articular cartilage of patients with osteoarthritis. We then developed chondrocyte-specific Mmp14 tamoxifen-induced conditional knockout (Mmp14chkd) mice. Chondrocyte MT1-MMP deficiency in adult mice also caused apparent chondrocyte hypertrophy. However, Mmp14chkd mice did not exhibit synovial hyperplasia or noticeable arthritis, suggesting that chondrocyte MT1-MMP is not solely responsible for the onset of severe arthritis observed in Mmp14kd mice. Our findings also suggest that highly cell-type specific inhibition of MT1-MMP is required for its potential therapeutic use.
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    Surveys of Plastics in Post-1950 Non-published Book Collections
    Chu, C ; Barrett, M ; Bunn, S ; Zilio, F ; Bell, J ; Nel, P (De Gruyter, 2023-03-03)
    Research over the past three decades has demonstrated that certain plastics in cultural materials are inherently unstable, displaying short lifespans and accelerating the degradation of neighbouring collection materials. Knowledge of the conservation of plastics is increasingly common in museum settings. However, less information is available on conserving plastics found in paper-based collections, and even less guidance on the materials and deterioration of plastic components found in book and document bindings. As plastics have been present in popular bookbinding materials since the mid-twentieth century, collection care professions require knowledge and methods for preserving these materials entering book collections. The aim of this paper is to determine strategies for the care of post-1950s books containing plastic. Collection surveys were conducted to determine the materials, structures, and degradation patterns of non-published books found in archive and archive-like settings at the South Australian Museum, the Art Gallery of New South Wales, and the Grimwade Centre for Cultural Materials Conservation at the University of Melbourne. A methodology combining condition reporting and infrared spectroscopy identified six plastic polymers in 35 binding styles that are summarised as 10 binding types. Recommendations are made for the use of preventive storage strategies responding to four categories of damage.
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    Identification of polymer-based artefacts from the former Wheeler Residence at the Mernda archaeological site in Victoria, Australia: A comparison of attenuated total reflectance and reflectance spectroscopic techniques
    Wong, SSH ; Stuart, B ; Kim, C ; Nel, P (International Council of Museums, 2021)
    The purpose of this investigation was to identify the polymers in artefacts recovered from archaeological excavations at the former Wheeler Residence using attenuated total reflectance Fourier transform infrared and reflectance spectroscopy. The results from both techniques are compared and discussed. Identification will assist with the long-term management and storage of these polymer-based artefacts. Evaluation of these two sampling techniques included whether the physical characteristics and polymer type of the artefacts favoured the use of one technique over the other. The inherent challenges of archaeological artefacts such as cataloguing conventions, awkwardly shaped fragments and soil encrustations complicated the analysis. Of the 270 samples analysed, 67% were identified as containing ten different types of polymers, with the remaining 33% consisting of unidentified polymers, encrusted polymers in which only soil bands or other materials such as glass were identified. Although reflectance achieved better results for certain types of artefacts, it also revealed limitations. The identified polymers are compatible with the proposed occupation of the site from 1852 to the 1970s.